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Purification or Removal of Serine Proteases, such as Thrombin and Trypsin, and Zymogens
This page shows how to Purify or remove serine proteases with a Benzamidine
Sepharose 4 Fast Flow, Benzamidine Sepharose 4 Fast Flow from Cytiva.
Troubleshooting of Cleavage Methods
This page shows troubleshooting strategies for cleavage methods using Cytiva products.
Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification
Cleavage and Purification of GST-Tagged Protein Eluted from GSTrap
This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.
Cleavage and Purification of GST-Tagged Protein Bound to GSTrap
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
Purifcation or Removal of Serine Proteases, e.g. Thrombin and Trypsin, and Zymogens
This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from Cytiva.
Characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (High Sub) Media and Columns
This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.
Principles and Standard Conditions for Different Purification Techniques
This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.
Converting from Flow Velocity to Volumetric Flow Rates
This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.
Affinity Chromatography Troubleshooting
This page shows how to solve practical problems that may occur when running an affinity chromatography column.