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Showing 1-29 of 29 results for "H7891" within Papers
HCC ligation: rapid and specific DNA construction with blunt ended DNA fragments.
J A Murray
Nucleic acids research, 14(24), 10118-10118 (1986-12-22)
B Grünewald
The Journal of comparative neurology, 404(1), 114-126 (1999-01-14)
The anatomy of gamma-aminobutyric acid (GABA)-immunoreactive, recurrent feedback neurons in the mushroom body (MB) of the honeybee, Apis mellifera, was investigated by using intraneuropilar injections of cobalt ions and light microscopic techniques. Each MB contains approximately 110 GABA-immunoreactive neurons, and
Protection of megabase DNA from shearing.
R T Kovacic et al.
Nucleic acids research, 23(19), 3999-4000 (1995-10-11)
Y Tsubamoto et al.
The Journal of biological chemistry, 276(5), 2979-2985 (2000-11-09)
Hexamminecobalt(III) (HAC) chloride was found to have a potent inhibitory effect on glucose-induced insulin secretion from pancreatic islets. HAC at 2 mm inhibited the secretion in response to 22.2 mm glucose by 90% in mouse islets. Perifusion experiments revealed that
Wei Yu et al.
Nature communications, 11(1), 5239-5239 (2020-10-18)
The alternative non-homologous end-joining (NHEJ) pathway promotes DNA double-strand break (DSB) repair in cells deficient for NHEJ or homologous recombination, suggesting that it operates at all stages of the cell cycle. Here, we use an approach in which DNA breaks
Isabelle Bougie et al.
Biochimica et biophysica acta, 1794(1), 50-60 (2008-10-22)
West Nile virus (WNV) is a member of the Flaviviridae family which includes a number of important human pathogens. The WNV NS5 protein harbors an RNA-dependent RNA polymerase activity which is required both for replication and transcription of the viral
J D Rush et al.
Biochemistry, 27(16), 5876-5884 (1988-08-09)
Two sites for electron transfer have been proposed for plastocyanin: one near the copper ion and the other close to the acid patch formed by residues 42-45. Calculations of electrostatic properties of spinach plastocyanin and ionic strength dependences of electron-transfer
Dynamic nature of B to Z transition: role of DNA supercoiling and Z-DNA binding protein.
N Ramesh et al.
Indian journal of biochemistry & biophysics, 25(6), 542-547 (1988-12-01)
Meredith Newby Lambert et al.
RNA (New York, N.Y.), 11(11), 1688-1700 (2005-10-26)
Helix (H)27 from Escherichia coli 16S ribosomal (r)RNA is centrally located within the small (30S) ribosomal subunit, immediately adjacent to the decoding center. Bacterial 30S subunit crystal structures depicting Mg(2+) binding sites resolve two magnesium ions within the vicinity of
M C Whitby et al.
The Journal of biological chemistry, 273(31), 19729-19739 (1998-07-25)
The RecG protein of Escherichia coli is a junction-specific DNA helicase that drives branch migration of Holliday intermediates in genetic recombination and DNA repair. The reaction was investigated using synthetic X-junctions. RecG dissociates X-junctions to flayed duplex products, although DNA
Amin Ahmed Ouameur et al.
The Journal of biological chemistry, 279(40), 42041-42054 (2004-07-31)
Biogenic polyamines, such as putrescine, spermidine, and spermine are small organic polycations involved in numerous diverse biological processes. These compounds play an important role in nucleic acid function due to their binding to DNA and RNA. It has been shown
K M Sullivan et al.
Journal of molecular biology, 193(2), 397-404 (1987-01-20)
We have made a comparative study of the kinetics of cruciform extrusion by a salt-dependent (S-type) cruciform in the presence of a variety of metal ions and related species. We find that the nature of the cation present has a
Pinpinat Malaikaew et al.
Journal of photochemistry and photobiology. B, Biology, 103(3), 251-255 (2011-05-03)
In this study, a new small-molecule-based reagent was designed to recognize and bind to specific site in protein. A new pyrenyl probe, d-biotinyl-1(1-pyrene)methylamide (Py-biotin) was designed and synthesized by coupling of d-biotin to 1(1-pyrene)methylamine hydrochloride. Binding studies and site-specific photocleavage
G Soslau et al.
Nucleic acids research, 14(18), 7237-7252 (1986-09-25)
These studies employed the synthetic linear DNA, poly dGdC, in the B and cobalt hexammine chloride (Co)-induced Z form to determine the effect of conformation on protein-DNA interactions. The rate of the reaction of the restriction endonucleases, Hha I and
K E Rao et al.
Journal of biomolecular structure & dynamics, 7(2), 335-345 (1989-10-01)
Interaction of the DNA binding nonintercalators Netropsin, Distamycin and the mPD derivative with Z-DNA has been studied. It has been found that environmental factors like the solvent and added cations significantly modulate the interaction of these ligands with Z-DNA. However
Brian A Todd et al.
