Search Within
M8250
Applied Filters:
Keyword:'M8250'
Showing 1-30 of 115 results for "M8250" within Papers
Guilhem Reyt et al.
Current biology : CB, 30(20), 4103-4111 (2020-08-29)
Casparian strips (CSs) are cell wall modifications of vascular plants restricting extracellular free diffusion into and out of the vascular system [1]. This barrier plays a critical role in controlling the acquisition of nutrients and water necessary for normal plant
Alicia Villacampa et al.
Plants (Basel, Switzerland), 10(4) (2021-05-01)
Clinorotation was the first method designed to simulate microgravity on ground and it remains the most common and accessible simulation procedure. However, different experimental settings, namely angular velocity, sample orientation, and distance to the rotation center produce different responses in
Taras P Pasternak et al.
Plant physiology, 129(4), 1807-1819 (2002-08-15)
Culturing leaf protoplast-derived cells of the embryogenic alfalfa (Medicago sativa subsp. varia A2) genotype in the presence of low (1 microM) or high (10 microM) 2, 4-dichlorophenoxyacetic acid (2,4-D) concentrations results in different cell types. Cells exposed to high 2,4-D
Benjamin P Holder et al.
PloS one, 6(3), e14767-e14767 (2011-04-02)
In 2007, the A/Brisbane/59/2007 (H1N1) seasonal influenza virus strain acquired the oseltamivir-resistance mutation H275Y in its neuraminidase (NA) gene. Although previous studies had demonstrated that this mutation impaired the replication capacity of the influenza virus in vitro and in vivo
Guangjie Li et al.
Bio-protocol, 7(13), e2387-e2387 (2017-07-05)
Root system architecture depends on nutrient availability. A symptom of iron (Fe) toxicity in plants is stunted root growth, yet little is known about the effects of excess Fe on lateral root (LR) development. To better understand how nutrient signals
Baoda Han et al.
Plant physiology, 182(2), 1052-1065 (2019-12-07)
Plasma membrane (PM) depolarization functions as an initial step in plant defense signaling pathways. However, only a few ion channels/transporters have been characterized in the context of plant immunity. Here, we show that the Arabidopsis (Arabidopsis thaliana) Na+:K+:2Cl- (NKCC) cotransporter
Shuai Jin et al.
Molecular cell, 79(5), 728-740 (2020-07-30)
Cytosine base editors (CBEs) generate C-to-T nucleotide substitutions in genomic target sites without inducing double-strand breaks. However, CBEs such as BE3 can cause genome-wide off-target changes via sgRNA-independent DNA deamination. By leveraging the orthogonal R-loops generated by SaCas9 nickase to
Morten Egevang Jørgensen et al.
Bio-protocol, 7(20), e2581-e2581 (2017-10-20)
Xenopus laevis oocytes are a widely used model system for characterization of heterologously expressed secondary active transporters. Historically, researchers have relied on detecting transport activity by measuring accumulation of radiolabeled substrates by scintillation counting or of fluorescently labelled substrates by
Takehiro Yokota et al.
Proteins, 66(2), 272-278 (2006-10-28)
Human JNK stimulatory phosphatase-1 (JSP-1) is a novel member of dual specificity phosphatases. A C-terminus truncated JSP-1 was expressed in Escherichia coli and was crystallized using the sitting-drop vapor diffusion method. Thin-plate crystals obtained at 278 K belong to a
Kenji Sugioka et al.
Developmental cell, 46(3), 257-270 (2018-07-24)
Cell division axes during development are specified in different orientations to establish multicellular assemblies, but the mechanisms that generate division axis diversity remain unclear. We show here that patterns of cell contact provide cues that diversify cell division orientation by
Susann K J Ludwig et al.
Analytical and bioanalytical chemistry, 406(27), 6857-6866 (2014-06-29)
Current contaminant and residue monitoring throughout the food chain is based on sampling, transport, administration, and analysis in specialized control laboratories. This is a highly inefficient and costly process since typically more than 99% of the samples are found to
Matthew J Townsend et al.
mAbs, 7(5), 812-819 (2015-07-16)
A micro-capillary film has been developed that offers the potential for an at-line analytical tool for rapid aggregate analysis during biopharmaceutical antibody production. A non-porous walled micro-capillary film (NMCF) with cation exchange functionality was demonstrated to act as a chromatography
Christian Engelbrekt et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 11(13), 2844-2853 (2010-08-18)
Monodisperse platinum nanoparticles (PtNPs) were synthesized by a green recipe. Glucose serves as a reducing agent and starch as a stabilization agent to protect the freshly formed PtNP cores in buffered aqueous solutions. Among the ten buffers studied, 2-(N-morpholino)ethanesulfonic acid
Jessica R Ray et al.
ACS applied materials & interfaces, 7(21), 11117-11126 (2015-05-06)
Polyamide (PA) semipermeable membranes typically used for reverse osmosis water treatment processes are prone to fouling, which reduces the amount and quality of water produced. By synergistically coupling the photothermal and bactericidal properties of graphene oxide (GO) nanosheets, gold nanostars
Xu Yu et al.
