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Showing 1-30 of 58 results for "MAB1691" within Papers
Sarah E Lang et al.
Data in brief, 15, 562-566 (2017-10-27)
Secondary phosphorylation develops in myocytes expressing phospho-mimetic cardiac troponin I (cTnI) but it is not known whether multiple substitutions (e.g. cTnISDTD and cTnIS4D) cause preferential phosphorylation of the remaining endogenous or the phospho-mimetic cTnI in intact myocytes. Western analysis was
Tzila Davidov et al.
International journal of molecular sciences, 22(21) (2021-11-14)
Porcine extracellular matrix (pECM)-derived hydrogels were introduced, in recent years, aiming to benefit the pECM's microstructure and bioactivity, while controlling the biomaterial's physical and mechanical properties. The use of pECM from different tissues, however, offers tissue-specific features that can better
Tomoyuki Takahashi et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 14(5), 673-683 (2006-08-15)
The technical limitations of isolating target cells have restricted the utility of pluripotent embryonic stem (ES) cells. For example, early cardiac (i.e., precontractile) cells have not been isolated from ES cells. Here, we find that direct expression of reporter genes
Phong D Nguyen et al.
American journal of physiology. Cell physiology, 303(12), C1220-C1228 (2012-08-31)
Long-term culture of primary neonatal rat cardiomyocytes is limited by the loss of spontaneous contractile phenotype within weeks in culture. This may be due to loss of contractile cardiomyocytes from the culture or overgrowth of the non-cardiomyocyte population. Using the
Sarah E Lang et al.
Archives of biochemistry and biophysics, 601, 42-47 (2016-02-13)
A phospho-null Ala substitution at protein kinase C (PKC)-targeted cardiac troponin I (cTnI) S43/45 reduces myocyte and cardiac contractile function. The goal of the current study was to test whether cTnIS43/45N is an alternative, functionally conservative substitution in cardiac myocytes.
Christopher Jackman et al.
Acta biomaterialia, 78, 98-110 (2018-08-08)
The field of cardiac tissue engineering has developed rapidly, but structural and functional immaturity of engineered heart tissues hinder their widespread use. Here, we show that a combination of low-rate (0.2 Hz) contractile activity and thyroid hormone (T3) supplementation significantly promote
Sarah E Lang et al.
Archives of biochemistry and biophysics, 627, 1-9 (2017-06-08)
Increased protein kinase C (PKC) activity is associated with heart failure, and can target multiple cardiac troponin I (cTnI) residues in myocytes, including S23/24, S43/45 and T144. In earlier studies, cTnI-S43D and/or -S45D augmented S23/24 and T144 phosphorylation, which suggested
Robust pluripotent stem cell expansion and cardiomyocyte differentiation via geometric patterning.
Myers, FB; Silver, JS; Zhuge, Y; Beygui, RE; Zarins, CK; Lee, LP; Abilez, OJ
Integrative Biology : Quantitative Biosciences from Nano to Macro null
Sonia R Singh et al.
Autophagy, 17(10), 3124-3139 (2020-12-01)
The ubiquitin-proteasome system (UPS) and autophagy-lysosomal pathway (ALP) are two major protein degradation pathways in eukaryotic cells. Initially considered as two independent pathways, there is emerging evidence that they can work in concert. As alterations of UPS and ALP function
Manuela Mura et al.
Stem cell research, 37, 101437-101437 (2019-04-23)
We generated human induced pluripotent stem cells (hiPSCs) from dermal fibroblasts of a woman carrier of the heterozygous mutation c.568C > T p.R190W on the KCNQ1 gene. hiPSCs, obtained using four retroviruses enconding the reprogramming factors OCT4, SOX2, cMYC and KLF4, display
Laurin M Hanft et al.
American journal of physiology. Heart and circulatory physiology, 313(1), H103-H113 (2017-04-30)
Heart failure arises, in part, from a constellation of changes in cardiac myocytes including remodeling, energetics, Ca2+ handling, and myofibrillar function. However, little is known about the changes in myofibrillar contractile properties during the progression from hypertension to decompensated heart
Differential expression of genes and proteins between electric organ and skeletal muscle in the mormyrid electric fish Brienomyrus brachyistius.
Gallant, JR; Hopkins, CD; Deitcher, DL
The Journal of Experimental Biology null
Myofilament incorporation and contractile function after gene transfer of cardiac troponin I Ser43/45Ala.
Lang, SE; Robinson, DA; Wu, HC; Herron, TJ; Wahr, PA; Westfall, MV
Archives of Biochemistry and Biophysics null
Upregulations of Gata4 and oxytocin receptor are important in cardiomyocyte differentiation processes of P19CL6 cells.
Shizuka Uchida, Satoshi Fuke, Toshifumi Tsukahara
Journal of Cellular Biochemistry null
Calcitriol modulation of cardiac contractile performance via protein kinase C.
Green, John J, et al.
