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Purification of Membrane Proteins
This page shows how to perform a purification of His-tagged membrane proteins.
Selection of Purification Equipment
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
Non-volatile and Volatile Buffer Systems
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
Ion Exchange Chromatography Troubleshooting
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
Practical Considerations for IEX Separation
This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.