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Showing 1-30 of 45 results for "H9773" within Papers
Michael D Scahill et al.
Molecular and biochemical parasitology, 157(1), 73-82 (2007-11-17)
The limited repertoire of drug-resistance markers imposes a serious obstacle to genetic manipulation of Trypanosoma brucei. Here we describe experiments with a fusion protein that allows positive selection for genome integration followed by CRE recombinase-mediated excision of the marker cassette
Jingli Yang et al.
Molecular biology reports, 40(1), 281-288 (2012-10-16)
An efficient transformation protocol was developed for Agrobacterium-mediated transformation of Phellodendron amurense Rupr. for using explants from mature seeds. The binary vector pCAMBIA1303, which contained hygromycin phosphotransferase (hptII) as a selectable marker gene and β-glucuronidase (GUS) as a reporter gene
Masatoshi Aida et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(19), 7784-7789 (2013-04-24)
Somatic hypermutation (SHM) requires not only the expression of activation-induced cytidine deaminase, but also transcription in the target regions. However, how transcription guides activation-induced cytidine deaminase in targeting SHM to the Ig genes is not fully understood. Here, we found
A I Emets et al.
TSitologiia i genetika, 47(3), 14-20 (2013-07-05)
The results on in vitro culture establishment, plantlet regeneration and rooting of Camelina sativa cultivar sample Peremozhets and cultivar Mirazh are presented. Effective concentrations of sterilizing agents and duration of plant material treatment were estimated. Phytohormone ratio, sucrose concentration in
Sung Ki Cho et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(40), 15629-15634 (2008-09-24)
Abscission is a developmental program that results in the active shedding of infected or nonfunctional organs from a plant body. Here, we establish a signaling pathway that controls abscission in Arabidopsis thaliana from ligand, to receptors, to downstream effectors. Loss
Matthew S Scarnati et al.
Alcohol (Fayetteville, N.Y.), 87, 97-109 (2020-06-21)
The acute and chronic effects of alcohol on the brain and behavior are linked to alterations in inhibitory synaptic transmission. Alcohol's most consistent effect at the synaptic level is probably a facilitation of γ-aminobutyric acid (GABA) release, as seen from
Ben-Jiang Ma et al.
PLoS pathogens, 7(9), e1002200-e1002200 (2011-09-13)
The HIV-1 gp41 envelope (Env) membrane proximal external region (MPER) is an important vaccine target that in rare subjects can elicit neutralizing antibodies. One mechanism proposed for rarity of MPER neutralizing antibody generation is lack of reverted unmutated ancestor (putative
M Cruz Muñoz-Centeno et al.
FEMS yeast research, 12(5), 511-520 (2012-03-16)
Using a yeast two-hybrid screen, we isolated a gene from Schizosaccharomyces pombe, whose product interacts with Mpg1, a GDP-mannose-1-phosphate guanylyltransferase involved in the maintenance of cell wall integrity and glycosylation. We have designated this gene mpg2 based on its similarity
Goetz Hensel et al.
BMC plant biology, 12, 171-171 (2012-09-26)
While the genetic transformation of the major cereal crops has become relatively routine, to date only a few reports were published on transgenic triticale, and robust data on T-DNA integration and segregation have not been available in this species. Here
Masayuki Nakamura et al.
Current microbiology, 65(2), 176-182 (2012-05-16)
Agrobacterium tumefaciens-mediated transformation (AtMT) has become a common technique for DNA transformation of yeast and filamentous fungi. In this study, we first established a protocol of AtMT for the phytopathogenic fungus Colletotrichum sansevieriae. Binary T-DNA vector containing the hygromycin B
Yajun Xi et al.
Methods in molecular biology (Clifton, N.J.), 581, 53-59 (2009-09-22)
Switchgrass (Panicum virgatum L.) is a highly productive warm-season C4 species that is being developed into a dedicated biofuel crop. This chapter describes a protocol that allows the generation of transgenic switchgrass plants by Agrobacterium tumefaciens-mediated transformation. Embryogenic calluses induced
Yusheng Zhao et al.
Nature, 583(7818), 825-829 (2020-07-17)
Temperature is a key factor in the growth and development of all organisms1,2. Plants have to interpret temperature fluctuations, over hourly to monthly timescales, to align their growth and development with the seasons. Much is known about how plants respond
I M G Padilla et al.
Plant cell reports, 29(11), 1203-1213 (2010-07-21)
Plant transformation protocols generally involve the use of selectable marker genes for the screening of transgenic material. The bacterial gene nptII, coding for a neomycin phosphotransferase, and the hpt gene, coding for a hygromycin phosphotransferase, are frequently used. These enzymes
Detection of probable marker-free transgene-positive rice plants resistant to rice tungro disease from backcross progenies of transgenic Pusa Basmati 1.
Somnath Roy et al.
Journal of genetics, 91(2), 213-218 (2012-09-04)
Angela Saez et al.
The Plant cell, 20(11), 2972-2988 (2008-11-27)
Abscisic acid (ABA) has an important role for plant growth, development, and stress adaptation. HYPERSENSITIVE TO ABA1 (HAB1) is a protein phosphatase type 2C that plays a key role as a negative regulator of ABA signaling; however, the molecular details
Milena Szafraniec et al.