Biosensors & bioelectronics, 66, 520-526 (2014-12-17)
A novel highly sensitive colorimetric assay for DNA detection using cascade amplification strategy based on hybridization chain reaction and enzyme-induced metallization was established. The DNA modified superparamagnetic beads were demonstrated to capture and enrich the target DNA in the hybridization
Ekta G Ahuja et al.
Acta crystallographica. Section D, Biological crystallography, 60(Pt 6), 1129-1131 (2004-05-26)
Phenazines are broad-spectrum antibiotic metabolites produced by organisms such as Pseudomonas and Streptomyces. Phenazines have been shown to enhance microbial competitiveness and the pathogenic potential of the organisms that synthesize them. PhzA (163 residues, approximate molecular weight 18.7 kDa) is
Youssef Abdalla et al.
Journal of nanobiotechnology, 19(1), 60-60 (2021-02-28)
Approximately 80% of brain tumours are gliomas. Despite treatment, patient mortality remains high due to local metastasis and relapse. It has been shown that transferrin-functionalised porous silicon nanoparticles (Tf@pSiNPs) can inhibit the migration of U87 glioma cells. However, the underlying
Pasi S Vuorinen et al.
Journal of chromatography. A, 990(1-2), 45-52 (2003-04-11)
A contactless conductivity detector integrated into the capillary cassette of Agilent (3D)CE equipment is described. The detector is user-friendly, compact and easily modified. The UV detector of the (3D)CE equipment is available parallel with the contactless conductivity detector increasing the
Wanlu Du et al.
Cell reports, 37(3), 109848-109848 (2021-10-24)
During tumor progression, lysosome function is often maladaptively upregulated to match the high energy demand required for cancer cell hyper-proliferation and invasion. Here, we report that mucolipin TRP channel 1 (TRPML1), a lysosomal Ca2+ and Zn2+ release channel that regulates
Kevin Wiehe et al.
Cell host & microbe, 23(6), 759-765 (2018-06-05)
HIV-1 broadly neutralizing antibodies (bnAbs) require high levels of activation-induced cytidine deaminase (AID)-catalyzed somatic mutations for optimal neutralization potency. Probable mutations occur at sites of frequent AID activity, while improbable mutations occur where AID activity is infrequent. One bottleneck for induction of
Alberto Escudero et al.
Nanoscale, 8(24), 12221-12236 (2016-06-03)
Near-ultraviolet and visible excitable Eu- and Bi-doped NPs based on rare earth vanadates (REVO4, RE = Y, Gd) have been synthesized by a facile route from appropriate RE precursors, europium and bismuth nitrate, and sodium orthovanadate, by homogeneous precipitation in
Yasir S Elhassan et al.
Cell reports, 28(7), 1717-1728 (2019-08-15)
Nicotinamide adenine dinucleotide (NAD+) is modulated by conditions of metabolic stress and has been reported to decline with aging in preclinical models, but human data are sparse. Nicotinamide riboside (NR) supplementation ameliorates metabolic dysfunction in rodents. We aimed to establish
Minsoo Kim et al.
Bio-protocol, 7(14), e2405-e2405 (2017-07-20)
It is well-established that plants are able to acclimate to temperatures above or below the optimal temperature for their growth. Here, we provide protocols for assays that can be used quantitatively or qualitatively to assess the relative ability of plants
Yue-Hua Dou et al.
Electrophoresis, 23(20), 3558-3566 (2002-11-02)
Separation and direct detection of amino acids, glucose and peptide in a 3.1 cm separation channel made of poly(dimethylsiloxane) (PDMS) with end-column amperometric detection at a copper microdisk electrode was developed. This system is the integration of a normal sized
Tongqing Zhou et al.
Cell host & microbe, 28(6), 867-879 (2020-12-04)
The SARS-CoV-2 spike employs mobile receptor-binding domains (RBDs) to engage the human ACE2 receptor and to facilitate virus entry, which can occur through low-pH-endosomal pathways. To understand how ACE2 binding and low pH affect spike conformation, we determined cryo-electron microscopy
Mathieu Cayla et al.
PLoS pathogens, 10(9), e1004347-e1004347 (2014-09-19)
Protozoan pathogens of the genus Leishmania have evolved unique signaling mechanisms that can sense changes in the host environment and trigger adaptive stage differentiation essential for host cell infection. The signaling mechanisms underlying parasite development remain largely elusive even though
"(Un) suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions-a review.
Ferreira, Carlos MH, et al.
Royal Society of Chemistry Advances, 5 (39), 30989-31003 (2015)
Good, Norman E. et al.
Biochemistry, 5, 467- 477 (1966)
Kefang Xie et al.
Acta crystallographica. Section D, Biological crystallography, 61(Pt 10), 1386-1394 (2005-10-06)
The NNA7 Fab antibody fragment recognizes the human N-type blood-group antigen comprised of the N-terminal glycopeptide of glycophorin A (GPA). A mutant form of this Fab fragment, NNA7-G91S, exhibits markedly reduced antigen binding. To provide insight into how these Fab
Davide Gambarotto et al.
Nature methods, 16(1), 71-74 (2018-12-19)
Determining the structure and composition of macromolecular assemblies is a major challenge in biology. Here we describe ultrastructure expansion microscopy (U-ExM), an extension of expansion microscopy that allows the visualization of preserved ultrastructures by optical microscopy. This method allows for
Page 1 of 4