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Showing 1-30 of 34 results for "L9774" within Papers
Margarita Sobol et al.
Histochemistry and cell biology, 134(6), 631-641 (2010-11-11)
In this study we present an optimized method of high-pressure freezing and automated freeze-substitution of cultured human cells, followed by LR White embedding, for subsequent immunolabeling. Also, the influence of various conditions of the freeze-substitution procedures such as temperature, duration
R Y Osamura et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 48(7), 885-891 (2000-06-17)
Plastic embedding has been used to localize various antigens in conjunction with immunohistochemistry. Peptide hormones have been among the antigens that have been studied extensively. Recent application of water-soluble plastics such as LR White and Lowicryl has extended the ranges
Quantitative changes in metallothionein expression in target cell-types in the gills of turbot (Scophthalmus maximus) exposed to Cd, Cu, Zn and after a depuration treatment
Alvarado N E, et al.
Aquatic Toxicology (Amsterdam, Netherlands), 77(1), 64-77 (2006)
T K Bhattacharyya et al.
Biotechnic & histochemistry : official publication of the Biological Stain Commission, 75(1), 7-14 (2000-05-16)
Histology of plastic embedded undecalcified bone represents a challenging problem to the histotechnologist. We outline here an exploration of LR White resin as a suitable medium for histologic study of undecalcified rat tibia. A procedure was developed for light microscopy
Ubiquitin E3 ligase Wwp1 negatively regulates osteoblast function by inhibiting osteoblast differentiation and migration
Shu L, et al.
Journal of Bone and Mineral Research, 28(9), 1925-1935 (2013)
A Matsuno et al.
The Histochemical journal, 30(2), 105-109 (1999-04-07)
An improved new method for the simultaneous visualization of mRNA and encoded protein in LR White resin-embedded specimens is described. This pre-embedding electron microscopical in situ hybridization (procedure) localized rat growth hormone mRNA specifically as high electron-density products on the
G R Owen et al.
Cell biology international, 25(12), 1251-1259 (2001-12-26)
Visualisation of cell adhesion patterns by scanning electron microscopy requires special preparation and labelling. The membranes and cytoplasm must be removed, without damaging the antigen, to facilitate antibody access to vinculin in the focal adhesions. Low beam energy imaging is
H S Brilakis et al.
Current eye research, 22(3), 235-244 (2001-07-20)
To determine whether the preservation of the extracellular matrix and the ultrastructural appearance of the trabecular meshwork are affected by different histologic processing protocols and embedding media. Conventionally used epoxy resins such as Araldite require complete dehydration of tissue, while
Yuko Sakai et al.
Archives of histology and cytology, 68(5), 337-347 (2006-03-01)
Although hydrophilic acrylic resins including LR White have been widely utilized as embedding media for immunocytochemical use, the constituents of tissues are often extracted by the resin monomer during the infiltration process of the embedment, resulting in a discernible impairment
A A Al-Hazzaa et al.
The Histochemical journal, 30(12), 897-902 (1999-04-01)
A series of techniques based on LR White resin are described, which permit the use of an anti-histone antibody for the in situ localization of DNA fragmentation characteristic of apoptosis at both the light and the electron microscope level. The
Salem S Ghrebi et al.
Microscopy research and technique, 70(7), 555-562 (2007-03-24)
The staining of intracellular antigenic sites in postembedded samples is a challenging problem. Deterioration of antigenicity and limited antibody accessibility to the antigen are commonly encountered on account of processing steps. In this study preservation of the antigen was achieved
Vendula Strádalová et al.
Histochemistry and cell biology, 130(5), 1047-1052 (2008-09-18)
A protocol for high-pressure freezing and LR White embedding of mammalian cells suitable for fine ultrastructural studies in combination with immunogold labelling is presented. HeLa S3 cells enclosed in low-temperature gelling agarose were high-pressure frozen, freeze-substituted in acetone, and embedded
I His et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 49(12), 1525-1536 (2001-11-29)
Flax fibers have been the subject of many biochemical studies, which revealed that cellulose and pectins are the major constituents of their walls. In contrast, little is known about the location of these polymers within the walls of mature fibers
R Yang et al.
European cells & materials, 6, 57-71 (2004-01-15)
Trabecular bone is routinely analysed by histomorphological-histometrical and immunohistochemical techniques as means of assessing the differentiation status of bone deposition and growth. Currently few embedding resins exist for which both morphological and immunohistochemical analyses can be performed on mineralised tissue.
Olivier Gros et al.
