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microfluidic devices for cell culture

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Showing 1-30 of 1993 results for "microfluidic devices for cell culture" within Papers
Shu-Xia Fu et al.
Biotechnology journal, 16(2), e2000126-e2000126 (2021-01-19)
Paper is increasingly recognized as a portable substrate for cell culture, due to its low-cost, flexible, and special porous property, which provides a native cellular 3D microenvironment. Therefore, paper-based microfluidics has been developed for cell culture and biomedical analysis. However
Roman G Szafran et al.
Molecules (Basel, Switzerland), 26(11) (2021-06-03)
To study the simultaneous effect of the molecular gradient of polyphenols (curcumin, trans-resveratrol, and wogonin) and biological factors released from tumor cells on apoptosis of adjacent cells, a novel microfluidic system was designed and manufactured. The small height/volume of microfluidic
Rhys J Macown et al.
Biotechnology journal, 9(6), 805-813 (2014-03-29)
The commercial use of stem cells continues to be constrained by the difficulty and high cost of developing efficient and reliable production protocols. The use of microfabricated systems combines good control over the cellular microenvironment with reduced use of resources
Andrew P Aijian et al.
Journal of laboratory automation, 20(3), 283-295 (2014-12-17)
Cell spheroids are multicellular aggregates, grown in vitro, that mimic the three-dimensional morphology of physiological tissues. Although there are numerous benefits to using spheroids in cell-based assays, the adoption of spheroids in routine biomedical research has been limited, in part
Roman G Szafran et al.
Molecules (Basel, Switzerland), 26(21) (2021-11-14)
In this study, we thoroughly analyzed molecular gradient generation, its stability over time, and linearity in our high-throughput drug screening microfluidic assay (HTS). These parameters greatly affect the precision and accuracy of the device's analytical protocol. As part of the
Alexandre Super et al.
Biotechnology journal, 11(9), 1179-1189 (2016-05-24)
Oxygen plays a key role in stem cell biology as a signaling molecule and as an indicator of cell energy metabolism. Quantification of cellular oxygen kinetics, i.e. the determination of specific oxygen uptake rates (sOURs), is routinely used to understand
Hyunryul Ryu et al.
Journal of laboratory automation, 20(3), 296-301 (2014-12-24)
The blood circulatory system links all organs from one to another to support and maintain each organ's functions consistently. Therefore, blood vessels have been considered as a vital unit. Engineering perfusable functional blood vessels in vitro has been challenging due
Kenta Shinha et al.
Micromachines, 11(7) (2020-07-28)
Numerous in vitro studies have been conducted in conventional static cell culture systems. However, most of the results represent an average response from a population of cells regardless of their local microenvironment. A microfluidic probe is a non-contact technology that
Marcel Reichen et al.
Journal of laboratory automation, 18(6), 519-529 (2013-08-24)
We present a multiplexed platform for a microfabricated stem cell culture device. The modular platform contains all the components to control stem cell culture conditions in an automated fashion. It does not require an incubator during perfusion culture and can
Taku Matsumura et al.
Biochemical and biophysical research communications, 453(1), 131-137 (2014-09-30)
The microenvironment of cells, which includes basement proteins, shear stress, and extracellular stimuli, should be taken into consideration when examining physiological cell behavior. Although microfluidic devices allow cellular responses to be analyzed with ease at the single-cell level, few have
Kirk Mutafopulos et al.
Lab on a chip, 20(21), 3914-3921 (2020-09-24)
We generate droplets in a microfluidic device using a traveling surface acoustic wave (TSAW), and control droplet size by adjusting TSAW power and duration. We combine droplet production and fluorescence detection to selectively-encapsulate cells and beads; with this active method
Jeremy Keys et al.
Methods in molecular biology (Clifton, N.J.), 1840, 101-118 (2018-08-25)
Cells migrating in tissues must often pass through physical barriers in their surroundings in the form of fibrous extracellular matrix or other cells. To improve our understanding of how cells move in such confined microenvironments, we have designed a microfluidic
Kristina Woodruff et al.
Scientific reports, 6, 23937-23937 (2016-04-01)
Mammalian synthetic biology could be augmented through the development of high-throughput microfluidic systems that integrate cellular transfection, culturing, and imaging. We created a microfluidic chip that cultures cells and implements 280 independent transfections at up to 99% efficiency. The chip
Sebastiaan J Trietsch et al.
Nature communications, 8(1), 262-262 (2017-08-16)
In vitro models that better reflect in vivo epithelial barrier (patho-)physiology are urgently required to predict adverse drug effects. Here we introduce extracellular matrix-supported intestinal tubules in perfused microfluidic devices, exhibiting tissue polarization and transporter expression. Forty leak-tight tubules are
María Virumbrales-Muñoz et al.
Molecules (Basel, Switzerland), 24(23) (2019-12-06)
Luminal geometries are common structures in biology, which are challenging to mimic using conventional in vitro techniques based on the use of Petri dishes. In this context, microfluidic systems can mimic the lumen geometry, enabling a large variety of studies.