Journal of Molecular and Cellular Cardiology, 41, 350-359 (2006)
Cardiac muscle ring finger-1 increases susceptibility to heart failure in vivo.
Willis, MS; Schisler, JC; Li, L; Rodriguez, JE; Hilliard, EG; Charles, PC; Patterson, C
Circulation Research null
pH-responsive titratable inotropic performance of histidine-modified cardiac troponin I.
Palpant, NJ; Houang, EM; Sham, YY; Metzger, JM
Biophysical Journal null
D E Michele et al.
The Journal of cell biology, 145(7), 1483-1495 (1999-06-29)
Sarcomere maintenance, the continual process of replacement of contractile proteins of the myofilament lattice with newly synthesized proteins, in fully differentiated contractile cells is not well understood. Adenoviral-mediated gene transfer of epitope-tagged tropomyosin (Tm) and troponin I (TnI) into adult
Z Papp et al.
Cardiovascular research, 45(4), 981-993 (2000-03-23)
The involvement of Calpain-I mediated proteolysis has been implicated in myofibrillar dysfunction of reperfused myocardium following ischemia (stunning). This study addresses the question whether ultrastructural alterations might be responsible for the depressed contractility. Mechanical properties and protein composition of isolated
Audrey N Chang et al.
The Journal of biological chemistry, 290(17), 10703-10716 (2015-03-04)
In beating hearts, phosphorylation of myosin regulatory light chain (RLC) at a single site to 0.45 mol of phosphate/mol by cardiac myosin light chain kinase (cMLCK) increases Ca(2+) sensitivity of myofilament contraction necessary for normal cardiac performance. Reduction of RLC
Mitochondrial dysfunction and apoptosis underlie the pathogenic process in alpha-B-crystallin desmin-related cardiomyopathy.
Maloyan, A; Sanbe, A; Osinska, H; Westfall, M; Robinson, D; Imahashi, K; Murphy, E; Robbins, J
Circulation null
Idit Goldfracht et al.
Acta biomaterialia, 92, 145-159 (2019-05-11)
Cardiac tissue engineering provides unique opportunities for cardiovascular disease modeling, drug testing, and regenerative medicine applications. To recapitulate human heart tissue, we combined human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) with a chitosan-enhanced extracellular-matrix (ECM) hydrogel, derived from decellularized pig
Interruption of CryAB-amyloid oligomer formation by HSP22.
Sanbe, A; Yamauchi, J; Miyamoto, Y; Fujiwara, Y; Murabe, M; Tanoue, A
The Journal of Biological Chemistry null
Shohei Wakao et al.
Cellular and molecular life sciences : CMLS, 79(11), 542-542 (2022-10-07)
Stem cells undergo cytokine-driven differentiation, but this process often takes longer than several weeks to complete. A novel mechanism for somatic stem cell differentiation via phagocytosing 'model cells' (apoptotic differentiated cells) was found to require only a short time frame.
M A Moses et al.
Proceedings of the National Academy of Sciences of the United States of America, 96(6), 2645-2650 (1999-03-17)
Cartilage is an avascular and relatively tumor-resistant tissue. Work from a number of laboratories, including our own, has demonstrated that cartilage is an enriched source of endogenous inhibitors of angiogenesis. In the course of a study designed to identify novel
Qinghang Meng et al.
Circulation research, 123(12), 1285-1297 (2018-12-20)
Hypertrophic cardiomyopathy occurs with a frequency of about 1 in 500 people. Approximately 30% of those affected carry mutations within the gene encoding cMyBP-C (cardiac myosin binding protein C). Cardiac stress, as well as cMyBP-C mutations, can trigger production of
Christine Schramm et al.
The Journal of biological chemistry, 288(25), 18335-18344 (2013-05-16)
In LEOPARD syndrome (LS) patients, mutations in the protein tyrosine phosphatase Shp2 cause hypertrophic cardiomyopathy. The prohypertrophic effects of mutant Shp2 are mediated downstream by hyperactivation of mammalian target of rapamycin. Our goal was to further define the signaling cascade
Matrix identity and tractional forces influence indirect cardiac reprogramming.
Kong, YP; Carrion, B; Singh, RK; Putnam, AJ
Scientific Reports null
Rajneesh Jha et al.
Methods in molecular biology (Clifton, N.J.), 1299, 115-131 (2015-04-04)
Human pluripotent stem cells have tremendous replicative capacity and demonstrated potential to generate functional cardiomyocytes. These cardiomyocytes represent a promising source for cell replacement therapy to treat heart disease and may serve as a useful tool for drug discovery and
Suzanne E Berry et al.
Stem cells translational medicine, 2(11), 848-861 (2013-09-27)
Nestin(+) cardiac stem cells differentiate into striated cells following myocardial infarct. Transplantation of exogenous stem cells into myocardium of a murine model for Duchenne muscular dystrophy (DMD) increased proliferation of endogenous nestin(+) stem cells and resulted in the appearance of
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