Dalton transactions (Cambridge, England : 2003), 41(32), 9728-9736 (2012-07-14)
Catalytic RNA molecules (ribozymes) have often been used for the testing of interactions of antibiotics with ribonucleic acids. We showed that the impact of capreomycin and hygromycin B on delta ribozyme catalysis might change dramatically, from stimulation to inhibition, depending
Hongbo Liu et al.
Fungal genetics and biology : FG & B, 45(5), 646-656 (2007-12-07)
The general transcriptional repressor Tup1p is known to influence cell development in many fungi. To determine whether the Tup1p ortholog (WdTup1p) of Wangiella dermatitidis also influences cellular development in this melanized, polymorphic human pathogen, the gene (WdTUP1) that encodes this
Félix de Carpentier et al.
Frontiers in plant science, 11, 242-242 (2020-03-27)
Chlamydomonas reinhardtii is a model unicellular organism for basic or biotechnological research, such as the production of high-value molecules or biofuels thanks to its photosynthetic ability. To enable rapid construction and optimization of multiple designs and strains, our team and
Sandra Gouiaa et al.
Plant molecular biology, 79(1-2), 137-155 (2012-03-15)
Abiotic stress tolerance of plants is a very complex trait and involves multiple physiological and biochemical processes. Thus, the improvement of plant stress tolerance should involve pyramiding of multiple genes. In the present study, we report the construction and application
Xiang Liu et al.
Plant cell reports, 32(1), 77-87 (2012-11-01)
KEY MESSAGE : Efficient Agrobacterium -mediated genetic transformation for investigation of genetic and molecular mechanisms involved in inflorescence architectures in Cornus species. Cornus canadensis is a subshrub species in Cornus, Cornaceae. It has recently become a favored non-model plant species
M Dutt et al.
Plant cell reports, 29(11), 1251-1260 (2010-08-17)
A method for the genetic transformation of several citrus cultivars is described, including cultivars observed to be recalcitrant to conventional epicotyl-mediated transformation. Embryogenic cell suspension cultures, established from unfertilized ovules were used as target tissues for Agrobacterium-mediated transformation. Several modifications
Tsuyoshi Nakagawa et al.
Journal of bioscience and bioengineering, 104(1), 34-41 (2007-08-19)
We developed a new series of binary vectors useful for Gateway cloning to facilitate transgenic experiments in plant biotechnology. The new system, Gateway Binary Vectors (pGWBs) realized efficient cloning, constitutive expression using the cauliflower mosaic virus (CaMV) 35S promoter and
Yuko Konishi et al.
BioTechniques, 53(3), 141-152 (2012-09-12)
Gene targeting in a broad range of human somatic cell lines has been hampered by inefficient homologous recombination. To improve this technology and facilitate its widespread application, it is critical to first have a robust and efficient research system for
Mauricio Niklitschek et al.
Methods in molecular biology (Clifton, N.J.), 898, 219-234 (2012-06-20)
Generally two selection markers are required to obtain homozygous mutations in a diploid background, one for each gene copy that is interrupted. In this chapter is described a method that allows the double gene deletions of the two copies of
Daisuke Iino et al.
Journal of structural biology, 183(1), 76-85 (2013-06-12)
Aminoglycoside 4-phosphotransferase-Ia (APH(4)-Ia)/Hygromycin B phosphotransferase (Hph) inactivates the aminoglycoside antibiotic hygromycin B (hygB) via phosphorylation. The crystal structure of the binary complex of APH(4)-Ia with hygB was recently reported. To characterize substrate recognition by the enzyme, we determined the crystal
Akane Kubota et al.
Bioscience, biotechnology, and biochemistry, 77(1), 167-172 (2013-01-08)
The thallus, the gametophyte body of the liverwort Marchantia polymorpha, develops clonal progenies called gemmae that are useful in the isolation and propagation of isogenic plants. Developmental timing is critical to Agrobacterium-mediated transformation, and high transformation efficiency has been achieved
Sabine Gruber et al.
BMC research notes, 5, 641-641 (2012-11-20)
Species of the fungal genus Trichoderma are important industrial producers of cellulases and hemicellulases, but also widely used as biocontrol agents (BCAs) in agriculture. In the latter function Trichoderma species stimulate plant growth, induce plant defense and directly antagonize plant
Hyojin Kim et al.
PloS one, 8(2), e56476-e56476 (2013-02-27)
The ability to enrich cells with targeted mutations greatly facilitates the process of using engineered nucleases, including zinc-finger nucleases and transcription activator-like effector nucleases, to construct such cells. We previously used surrogate reporters to enrich cells containing nuclease-induced mutations via
Iksung Jin et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(23), 9137-9142 (2012-05-24)
Whereas short-term (minutes) facilitation at Aplysia sensory-motor neuron synapses is presynaptic, long-term (days) facilitation involves synaptic growth, which requires both presynaptic and postsynaptic mechanisms. How are the postsynaptic mechanisms recruited, and when does that process begin? We have been investigating
Inja Radman et al.
PloS one, 8(10), e76019-e76019 (2013-10-17)
We report a simple, cost-effective, scalable and efficient method for creating transgenic Caenorhabditis elegans that requires minimal hands-on time. The method combines biolistic bombardment with selection for transgenics that bear a hygromycin B resistance gene on agar plates supplemented with
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