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nonionic detergents
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Showing 1-30 of 251 results for "nonionic detergents" within Papers
Katarina Siposova et al.
Colloids and surfaces. B, Biointerfaces, 150, 445-455 (2016-11-16)
Two nonionic detergents, Triton X-100 (TX-100) and n-dodecyl-β-d-maltoside (DDM) were tested for their ability to affect lysozyme amyloid aggregation. We have demonstrated that fibrillization of lysozyme is completely inhibited by low sub-micellar concentrations of both of these detergents. The apparent
N A Hosea et al.
Archives of biochemistry and biophysics, 353(2), 365-373 (1998-06-02)
Nonionic phenolic detergents are commonly used in the purification of membrane-associated proteins. Triton N-101 was shown to be oxidized by NADPH-fortified human liver microsomes and recombinant human cytochromes P450 (P450). Oxidation was monitored using HPLC and the fluorescence properties of
B R Ganong et al.
Analytical biochemistry, 179(1), 66-71 (1989-05-15)
Four commercial nonionic polyoxyethylene detergents were compared with Triton X-100 in terms of physicochemical properties and interaction with an integral membrane protein, phosphatidylinositol kinase. Tergitol 15-S-9, Tergitol TMN-10, polyoxyethylene 10 lauryl ether, and polyoxyethylene 10 tridecyl ether possess aliphatic hydrophobic
Y T Hsu et al.
The Journal of biological chemistry, 272(21), 13829-13834 (1997-05-23)
Members of the Bcl-2 family (including Bcl-2, Bcl-XL, and Bax) play key roles in the regulation of apoptosis. These proteins are believed to be membrane-associated and have been proposed to regulate apoptosis through both homodimerization and heterodimerization. We have found
A Walter et al.
Biochimica et biophysica acta, 1508(1-2), 20-33 (2000-11-25)
The vesicle-to-micelle transition of egg phosphatidylcholine LUVs induced by octylglucoside was studied in buffers with 0-4 M sodium chloride, sucrose or urea. We used both light scattering and fluorescent probes to follow the lipid-detergent complexes in these buffers. The vesicle-to-micelle
G Pappert et al.
Biochimica et biophysica acta, 730(1), 32-40 (1983-04-21)
Band 3 protein, the anion transport protein of the human erythrocyte membrane, was solubilized and purified in aqueous solutions of two nonionic detergents: Ammonyx-LO (dimethyl laurylamine oxide) and C12E9 (nonaethylene glycol lauryl ether). The state of association of the purified
L M Hjelmeland et al.
Analytical biochemistry, 130(2), 485-490 (1983-04-15)
Detergents containing either a cholic acid, a deoxycholic acid, or an octanoic acid-like hydrophobic moiety and a bisgluconamidopropyl polar group were synthesized. Extinction coefficients, partial specific volumes, critical micelle concentrations, and aggregation numbers were determined for each of the detergents.
Evaluation of nonionic and zwitterionic detergents as membrane protein solubilizers in two-dimensional electrophoresis
Luche S, et al.
Proteomics, 3(3), 249-253 (2003)
A Schwarz et al.
Analytical biochemistry, 254(2), 221-225 (1998-01-07)
A rapid, simple, and efficient chloroform/methanol-free method of isolating gangliosides is described. The nonionic polyoxyethylene detergent, hexaethyleneglycol mono-n-tetradecyl ether (C14EO6), forms clear micellar solutions in water, but two-phase separation can be achieved by centrifugation in the presence of ammonium sulfate
Plasmid DNA isolation utilizing a novel nonionic detergent.
M Altschuler et al.
BioTechniques, 17(3), 434-434 (1994-09-01)
T Shimamoto et al.
Journal of biochemistry, 97(6), 1807-1810 (1985-06-01)
A new nonionic detergent, heptylthioglucoside, was synthesized and found to be more soluble than octylthioglucoside in water at low temperatures. Use of this detergent for solubilization and reconstitution of membrane proteins of Escherichia coli was examined. Heptylthioglucoside was as effective
Pitfalls in the use of commercial nonionic detergents for the solubilization of integral membrane proteins: sulfhydryl oxidizing contaminants and their elimination.
H W Chang et al.
Analytical biochemistry, 104(1), 112-117 (1980-05-01)
S Sinha et al.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 95(3), 379-384 (2017-02-09)
Shell Chemical Company Nonidet P-40 has been used for decades in many biochemical assays as a nonionic, nondenaturing detergent; however, Shell no longer manufactures this product. Four commercially available substitutes were investigated and their activities titrated in an intracellular tubulin
M Kellermayer et al.
Journal of cellular physiology, 159(2), 197-204 (1994-05-01)
The plasma membrane of erythrocytes, as of other cells, is thought to act as the barrier responsible for maintaining intracellular gradients of most ions and small molecular species between the cell and its environment. Controlled application of the nonionic detergent
Critical micelle concentration determination of nonionic detergents with Coomassie Brilliant Blue G-250
Ken S. Rosenthal and Frank Koussaie
Analytical Chemistry, 55, 1115-1115 (1983)
Cloud-point extraction of gangliosides using nonionic detergent C14EO6.
