head group:
Nonionic Gentle detergents used for solubilizing proteins while maintaining native subunit structure, enzymatic
activity, or other functions.
Anionic Strong detergents that often completely
Extran® rinse solutions for
residual detergent using the Spectroquant® Surfactants
(nonionic) Cell Test Kit.
Experimental Method: Nonionic
Surfactant Analysis
Nonionic surfactants (ethoxylates with 3
Brij C10 P5759 Nonionic 683* 0.002 ND ND
TRITON X-100 T8532 Nonionic 625* 0.2-0.9 100-155 80,000
* Designates detergent with average molecular weight based on content of the various polymers
ND =
crystallized, water soluble, nonionic
detergent similar to n-octyl-β-D-glucoside. In studies
on rhodopsin and opsin in different nonionic and
zwitterionic detergents, this detergent was superior in
solubility
) 19%
Density (g/mL – lb/gal) 9.0
Chemical Description
DETONOX contains anionic and nonionic detergent with wetting
agents and emulsifiers with phosphate chelating and sequestering
agents.
micelles. Method is
applicable to nonionic and zwitterionic detergents.
Application Note
Monitoring detergent removal
from biological samples using the
Direct Detect® spectrometer
EMD Millipore
the optical density (OD) at 560 nm for
Cationic/Nonionic Detergents or at 650 nm
for Anionic Detergents.
Results
1. Subtract the No Detergent Standard OD
value (Water in the Standard curve)
ALCOJET®
Low-Foaming Powdered Detergent
• Concentrated to save you money
• Biodegradable and readily disposable
• Nonionic, low-foaming cleaner for high performance from your cleaning machinery without
new detergents to help overcome
issues of solubility for 2-DE work [1]. In collaboration with
Dr. Rabilloud, Sigma-Aldrich has developed a detergent
sample pack that contains a variety of nonionic and
28; 322-330 (1965).
EVANS, W.P.: The solubilisation and inactivation of preservatives by
nonionic detergents. – J. Pharm. Pharmacol., 16 ; 323-331 (1964).
GIBBS, P.A.: Factors affecting the germination
Melting point: 38°C1
"Brij 58" is an ICI trademark name for polyoxyethylene
20 cetyl ether, a nonionic surfactant commonly used in
biochemical applications. On a hydrophilic-lipophilic
scale (HLB)
; 322-330.
EVANS, W.P. (1964): The solubilisation and inactivation of preservatives by nonionic detergents – J.
Pharm. Pharmacol., 16; 323-331.
GIBBS, P.A. (1964): Factors affecting the germination
Organic Carbon (% calculated w/w) 35–40%
Density (lb/gal) 8.25
Chemical Description
DETERGENT 8 is a nonionic, phosphate-free homogeneous blend
of an alkanolamine, glycol ethers and an alkoxylated
of membrane proteins and as a
blocking reagent in blotting applications.
TWEEN 20 is a nonionic detergent widely used in
biochemical applications. Sigma offers a number of
products for research
tested at 0.15 mg/ml
and is appropriate for use in cell culture applications.
TWEEN 20 is a nonionic detergent widely used in
biochemical applications. Sigma offers a number of
products for research
sensory switch to regulate
cell death,1,3,9 this interaction is promoted by the
presence of nonionic detergents, which stimulate Bax
dimer formation. Other hypotheses propose the
formation of channels
POLYOXYETHYLENE 10 OLEOYL ETHER
Sigma product number P6136
GENERAL REMARKS:
Brij detergents are nonionic surfactants generally called polyoxyethylene (POE) ethers. In general,
increasing alkyl group
analyzed for the absence of
endonuclease, exonuclease, and RNAse activities.
TWEEN 20 is a nonionic detergent widely used in
biochemical applications. Sigma offers a number of
products for research
alkaline detergent containing potassium
hydroxide, silicate of soda and sodium hypochlorite. It should be used
with caution and the precautions on the label should be followed.
DETOJET is nonionic.
Cleaning
electrophoresis.
Proteomics, 3, 111-121 (2003).
3. Luche, S., et al., Evaluation of nonionic and zwitterionic detergents as
membrane protein solubilizers in two-dimensional electrophoresis.
Proteomics, 3
containing strong cation exchange resin for the concentration
of peptides and removal of salts and nonionic detergents from
peptide mixtures prior to mass spectrometry.
Strong cation exchange (SCX) chromatography
Chemical Description
TERGAJET is a highly alkaline detergent containing sodium
carbonate, silicate of soda, and surfactants. TERGAJET is nonionic,
biodegradable, and water soluble.
Cleaning Validation
5. Rang, M. J., and Miller, C. A., Spontaneous
Emulsification of Oils Containing Hydrocarbon,
Nonionic Surfactant, and Oleyl Alcohol. J. Colloid
Interface Sci., 209(1), 179-192 (1999).
6. Vrionis
not considered to be functionally
interchangeable in most applications.
Triton X-100 is a nonionic detergent, 100% active
ingredient, which is often used in biochemical
applications to solubilize proteins
peroxides to lighten the color to its yellow-amber color.4
GENERAL REMARKS:
Tween detergents are nonionic surfactants comprised of a sorbitan ring, an alkyl group and � 20 ethylene oxide units.
Tween
Cr2O72- 5 NH4+ 1000 Zn2+ 1000
Reducing agents interfere with the determination.
1) tested with nonionic, cationic, and anionic surfactants
5. Reagents and auxiliaries
Please note the warnings on
wild type proteins. CelLytic B Reagent consists of
20 mM Tris-HCl, pH 7.5, and a proprietary nonionic
detergent. There is no need for special equipment such
as a sonicator or a French press with this kit
working dilutions by titration test.
References
1. Hsu, Y-T and Youle, R.J. Nonionic detergents
induce dimerization among members of the Bcl-2
family. J. Biol. Chem., 272, 13829-13874
Cr2O72- 25 NH4+ 1000 Zn2+ 1000
Reducing agents interfere with the determination.
1) tested with nonionic, cationic, and anionic surfactants
5. Reagents and auxiliaries
Please note the warnings on
sensory
switch to regulate cell death,1,2,6 this interaction is
promoted by the presence of nonionic detergents, which
stimulate Bax dimer formation. Other hypotheses
propose the formation of channels