Acta histochemica, 110(5), 427-431 (2008-01-12)
Hydrophilic resins present the advantage of making possible both hybridization experiments involving either antibodies or oligonucleotide probes and ultrastructural observations. Whereas various embedding protocols are available, only very few concern flat-embedded preparations. In this study we describe an easy protocol
V V Philimonenko et al.
European journal of histochemistry : EJH, 46(4), 359-364 (2003-02-25)
The purpose of this study was to compare two electron microscopy embedding media - LR White and Unicryl - with regard to cell morphologyical and immunohistochemical preservation properties for the study of fixation-sensitive nuclear antigens. Human cervical carcinoma (HeLa) cells
Mechanisms of desensitization to parathyroid hormone in human osteoblast-like SaOS-2 cells
Fukayama S, et al.
Endocrinology, 131(4), 1757-1769 (1992)
LR White Acrylic Resin
Yeung EC and Huang BQ
Plant Microtechniques and Protocols, 103-103 (2015)
Adam J Granger et al.
eLife, 9 (2020-07-03)
The mouse cerebral cortex contains neurons that express choline acetyltransferase (ChAT) and are a potential local source of acetylcholine. However, the neurotransmitters released by cortical ChAT+ neurons and their synaptic connectivity are unknown. We show that the nearly all cortical
Distribution of arabinogalactan proteins during microsporogenesis in the anther of Bellis perennis L.(Asteraceae).
Chudzik B
ACTA BIOLOGICA CRACOVIENSIA Series Botanica, 56, 49-49 (2014)
C J von Ruhland et al.
Journal of microscopy, 240(2), 130-134 (2010-10-16)
The utility of LR White sections as slot grid support films for the examination of thin resin-embedded tissue sections by transmission electron microscopy was investigated and compared with traditional formvar-carbon films. Throughout a variety of staining procedures, which involved the
Beta-glucuronidase and hexosaminidase are marker enzymes for different compartments of the endo-lysosomal system in mussel digestive cells.
Izagirre U
Cell and Tissue Research, 335, 441-441 (2009)
S H Brorson
Micron (Oxford, England : 1993), 29(6), 439-443 (1999-03-11)
The purpose of this study was to examine the intensity of the immunogold labeling of kappa light chains as single molecules and as parts of whole immunoglobulin molecules in LR-White sections and epoxy sections both practically and theoretically. Human renal
O Laboux et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 51(1), 61-67 (2002-12-28)
Methylmethacrylate (MMA) embedding of undecalcified bone is routinely employed for histomorphometric analyses. Although MMA-embedded bone has been used for immunolabeling at the light microscopic level after removal of the resin, there are no such reports for electron microscopy. The aim
Katherine Luby-Phelps et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 51(3), 271-274 (2003-02-18)
We have developed a procedure for visualizing GFP expression in fixed tissue after embedding in LR White. We find that GFP fluorescence survives fixation in 4% paraformaldehyde/0.1% glutaraldehyde and can be visualized directly by fluorescence microscopy in unstained, 1 microm
R G Richards et al.
Cell biology international, 25(12), 1237-1249 (2001-12-26)
A new immunogold labelling method for the visualisation of vinculin, an integral protein in focal adhesions of cells, is reported. Quantification of vinculin is indicative of substrate cytocompatibility (cytocompatibility is one aspect of biocompatibility; it is the cellular response to
Cathy N John et al.
Frontiers in microbiology, 10, 1021-1021 (2019-06-25)
Background/Objectives: With mucocutaneous candidiasis being highly prevalent in HIV patients, the emergence of fluconazole-resistant Candida species forms a major challenge in treating and eradicating these infections. The objective of this study was to establish the antifungal activity of K21, a
C R Hann et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 49(4), 475-482 (2001-03-22)
Antigen retrieval (AR) methods can unmask tissue antigens that have been altered by fixation, processing, storage, or resin interactions. This is particularly important in the study of archival tissues, because primary fixatives and storage times may vary among specimens. We
Maria Palmieri et al.
Microscopy research and technique, 68(2), 80-84 (2005-10-18)
Acrylic LR White is used preferentially for many research applications because it possesses unique advantages over other commercially available resin types. LR White has low toxicity; its low viscosity and hydrophilic properties enable it to infiltrate specimens with comparative ease
Shinji Yano et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 51(2), 199-204 (2003-01-21)
Immunoelectron microscopy using chromogranin A-specific antibodies has been proposed as an efficient technique for identification of secretory granules (SGs) in tumor cells with evidence of apparent neuroendocrine differentiation. Using an antigen retrieval (AR) method, we succeeded in immunolabeling SGs with
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