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General Care and Use of Supelco HPLC Columns
clean, particle-free, and
use a guard column with an in-line frit filter.
Pressure — Continuous monitoring of system pressure will alert
you to changes that may require you to perform preventative
maintenance
The Samplicity®System Delivers High-Performance Multi-sample Filtration
increase productivity and
data quality generated by liquid chromatography.
Samples filtered using the Samplicity™ filtration
system will benefit from low extractables and efficient
particulate retention
Mass Spectrometry: Effects of Extractables from Syringe Filters
: Effects of
Extractables from Syringe Filters
Data Sheet
Liquid chromatography mass spectrom-
etry (LC-MS) and gas chromatography
mass spectrometry (GC-MS) are being
increasingly used in analysis
Low-extractable Millex Samplicity®filters: ideal for preparing samples for sensitive HPLC detectors
HPLC analysis. Given the high sensitivity of
high performance liquid chromatography (HPLC) and ultra-high pressure liquid chromatography (UHPLC) analyses, it
is critical to ensure that the filtered sample
The Samplicity®Filtration System Streamlines Chromatography Sample Preparation
Samplicity Chromatography SP
Data Sheet
Abstract
Recent innovations that have accelerated chromatographic separation, such as ultrahigh performance liquid
chromatography (UHPLC) and monolithic
Bulletin - PROT20LC
maximum pressure at 30 psi. Higher pressures may •
compress the resin and reduce column efficiency and lifetime. A low/medium
pressure FPLC is recommended with this column. A high pressure liquid
chromatography
Mass Spectrometry(MS)
can be coupled to various separation techniques such as liquid chromatography (LC), thin layer
chromatography (TLC), or gas chromatography (GC) where the hyphenation with LC is by far the
most
Eshmuno®CPS Chromatography Resin User Guide
results. An
error will result in inaccurate packed bed compression, giving
rise to high or low operating pressures and possibly poor
HETP/As values.
Compression Factors
Column Size
Recommended Compression
Mobius®Single-Use Technology Supporting ADC Processing
dispersion of the injected solution. Tight
asymmetries and low HETP values are easily achievable (Figure 10).
Figure 7:
SU chromatography
system pressure
performance at up to
8 L/min
Figure 11:
Minimum
Eshmuno®CMX Chromatography Resin
column to the
chromatography system.
10. Start pumping at a low flow rate (2 mL/
min) and prime the column inlet line.
11. Open the bottom outlet and make a
liquid to liquid connection to the
Eshmuno® CP-FT Chromatography Resin User Guide
the column to the
chromatography system.
10. Start pumping at a low flow rate (2 mL/
min) and prime the column inlet line.
11. Open the bottom outlet and make a
liquid to liquid connection to the
Product Information Sheet - H7788
The cartridge is suitable for Low
Pressure Liquid Chromatography Systems (LPLC),
Medium Pressure Liquid Chromatography Systems
(MPLC), Fast Pressure Liquid Chromatography
Systems (FPLC), and for use
Product Information Sheet - H7163
The cartridge is suitable for Low
Pressure Liquid Chromatography Systems (LPLC),
Medium Pressure Liquid Chromatography Systems
(MPLC), Fast Pressure Liquid Chromatography
Systems (FPLC), and for use
Product Information Sheet - SEP090
fitting on the HPLC tubing.
2. Adapting the column (M6 fitting) to non-metric
medium/low pressure liquid chromatography
systems
• Option 1: female 1/4-28 to male 10-32 one-
piece adapter (Catalog Number
Product Information Sheet - SEP120
fitting on the HPLC tubing.
2. Adapting the column (M6 fitting) to non-metric
medium/low pressure liquid chromatography
systems
• Option 1: female 1/4-28 to male 10-32 one-
piece adapter (Catalog Number
Product Information Sheet - SEP040
fitting on the HPLC tubing.
2. Adapting the column (M6 fitting) to non-metric
medium/low pressure liquid chromatography
systems
• Option 1: female 1/4-28 to male 10-32 one-
piece adapter (Catalog Number
Fuel the future - ANALYTICAL SOLUTIONS FOR THE PETROCHEMICAL INDUSTRY
Gas Chromatography (GC) 06
Columns 06
Sharp peaks for water 08
GC Accessories 09
Purity under pressure
Gas Purification 09
Tame the unstable
High Performance Liquid
Chromatography (HPLC
Product Information Sheet - SEP030
fitting on the HPLC tubing.
2. Adapting the column (M6 fitting) to non-metric
medium/low pressure liquid chromatography
systems
• Option 1: female 1/4-28 to male 10-32 one-
piece adapter (Catalog Number
Product Information Sheet - H8286
lock fittings and
accompanying adapters are suitable for Low Pressure
Liquid Chromatography Systems (LPLC), Medium
Pressure Liquid Chromatography Systems (MPLC), and
for use with syringes.
HIS-Select
Product Information Sheet - SEP080
fitting on the HPLC tubing.
2. Adapting the column (M6 fitting) to non-metric
medium/low pressure liquid chromatography
systems
• Option 1: female 1/4-28 to male 10-32 one-
piece adapter (Catalog Number
Chromatography Instrumental Techniques
temperature, pressure, different sizes in length, diameter)
We can differentiate between the techniques based on the mobile and the stationary phase.
- Gas Chromatography (GC): gas + column
- Liquid Chromatography
Brochure: HPLC Method Development Guide
reaching HPLC
system pressure limits. Alternatively, coupling of multiple
columns can be a means of achieving high efficiencies at
normal pressures. In addition, a low column back pressure
puts you into the
ChromaBolt Prepacked Chromatography Columns - Ion Exchange Resins - User Guide
processing
High back pressure Flow rate greater than the resin recommended flow rate
High viscosity liquid being flowed
through column Reduce flow rates
Low temperature liquid being flowed
through
PharmPrep®P Sorbent
equivalent to 15 g per kg stationary phase.
Preparative Liquid Chromatography
PharmPrep™ P sorbent for preparative liquid
chromatography (LC) is a spherical, porous silica
sorbent characterized by
Product Information Sheet - SEP020
fitting on the HPLC tubing.
2. Adapting the column (M6 fitting) to non-metric
medium/low pressure liquid chromatography
systems
• Option 1: female 1/4-28 to male 10-32 one-
piece adapter (Catalog Number
Ascentis Express OH5 Column Care and Use Sheet
discussed in
this document) or for aqueous normal phase chromatography with the typi-
cal mobile phases for hydrophilic interactive liquid chromatography (HILIC)
of polar basic, acidic, zwitterionic, or neutral
Eshmuno®A Affinity Chromatography Media Operating Instructions
column to the chromatography system.
10. Start flow with the pump at a low flow rate (2 mL/min) and prime
the column inlet line.
11. Open the bottom outlet and make a liquid to liquid connection
Superior Performance of HIS-Select HF Nickel Affinity Gel
is well suited for larger
scale processing or small-scale fast performance liquid
chromatography (FPLC) applications at pressures up to
200 psi. As with all HIS-Select resins, the HF matrix demon-
strates
Eshmuno® CPX User Guide
the column to the chromatography system.
10. Start pumping at a low flow rate (2 mL/min) and prime the
column inlet line.
11. Open the bottom outlet and make a liquid to liquid connection
to the
SAMS Instructions for Use
, or any other low-molecular water-soluble organic solvent. Such solutions will swell the membrane
excessively with drastic irreversible decrease in ion-exchange selectivity and pressure resistance.
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