Sanjin Hosic et al.
ACS biomaterials science & engineering, 7(7), 2949-2963 (2021-07-20)
Microfluidic organs-on-chips aim to realize more biorelevant in vitro experiments compared to traditional two-dimensional (2D) static cell culture. Often such devices are fabricated via poly(dimethylsiloxane) (PDMS) soft lithography, which offers benefits (e.g., high feature resolution) along with drawbacks (e.g., prototyping
M Sneha Maria et al.
Biomedical microdevices, 17(6), 115-115 (2015-11-14)
This work presents design, fabrication and test of a microfluidic device which employs Fahraeus-Lindqvist and Zweifach-Fung effects for cell concentration and blood cell-plasma separation. The device design comprises a straight main channel with a series of branched channels placed symmetrically
Neha Saxena et al.
STAR protocols, 2(1), 100310-100310 (2021-02-09)
In vivo cell migration is influenced by soluble factors as well as stiffness. Current in vitro strategies mostly account for one of these two factors to study cell migration. To understand the combinatorial effect of stiffness and chemokines on cell behavior
Zitong Yu et al.
STAR protocols, 4(1), 102122-102122 (2023-03-03)
Organs-on-chips are microfluidic devices for cell culturing to simulate tissue- or organ-level physiology, providing new solutions other than traditional animal tests. Here, we describe a microfluidic platform consisting of human corneal cells and compartmentalizing channels to achieve fully integrated human
David Barata et al.
Biomedical microdevices, 19(4), 81-81 (2017-09-09)
Microfluidics, the science of engineering fluid streams at the micrometer scale, offers unique tools for creating and controlling gradients of soluble compounds. Gradient generation can be used to recreate complex physiological microenvironments, but is also useful for screening purposes. For
Rsituko Ohtani-Kaneko et al.
Biomedical microdevices, 19(4), 91-91 (2017-10-11)
Induced pluripotent stem cell-derived endothelial cells (iPSC-ECs) can contribute to elucidating the pathogenesis of heart and vascular diseases and developing their treatments. Their precise characteristics in fluid flow however remain unclear. Therefore, the aim of the present study is to
Stefan Schneider et al.
Lab on a chip, 21(20), 3963-3978 (2021-10-13)
Organ-on-chip (OoC) systems have become a promising tool for personalized medicine and drug development with advantages over conventional animal models and cell assays. However, the utility of OoCs in industrial settings is still limited, as external pumps and tubing for
Ting-Ru Lin et al.
Micromachines, 10(6) (2019-06-19)
This paper reports a biomimetic microfluidic device capable of reconstituting physiological physical microenvironments in lungs during fetal development for cell culture. The device integrates controllability of both hydrostatic pressure and cyclic substrate deformation within a single chip to better mimic
Yizhong Liu et al.
Toxicology, 445, 152601-152601 (2020-09-28)
Angiogenesis is a complex process that is required for development and tissue regeneration and it may be affected by many pathological conditions. Chemicals and drugs can impact formation and maintenance of the vascular networks; these effects may be both desirable
Mathias Busek et al.
Lab on a chip, 23(4), 591-608 (2023-01-20)
We developed a novel, pump-less directional flow recirculating organ-on-a-chip (rOoC) platform that creates controlled unidirectional gravity-driven flow by a combination of a 3D-tilting system and an optimized microfluidic layout. The rOoC platform was assembled utilizing a layer-to-layer fabrication technology based
Seon Min Ju et al.
Journal of toxicology and environmental health. Part A, 78(16), 1063-1072 (2015-08-05)
A lab-on-a-chip (LOC) is a microfluidic device (MFD) that integrates several lab functions into a single chip of only millimeters in size. LOC provides several advantages, such as low fluidic volumes consumption, faster analysis, compactness, and massive parallelization. These properties
Mitsuru Komeya et al.
Scientific reports, 6, 21472-21472 (2016-02-20)
In contrast to cell cultures, particularly to cell lines, tissues or organs removed from the body cannot be maintained for long in any culture conditions. Although it is apparent that in vivo regional homeostasis is facilitated by the microvascular system
Jose M Ayuso et al.
PloS one, 10(10), e0139515-e0139515 (2015-10-09)
We report the first application of a microfluidic device to observe chemotactic migration in multicellular spheroids. A microfluidic device was designed comprising a central microchamber and two lateral channels through which reagents can be introduced. Multicellular spheroids were embedded in
Cynthia Hajal et al.
Nature protocols, 17(1), 95-128 (2022-01-09)
The blood-brain barrier (BBB) greatly restricts the entry of biological and engineered therapeutic molecules into the brain. Due to challenges in translating results from animal models to the clinic, relevant in vitro human BBB models are needed to assess pathophysiological
Somin Lee et al.
Biotechnology and bioengineering, 117(3), 748-762 (2019-11-12)
The human central nervous system (CNS) vasculature expresses a distinctive barrier phenotype, the blood-brain barrier (BBB). As the BBB contributes to low efficiency in CNS pharmacotherapy by restricting drug transport, the development of an in vitro human BBB model has
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