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Showing 1-30 of 4624 results for "mouse embryonic fibroblasts" within Papers
Leonardo Gatticchi et al.
Biochimica et biophysica acta. Molecular cell research, 1868(1), 118897-118897 (2020-10-31)
Adipogenesis is a finely orchestrated program involving a transcriptional cascade coordinated by CEBP and PPAR family members and by hormonally induced signaling pathways. Alterations in any of these factors result into impaired formation of fully differentiated adipocytes. Tm7sf2 gene encodes
Emre Balbasi et al.
Methods in molecular biology (Clifton, N.J.), 2520, 275-294 (2021-10-19)
With their unique capabilities of self-renewal and differentiation into three germ layers, mouse embryonic stem cells (mESCs) are widely used as an in vitro cellular model for early mammalian developmental studies. mESCs are traditionally cultured in high-serum and LIF-containing medium
Areta M Czerwinska et al.
Cell cycle (Georgetown, Tex.), 15(21), 2931-2942 (2016-11-02)
The transcription factor Pax7 plays a key role during embryonic myogenesis and in adult organisms in that it sustains the proper function of satellite cells, which serve as adult skeletal muscle stem cells. Recently we have shown that lack of
Carol C L Chen et al.
Genome research, 28(1), 37-51 (2017-12-13)
Phosphorylation of histone H3 at serine 10 (H3S10ph) by Aurora kinases plays an important role in mitosis; however, H3S10ph also marks regulatory regions of inducible genes in interphase mammalian cells, implicating mitosis-independent functions. Using the fluorescent ubiquitin-mediated cell cycle indicator
Yinfei Tan et al.
Journal of cellular physiology, 231(5), 1142-1150 (2015-10-08)
Although Appl1 and Appl2 have been implicated in multiple cellular activities, we and others have found that Appl1 is dispensable for mouse embryonic development, suggesting that Appl2 can substitute for Appl1 during development. To address this possibility, we generated conditionally
Hongran Wang et al.
Cellular reprogramming, 20(1), 27-37 (2018-02-08)
p53 is a barrier to somatic cell reprogramming. Deletion or transient suppression of p53 increases the efficiency of reprogramming of somatic cells into induced pluripotent stem cells. Whether p53 represents an obstacle to a similar process transdifferentiation of somatic cells
Yusuke Matsuno et al.
Scientific reports, 10(1), 5388-5388 (2020-03-28)
Genomic destabilisation is associated with the induction of mutations, including those in cancer-driver genes, and subsequent clonal evolution of cells with abrogated defence systems. Such mutations are not induced when genome stability is maintained; however, the mechanisms involved in genome
Kei Fukuda et al.
Communications biology, 4(1), 571-571 (2021-05-15)
Histone H3 lysine 9 dimethylation (H3K9me2) is a highly conserved silencing epigenetic mark. Chromatin marked with H3K9me2 forms large domains in mammalian cells and overlaps well with lamina-associated domains and the B compartment defined by Hi-C. However, the role of
Yuan Fang et al.
Journal of cellular physiology, 235(4), 3558-3568 (2019-10-09)
Fusion of differentiated somatic cells with pluripotent stem cells can be used for cellular reprogramming, but the efficiency to obtain hybrid cells is extremely low. Here, we explored a novel cell fusion system, termed single-cell fusion, the efficiency was significantly
Lauren Michelle Walker et al.
International journal of molecular sciences, 22(15) (2021-08-08)
To prevent congenital defects arising from maternal exposure, safety regulations require pre-market developmental toxicity screens for industrial chemicals and pharmaceuticals. Traditional embryotoxicity approaches depend heavily on the use of low-throughput animal models which may not adequately predict human risk. The
Dhruv Raina et al.
Development (Cambridge, England), 148(21) (2021-10-16)
During embryonic development and tissue homeostasis, reproducible proportions of differentiated cell types are specified from populations of multipotent precursor cells. Molecular mechanisms that enable both robust cell-type proportioning despite variable initial conditions in the precursor cells, and the re-establishment of
Yaqin Du et al.
Protein & cell, 10(1), 31-42 (2018-06-28)
Recently we have established a new culture condition enabling the derivation of extended pluripotent stem (EPS) cells, which, compared to conventional pluripotent stem cells, possess superior developmental potential and germline competence. However, it remains unclear whether this condition permits derivation
Megumi Tsuchiya et al.
FEBS letters, 590(16), 2671-2680 (2016-06-19)
Novel methods that increase the efficiency of gene delivery to cells will have many useful applications. Here, we report a simple approach involving depletion of p62/SQSTM1 to enhance the efficiency of gene delivery. The efficiency of reporter gene delivery was
Masaki Hosogane et al.
Genes to cells : devoted to molecular & cellular mechanisms, 22(4), 360-375 (2017-03-16)
Geminin is implicated in regulation of the cell cycle and differentiation. Although loss of Geminin triggers unscheduled DNA rereplication as a result of interruption of its interaction with Cdt1 in some somatic cancer cells, whether such cell cycle regulation also
Rachel E Turn et al.
