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M5379
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Showing 1-30 of 40 results for "M5379" within Papers
Neurochemical research, 40(2), 274-283 (2014-07-30)
Hepatic encephalopathy (HE) represents a neuropsychiatric syndrome, which evolves as a consequence of a low grade cerebral edema and a concomitant oxidative/nitrosative stress response. Ephrin receptors (EphR) and their ligands (ephrins) regulate astrocytic glutamate uptake and gliotransmitter release thereby governing
International journal of molecular sciences, 22(18) (2021-09-29)
Glutamine and lipids are two important components of proliferating cancer cells. Studies have demonstrated that glutamine synthetase (GS) boosts glutamine-dependent anabolic processes for nucleotide and protein synthesis, but the role of GS in regulating lipogenesis remains unclear. This study identified
The Journal of neuroscience : the official journal of the Society for Neuroscience, 30(4), 1288-1300 (2010-01-29)
The neurotransmitter glutamate is recycled through an astrocytic-neuronal glutamate-glutamine cycle in which synaptic glutamate is taken up by astrocytes, metabolized to glutamine, and transferred to neurons for conversion back to glutamate and subsequent release. The extent to which neuronal glutamate
PLoS genetics, 7(8), e1002229-e1002229 (2011-08-20)
Although significant variations in the metabolic profiles exist among different cells, little is understood in terms of genetic regulations of such cell type-specific metabolic phenotypes and nutrient requirements. While many cancer cells depend on exogenous glutamine for survival to justify
The European journal of neuroscience, 6(2), 287-291 (1994-02-01)
The effects of apomorphine, a D1-, D2-dopamine receptor agonist, on the extracellular concentrations of excitatory amino acids, glutamic and aspartic acids, and on that of their precursor, glutamine, were investigated using an intracerebral perfusion system. Apomorphine produced a concentration-related rise
Neurochemical research, 24(1), 51-59 (1999-02-11)
Ammonia causes astrocyte swelling which is abrogated by methionine sulfoximine (MSO). Since myo-inositol is an important osmolyte, we investigated the effects of ammonia and MSO on myoinositol flux in cultured astrocytes for periods up to 72 hours. Uptake of myo-inositol
Glutamine deprivation initiates reversible assembly of mammalian rods and rings.
Cellular and Molecular Life Sciences, 71(15), 2963-2973 (2014)
Gender medicine, 9(6), 524-535 (2012-12-12)
To investigate the role of sex and the role of ammonia and amino acid metabolism, specifically the activity of glutamine synthetase, in survival and disease progression in amyotrophic lateral sclerosis. We tested treatment with methionine sulfoximine (MSO) on the lifespan
PloS one, 6(3), e18233-e18233 (2011-04-13)
The membrane-proximal external region (MPER) of the HIV-1 gp41 consists of epitopes for the broadly cross-neutralizing monoclonal antibodies 2F5 and 4E10. However, antigens containing the linear sequence of these epitopes are unable to elicit potent and broad neutralizing antibody responses
Pivotal role of glutamine synthetase in ammonia detoxification
Hepatology, 65, 281-293 (2017)
Biotechnology progress, 33(1), 17-25 (2016-11-01)
In Lonza Biologics' GS Gene Expression System™, recombinant protein-producing GS-CHOK1SV cell lines are generated by transfection with an expression vector encoding both GS and the protein product genes followed by selection in MSX and glutamine-free medium. MSX is required to
Improving the efficiency of CHO cell line generation using glutamine synthetase gene knockout cells.
Biotechnology and bioengineering, 109(4), 1007-1015 (2011-11-10)
Although Chinese hamster ovary (CHO) cells, with their unique characteristics, have become a major workhorse for the manufacture of therapeutic recombinant proteins, one of the major challenges in CHO cell line generation (CLG) is how to efficiently identify those rare
Nitrate transport and not photoinhibition limits growth of the freshwater Cyanobacterium synechococcus species PCC 6301 at low temperature.
