MilliporeSigma
Search Within
Applications
Content Type

MSQC4SIGMA

Applied Filters:
Keyword:'MSQC4SIGMA'
Showing 1-13 of 13 results for "MSQC4SIGMA" within Site Content
Antibody Drug Conjugate Mimic Enables LC-MS Method Development Without Risk
Here we show how LC and MS methods may be optimized using a non-toxic surrogate of the ADC, an “ADC-mimic”, that behaves very similarly to the Cys-linked ADC Adcetris (Seattle Genetics).
Quantification of Host Cell Proteins
Residual presence of host cell proteins (HCPs) in recombinant therapeutic products has considerable clinical safety risks associated with a potential immunological response in patients.
LC-UV-MS Method Development for Antibody Drug Conjugates Using a Non-Toxic ADC-Mimic
SigmaMab Antibody Drug Conjugate Mimic, is a non-toxic drug mimic utilized as a standard for mass spectrometry and high performance liquid chromatography.
Monoclonal Antibody Titer Determination Using a Chromolith® WP 300 Protein A Wide Pore Monolithic Silica HPLC Column
Method development for titer determination of three different monoclonal antibodies - cetuximab, trastuzumab, and universal antibody standard using a Chromolith® WP 300 Protein A column.
Middle-Up Analysis of Antibodies by Reversed-Phase Chromatography
Comparative analysis of different columns in resolving medium-sized fragments of monoclonal antibodies, after digestion using dithiothreitol (DTT) or IdeS (a protease), by Reversed-Phase Chromatography.
BIOshell™ IgG 1000 Å C4 UHPLC Column for Improved Biomacromolecule Separations
BIOshell™ IgG 1000 Å C4 UHPLC Column for Improved Biomacromolecule Separations
A Rapid, Streamlined Workflow for Glycan Sample Preparation and Analysis by LC-MS
The use of PNGase Fast denaturing buffer and enzyme yielded results similar to a conventional 20-hour protocol with overnight digest while reducing workflow time to about 1 hour with a 15-minute digest.
Intact Mass Analysis of Monoclonal Antibodies
Discover our wide variety of products for intact mass analysis of monoclonal antibodies, including size-exclusion columns (SEC), ion exchange columns, reverse-phase columns, HPLC buffers, MALDI matrices and standards, high-purity solvents, reagents, tools for protein sample preparation, and certified reference materials.
Middle Up Mass Analysis Using Trastuzumab as Model Protein
A RPLC-MS workflow for mass analysis of reduced monoclonal antibody, trastuzumab, including reduction procedures, calibration, and system suitability tests.
Biosimilar Comparability Workflow for Intact Mass Analysis
A RPLC-MS workflow for mass analysis of non-reduced monoclonal antibody, trastuzumab, including calibration, and system suitability tests.
Three Methods for Critical Quality Attribute Determination of Monoclonal Antibodies
Step-by-step workflows for the intact mass analysis, peptide mapping, and N-glycan analysis of the monoclonal antibody― adalimumab, for an accurate characterization of the critical quality attributes (CQAs) to ensure drug safety and efficacy. Read more.
Workflow for High-Throughput Glycoprofiling of Rituximab
Step-by-step SEC-MS protocol for rapid glycoprofiling of a monoclonal antibody (mAb), consisting of antibody purification, a mAb reduction procedure, a mass spectrometer calibration, and a system suitability test.
Protocol for Purification, Optional Reduction, and SEC-MS Analysis of a Monoclonal Antibody
A complete workflow for the intact and middle-up mass analysis of reduced and non-reduced monoclonal antibodies based on SEC-MS with sample preparation by protein-A affinity clean-up.