Molecular biology of the cell, 31(18), 2070-2091 (2020-07-03)
ELMOD2 is a ∼32 kDa protein first purified by its GTPase-activating protein (GAP) activity toward ARL2 and later shown to have uniquely broad specificity toward ARF family GTPases in in vitro assays. To begin the task of defining its functions
Tetsuya S Tanaka
Methods in molecular biology (Clifton, N.J.), 1341, 295-319 (2015-04-13)
This chapter describes standard techniques to (1) maintain mouse embryonic stem cell culture, (2) deliver transgenes into mouse embryonic stem cells mediated by electroporation, nucleofection, lipofection, and retro/lentiviruses, and (3) assess the pluripotency of mouse embryonic stem cells. The last
S A Antonov et al.
Bulletin of experimental biology and medicine, 162(5), 679-683 (2017-04-02)
The cholinergic, GABAergic, and catecholaminergic neurons derived from mouse embryonic stem cells in a culture medium supplemented with recombinant NGF were phenotyped and scored. NGF stimulated generation of neurons, but not neuronal progenitors from embryonic stem cells and affected the
Christophe Papin et al.
Genome research, 27(6), 934-946 (2017-03-30)
DNA methylation is an essential epigenetic modification, present in both unique DNA sequences and repetitive elements, but its exact function in repetitive elements remains obscure. Here, we describe the genome-wide comparative analysis of the 5mC, 5hmC, 5fC, and 5caC profiles
Kun Liu et al.
Autophagy, 12(11), 2000-2008 (2016-11-02)
Pluripotent stem cells, including induced pluripotent and embryonic stem cells (ESCs), have less developed mitochondria than somatic cells and, therefore, rely more heavily on glycolysis for energy production. 1-3 However, how mitochondrial homeostasis matches the demands of nuclear reprogramming and
Muhammad Mushtaq et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(27), 15673-15683 (2020-06-24)
Stemness encompasses the capability of a cell for self-renewal and differentiation. The stem cell maintains a balance between proliferation, quiescence, and regeneration via interactions with the microenvironment. Previously, we showed that ectopic expression of the mitochondrial ribosomal protein S18-2 (MRPS18-2)
Renpeng Guo et al.
Nature communications, 9(1), 2620-2620 (2018-07-07)
Feeder cells like mouse embryonic fibroblasts (MEFs) have been widely applied for culture of pluripotent stem cells, but their roles remain elusive. Noticeably, ESCs cultured on the feeders display transcriptional heterogeneity. We investigated roles of feeder cells by examining the
Sheel Shah et al.
Cell, 174(2), 363-376 (2018-06-12)
Visualization of the transcriptome and the nuclear organization in situ has been challenging for single-cell analysis. Here, we demonstrate a multiplexed single-molecule in situ method, intron seqFISH, that allows imaging of 10,421 genes at their nascent transcription active sites in
Sophie M Morgani et al.
Developmental biology, 441(1), 104-126 (2018-07-03)
The FGF/ERK signaling pathway is highly conserved throughout evolution and plays fundamental roles during embryonic development and in adult organisms. While a plethora of expression data exists for ligands, receptors and pathway regulators, we know little about the spatial organization
Dhruv Raina et al.
Development (Cambridge, England), 149(4) (2022-02-18)
Signal transduction networks generate characteristic dynamic activities to process extracellular signals and guide cell fate decisions such as to divide or differentiate. The differentiation of pluripotent cells is controlled by FGF/ERK signaling. However, only a few studies have addressed the
Audrey Laurent et al.
PloS one, 10(4), e0122518-e0122518 (2015-04-16)
The Prep1 (Pknox1) homeodomain transcription factor is essential at multiple stages of embryo development. In the E11.5 embryo trunk, we previously estimated that Prep1 binds about 3,300 genomic sites at a highly specific decameric consensus sequence, mainly governing basal cellular
Magdalena Rohde et al.
Experimental cell research, 348(2), 123-131 (2016-10-25)
Cell migration is a central process in the development and maintenance of multicellular organisms. Tissue formation during embryonic development, wound healing, immune responses and invasive tumors all require the orchestrated movement of cells to specific locations. Histone demethylase proteins alter
Yuta Ogasawara et al.
Nature communications, 11(1), 4480-4480 (2020-09-10)
Macroautophagy initiates by formation of isolation membranes, but the source of phospholipids for the membrane biogenesis remains elusive. Here, we show that autophagic membranes incorporate newly synthesized phosphatidylcholine, and that CTP:phosphocholine cytidylyltransferase β3 (CCTβ3), an isoform of the rate-limiting enzyme
Claire S Simon et al.
Developmental cell, 55(3), 341-353 (2020-10-23)
FGF/ERK signaling is crucial for the patterning and proliferation of cell lineages that comprise the mouse blastocyst. However, ERK signaling dynamics have never been directly visualized in live embryos. To address whether differential signaling is associated with particular cell fates
Yihuan Mao et al.
Cell proliferation, 54(8), e13090-e13090 (2021-07-02)
Derivation and maintenance of pluripotent stem cells (PSCs) generally require optimized and complex culture media, which hinders the derivation of PSCs from various species. Expression of Oct4, Sox2, Klf4, and c-Myc (OSKM) can reprogram somatic cells into induced PSCs (iPSCs)
Arvind Panday et al.
Molecular cell, 81(11), 2428-2444 (2021-04-22)
Repair pathway "choice" at stalled mammalian replication forks is an important determinant of genome stability; however, the underlying mechanisms are poorly understood. FANCM encodes a multi-domain scaffolding and motor protein that interacts with several distinct repair protein complexes at stalled
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