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Purification of GST-tagged Protein
GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.
Performing a Purification and On-column Refolding of an Insoluble Histidine-tagged Protein
This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap™ FF columns and ÄKTAprime from Cytiva.
Purification of Membrane Proteins
This page shows how to perform a purification of His-tagged membrane proteins.
Practical Considerations for IEX Separation
This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.
Affinity Chromatography Troubleshooting
This page shows how to solve practical problems that may occur when running an affinity chromatography column.