Biophysical journal, 94(12), 4775-4782 (2008-03-11)
By combining single-molecule magnetic tweezers and osmotic stress on DNA assemblies, we separate attractive and repulsive components of the total intermolecular interaction between multivalent cation condensed DNA. Based on measurements of several different cations, we identify two invariant properties of
Tumpa Sarkar et al.
Nucleic acids research, 33(1), 143-151 (2005-01-11)
The condensation of nucleic acids into well-defined particles is an integral part of several approaches to artificial cellular delivery. Improvements in the efficiency of nucleic acid delivery in vivo are important for the development of DNA- and RNA-based therapeutics. Presently
Z Gdaniec et al.
Biochemistry, 37(6), 1505-1512 (1998-03-04)
The ferritin IRE, a highly conserved (96-99% in vertebrates) mRNA translation regulatory element in animal mRNA, was studied by molecular modeling (using MC-SYM and DOCKING) and by NMR spectroscopy. Cobalt(III) hexammine was used to model hydrated Mg2+. IRE isoforms in
Michael G Poirier et al.
Journal of cellular biochemistry, 85(2), 422-434 (2002-04-12)
We show that the chromatin in mitotic chromosomes can be drastically overcompacted or unfolded by temporary shifts in ion concentrations. By locally 'microspraying' reactants from micron-size pipettes, while simultaneously monitoring the size of and tension in single chromosomes, we are
D Hanahan
Journal of molecular biology, 166(4), 557-580 (1983-06-05)
Factors that affect the probability of genetic transformation of Escherichia coli by plasmids have been evaluated. A set of conditions is described under which about one in every 400 plasmid molecules produces a transformed cell. These conditions include cell growth
Christine C Conwell et al.
Biochemistry, 43(18), 5380-5387 (2004-05-05)
Millimolar concentrations of divalent cations are shown to affect the size of toroids formed when DNA is condensed by multivalent cations. The origins of this effect were explored by varying the order in which MgCl(2) was added to a series
Tong Zhang et al.
PloS one, 7(8), e43872-e43872 (2012-09-07)
Crystal structures of Thermotoga maritima magnesium transporter CorA, reported in 2006, revealed its homo-pentameric constructions. However, the structure of the highly conserved extracellular interhelical loops remains unsolved, due to its high flexibility. We have explored the configurations of the loops
Yee Siew Choong et al.
Journal of molecular graphics & modelling, 29(6), 834-842 (2011-03-05)
The high typhoid incidence rate in developing and under-developed countries emphasizes the need for a rapid, affordable and accessible diagnostic test for effective therapy and disease management. TYPHIDOT®, a rapid dot enzyme immunoassay test for typhoid, was developed from the
J S Kieft et al.
Structure (London, England : 1993), 5(5), 713-721 (1997-05-15)
Solvated metal ions are critical for the proper folding and function of RNA. Despite the importance of these ions, the details of specific metal ion-RNA interactions are poorly understood. The crystal structure of a group I intron ribozyme domain characterized
D C Tessier et al.
Analytical biochemistry, 158(1), 171-178 (1986-10-01)
Despite its unique ability to ligate single-stranded DNA molecules, T4 RNA ligase has so far seen little use in molecular biology due to long reaction times, modest yields, and apparent inability to promote ligation of long oligodeoxyribonucleotides. We describe here
K L Kindle
Proceedings of the National Academy of Sciences of the United States of America, 87(3), 1228-1232 (1990-02-01)
By using a method in which cell-wall-deficient Chlamydomonas reinhardtii cells were agitated in the presence of DNA, glass beads, and polyethylene glycol, nuclear transformation rates of approximately 10(3) transformants per micrograms of plasmid DNA were achieved. The nitrate reductase gene
T Allers et al.
Nucleic acids research, 28(2), e6-e6 (1999-12-22)
The Holliday junction is a central intermediate in genetic recombination. This four-stranded DNA structure is capable of spontaneous branch migration, and is lost during standard DNA extraction protocols. In order to isolate and characterize recombination intermediates that contain Holliday junctions
P M Littlewood et al.
Brain research, 445(1), 165-170 (1988-03-29)
Intracellular injection of hexamminecobaltic chloride into a neurone causes characteristic changes to the structure of its mitochondria. This provides a simple technique to label neuronal profiles for examination of their ultrastructure. Synaptic structures are not affected by hexamminecobaltic ions.
Filip Senigl et al.
Cell reports, 29(12), 3902-3915 (2019-12-19)
Somatic hypermutation (SHM) introduces point mutations into immunoglobulin (Ig) genes but also causes mutations in other parts of the genome. We have used lentiviral SHM reporter vectors to identify regions of the genome that are susceptible ("hot") and resistant ("cold")
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