G C Terstappen et al.
Methods in enzymology, 312, 187-196 (2000-11-09)
T Tsuchiya et al.
Journal of biochemistry, 96(5), 1593-1597 (1984-11-01)
n-Octyl-beta-D-thioglucopyranoside (octylthioglucoside), a new nonionic detergent, was synthesized. Properties of this detergent and its applicability to membrane biochemistry were investigated. The critical micelle concentration of this detergent was determined to be 9 mM. Membrane proteins of Escherichia coli were effectively
Ecotoxicological effects of a nonionic detergent (Triton DF-16) assayed by modFETAX.
C Presutti et al.
Bulletin of environmental contamination and toxicology, 53(3), 405-411 (1994-09-01)
Synthesis of environment friendly nonionic surfactants from sugar base and characterization of interfacial properties for detergent application
Lee S, et al.
Journal of Industrial and Engineering Chemistry, 38(19), 157-166 (2016)
Azusa Asada et al.
Bioscience, biotechnology, and biochemistry, 75(2), 376-378 (2011-02-11)
Solubilization and structural stability of a membrane protein bacteriorhodopsin (bR) with n-octyl-β-thioglucoside (OTG) was investigated in comparison with a previous study on bR solubilized with n-octyl-β-glucoside (OG). Highly efficient and stable solubilization of bR with OTG was accomplished above the
Ivan L Budyak et al.
Journal of pharmaceutical sciences, 104(4), 1543-1547 (2014-12-30)
Robust higher order structure (HOS) characterization capability and strategy are critical throughout biopharmaceutical development from initial candidate selection and formulation screening to process optimization and manufacturing. This case study describes the utility of several orthogonal HOS methods as investigational tools
B Fricke
Analytical biochemistry, 281(2), 144-150 (2000-06-28)
Zwitterionic detergents interfere with the salt-induced phase separation for nonionic detergents in a concentration-dependent manner by shifting the normal cloud point of nonionic detergents to a higher ionic strength at room temperature. This phenomenon was used to determine the concentration
Joo-Yul Bae et al.
Neural regeneration research, 16(9), 1890-1896 (2021-01-30)
Animal studies have shown that amphoteric detergent and nuclease (DNase I and ribonuclease A) is the most reliable decellularization method of the peripheral nerve. However, the optimal combination of chemical reagents for decellularization of human nerve allograft needs further investigation.
J Lu et al.
Biochemical and biophysical research communications, 196(1), 12-17 (1993-10-15)
Kinetic analyses indicate that the inhibitory effects of the nonionic detergents Triton X-100 and Nonidet P-40 on chloramphenicol acetyltransferase are exerted by a competitive and a non-competitive mechanism with respect to the substrates chloramphenicol and acetyl-CoA, respectively. Comparison with nonionic
Young Ho Shin et al.
Journal of tissue engineering and regenerative medicine, 13(7), 1241-1252 (2019-05-06)
Little consensus exists regarding which decellularization technique best removes the cellular components while maintaining structural integrity. We aimed to identify the most efficient and safest decellularization method by combining previously established chemical (detergent based) and biological (nuclease based) methods in
Young Ho Shin et al.
Journal of tissue engineering and regenerative medicine, 13(7), 1241-1252 (2019-05-06)
Little consensus exists regarding which decellularization technique best removes the cellular components while maintaining structural integrity. We aimed to identify the most efficient and safest decellularization method by combining previously established chemical (detergent based) and biological (nuclease based) methods in
Parviz Lalezari et al.
Analytical biochemistry, 416(1), 92-99 (2011-05-25)
A new method for extraction and concentration of organic dyes that uses a reagent composed of a nonionic detergent mixed with an alcohol is described. We have observed that water-soluble organic dyes are also soluble in nonionic detergents and can
L A Lowthert et al.
Biochemical and biophysical research communications, 206(1), 370-379 (1995-01-05)
Intermediate filament (IF) proteins make up some of the most insoluble proteins known, and within the IF protein family, keratins are the least soluble. We compared the efficiency of nonionic, cationic, mixed nonionic and anionic, and zwitterionic detergents in solubilizing
A Kyaw et al.
Analytical biochemistry, 145(2), 230-234 (1985-03-01)
A new colorimetric procedure is described for inorganic phosphate determination using the color reaction between inorganic phosphate and acidified ammonium molybdate in the presence of Triton X-100. The method is simple and specific, and produces results comparable with those of
A Ambesi et al.
Analytical biochemistry, 198(2), 312-317 (1991-11-01)
Solubilization and reconstitution of the cardiac sarcolemmal Na+/Ca2+ exchanger by use of the anionic detergent cholate and its application for reconstitution of the exchanger following solubilization with zwitterionic or nonionic detergents is described. Solubilization and reconstitution with cholate provided a
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