Plant Physiology, 119, 785?794-785?794 (1999)
Effect of Methionine Sulfoximine on the Accumulation of Ammonia in C3 and C4 Leaves
Plant Physiology, 71, 177-181 (1983)
PLoS genetics, 18(3), e1010101-e1010101 (2022-03-04)
Glutamine analogs are potent suppressors of general glutamine metabolism with anti-cancer activity. 6-diazo-5-oxo-L-norleucine (DON) is an orally available glutamine analog which has been recently improved by structural modification for cancer treatment. Here, we explored the chemogenomic landscape of DON sensitivity
mAbs, 11(5), 965-976 (2019-05-03)
Chinese hamster ovary (CHO) cells are the biopharmaceutical industry's primary means of manufacturing therapeutic proteins, including monoclonal antibodies. The major challenge in cell line development for the production of recombinant biopharmaceuticals lies in generating and isolating rare high-producing stable clones
Biochemistry, 48(4), 779-785 (2009-01-28)
The initial events of HIV-1 cell infection involve the sequential binding of the viral envelope glycoprotein gp120 to the cellular CD4 receptor and the coreceptor, usually CCR5 or CXCR4. Binding to the coreceptor triggers the chain of events that culminates
PloS one, 10(7), e0133595-e0133595 (2015-07-25)
In the HIV vaccine field, there is a need to produce highly immunogenic forms of the Env protein with the capacity to trigger broad B and T-cell responses. Here, we report the generation and characterization of a chimeric HIV-1 gp120
Molecular brain, 14(1), 132-132 (2021-09-05)
The medium-chain fatty acids octanoic acid (C8) and decanoic acid (C10) are gaining attention as beneficial brain fuels in several neurological disorders. The protective effects of C8 and C10 have been proposed to be driven by hepatic production of ketone
Neurochemical research, 22(9), 1145-1150 (1997-09-01)
Excessive activation of glutamate receptors via the N-methyl-D-aspartate (NMDA) subtype appears to play a role in the sequence of cellular events which lead to irreversible ischemic damage to neurons. Furthermore, NMDA receptor activation induces a stimulation of ornithine decarboxylase (ODC)
iScience, 25(3), 103877-103877 (2022-03-05)
Malectins from the oligosaccharyltransferase (OST) complex in the endoplasmic reticulum (ER) of animal cells are involved in ER quality control and contribute to the Unfolded Protein Response (UPR). Malectins are not found in plant cells, but malectin-like domains (MLDs) are
L-methionine-DL-sulfoximine induces massive efflux of glutamine from cortical astrocytes in primary culture
European journal of psychotraumatology, 182(3), 587-589 (1990)
Biochimica et biophysica acta. Molecular cell research, 1867(2), 118571-118571 (2019-11-11)
The heterotrimeric transcription factor NF-Y binds to CCAAT boxes of genes of glutamine metabolism. We set out to study the role of the regulatory NF-YA subunit in this pathway. We produced U2OS and A549 clones stably overexpressing -OE- the two
The Biochemical journal, 462(2), 329-335 (2014-06-12)
Human BChE (butyrylcholinesterase) protects against the toxicity of organophosphorus nerve agents and pesticides. BChE purified from human plasma is limited and pathogen carry-over is a concern. Unlike the native BChE tetrameric complex with a residence time of days, rBChE (recombinant
Current pharmaceutical biotechnology, 11(3), 233-240 (2010-03-10)
Mammalian cell lines are important host cells for the industrial production of pharmaceutical proteins owing to their capacity for correct folding, assembly and post-translational modification. In particular, Chinese hamster ovary (CHO) cells are the most dependable host cells for the
The Journal of nutrition, 129(1), 57-62 (1999-01-23)
The importance of glutamine synthetase (GS) for cell proliferation was examined in rat intestinal crypt cells (IEC-6) by inhibiting its activity with 10 mmol/L methionine sulfoximine (MS) at varying extracellular glutamine (Q) concentrations. In uninhibited cultures, cell number, protein, and
European journal of pharmacology, 235(2-3), 189-196 (1993-04-28)
L-Methionine-D,L-sulfoximine (MSO) (25 micrograms) infused locally into the dorsal raphe nucleus (DRN) induced a progressive decrease in body temperature in conscious restrained rats kept at an ambient temperature of 23 degrees C. Pretreatment with (+/-)-pindolol (3 mg/kg s.c.) significantly attenuated
Developmental cell, 56(16), 2329-2347 (2021-08-25)
Mammalian preimplantation embryos follow a stereotypic pattern of development from zygotes to blastocysts. Here, we use labeled nutrient isotopologue analysis of small numbers of embryos to track downstream metabolites. Combined with transcriptomic analysis, we assess the capacity of the embryo
FEMS microbiology letters, 151(2), 163-168 (1997-06-15)
To examine further the role of the enzyme glutamine synthetase in Dictyostelium discoideum we report here the effects of a specific glutamine synthetase inhibitor, methionine sulfoximine, on the growth and differentiation of this organism. Vegetative AX3 cells grown in the
The EMBO journal, 40(6), e106583-e106583 (2021-01-19)
Plasmodium falciparum (Pf) is a major cause of human malaria and is transmitted by infected Anopheles mosquitoes. The initial asymptomatic infection is characterized by parasite invasion of hepatocytes, followed by massive replication generating schizonts with blood-infective merozoites. Hepatocytes can be
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