Search Within


Applied Filters:
Showing 1-30 of 608 results for "O5500" within Papers
Sergey M Zimatkin et al.
Alcohol (Fayetteville, N.Y.), 49(6), 589-595 (2015-09-01)
The purpose of the study is to clarify the effect of 7 days of ethanol administration upon brain histaminergic neurons in rats. Male Wistar rats were injected intraperitoneally (i.p.) with 20% ethanol/saline (0.85% NaCl) daily, over 7 days, whereas control
Urszula Stachewicz et al.
Acta biomaterialia, 27, 88-100 (2015-09-09)
The interaction between resident cells and electrospun nanofibers is critical in determining resultant osteoblast proliferation and activity in orthopedic tissue scaffolds. The use of techniques to evaluate cell-nanofiber interactions is critical in understanding scaffold function, with visualization promising unparalleled access
Åsa M E Winther et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 35(44), 14756-14770 (2015-11-06)
Many endocytic proteins accumulate in the reserve pool of synaptic vesicles (SVs) in synapses and relocalize to the endocytic periactive zone during neurotransmitter release. Currently little is known about their functions outside the periactive zone. Here we show that in
Manuel Marx et al.
Nature protocols, 7(2), 394-407 (2012-02-04)
In this report, we describe a reliable protocol for biocytin labeling of neuronal tissue and diaminobenzidine (DAB)-based processing of brain slices. We describe how to embed tissues in different media and how to subsequently histochemically label the tissues for light
Annette R Rodriguez et al.
Cellular immunology, 295(2), 83-91 (2015-04-13)
The human pathogen Chlamydia pneumoniae has been implicated in chronic inflammatory diseases including type 2 diabetes. Therefore, we designed a study to evaluate pancreatic beta cells and mast cells during chlamydial infection. Our study revealed that C. pneumoniae infected mast
Eloah C D Lyrio et al.
Experimental dermatology, 24(7), 536-542 (2015-04-02)
Leprosy is a chronic granulomatous disease caused by Mycobacterium leprae affecting the skin and peripheral nerves. Despite M. leprae invasion of the skin and keratinocytes importance in innate immunity, the interaction of these cells in vitro during M. leprae infection is poorly understood.
Yan-Yan Wu et al.
Journal of economic entomology, 108(4), 1486-1494 (2015-10-16)
Honey bees are at an unavoidable risk of exposure to neonicotinoid pesticides, which are used worldwide. Compared with the well-studied roles of these pesticides in nontarget site (including midgut, ovary, or salivary glands), little has been reported in the target
York Rudhard et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 35(7), 2927-2941 (2015-02-24)
Axon degeneration is a programed process that takes place during development, in response to neuronal injury, and as a component of neurodegenerative disease pathology, yet the molecular mechanisms that drive this process remain poorly defined. In this study, we have
Michal Bartnikowski et al.
Biotechnology and bioengineering, 111(7), 1440-1451 (2014-01-30)
Tissue engineering focuses on the repair and regeneration of tissues through the use of biodegradable scaffold systems that structurally support regions of injury while recruiting and/or stimulating cell populations to rebuild the target tissue. Within bone tissue engineering, the effects
Cheng Teng Ng et al.
Anatomical record (Hoboken, N.J. : 2007), 298(2), 418-427 (2014-09-23)
Gold nanoparticles (AuNPs) have potential biomedical and scientific applications. In this study, we evaluated the uptake and internalization of FBS-coated 20 nm AuNPs into lung fibroblasts and liver cells by different microscopy techniques. AuNP aggregates were observed inside MRC5 lung
Natalie M Kofler et al.
Scientific reports, 5, 16449-16449 (2015-11-14)
Pericytes regulate vessel stability and pericyte dysfunction contributes to retinopathies, stroke, and cancer. Here we define Notch as a key regulator of pericyte function during angiogenesis. In Notch1(+/-); Notch3(-/-) mice, combined deficiency of Notch1 and Notch3 altered pericyte interaction with
Veronica Bianchi et al.
Reproductive biology and endocrinology : RB&E, 12, 110-110 (2014-11-26)
Human mature oocytes are very susceptible to cryodamage. Several reports demonstrated that vitrification might preserve oocyte better than slow freezing. However, this is still controversial. Thus, larger clinical, biological and experimental trials to confirm this concept are necessary. The aim
Shelly R H Pecorella et al.
American journal of physiology. Lung cellular and molecular physiology, 309(8), L857-L871 (2015-07-19)
The heme oxygenase-1 (HO-1)/carbon monoxide (CO) system induces mitochondrial biogenesis, but its biological impact in human skeletal muscle is uncertain. The enzyme system generates CO, which stimulates mitochondrial proliferation in normal muscle. Here we examined whether CO breathing can be
Matthia A Karreman et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 61(3), 236-247 (2012-12-25)
One of the major challenges for correlative microscopy is the preparation of the sample; the protocols for transmission electron microscopy (TEM) and fluorescence microscopy (FM) often prove to be incompatible. Here, we introduce 2+Staining: an improved contrasting procedure for Tokuyasu
Robert H J Bandsma et al.
American journal of physiology. Endocrinology and metabolism, 309(5), E466-E473 (2015-07-15)
Severe malnutrition is a leading cause of global childhood mortality, and infection and hypoglycemia or hyperglycemia are commonly present. The etiology behind the changes in glucose homeostasis is poorly understood. Here, we generated an animal model of severe malnutrition with
Oscar Rodríguez-Espinosa et al.
Immunology, 145(2), 213-224 (2014-12-30)
As part of the innate immune response, neutrophils are at the forefront of defence against infection, resolution of inflammation and wound healing. They are the most abundant leucocytes in the peripheral blood, have a short lifespan and an estimated turnover
Uttio Roy Chowdhury et al.
PloS one, 10(11), e0141783-e0141783 (2015-11-05)
Elevated intraocular pressure (IOP) is the most prevalent and only treatable risk factor for glaucoma, a leading cause of irreversible blindness worldwide. Unfortunately, all current therapeutics used to treat elevated IOP and glaucoma have significant and sometimes irreversible side effects
Stephanie A Rey et al.
Nature communications, 6, 8043-8043 (2015-08-22)
Efficient recycling of synaptic vesicles is thought to be critical for sustained information transfer at central terminals. However, the specific contribution that retrieved vesicles make to future transmission events remains unclear. Here we exploit fluorescence and time-stamped electron microscopy to
Michael J Glass et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 35(26), 9558-9567 (2015-07-03)
Hypertension induced by angiotensin II (Ang II) is associated with glutamate-dependent dysregulation of the hypothalamic paraventricular nucleus (PVN). Many forms of glutamate-dependent plasticity are mediated by NMDA receptor GluN1 subunit expression and the distribution of functional receptor to the plasma
Jessica L Forbester et al.
Infection and immunity, 83(7), 2926-2934 (2015-05-13)
The intestinal mucosa forms the first line of defense against infections mediated by enteric pathogens such as salmonellae. Here we exploited intestinal "organoids" (iHOs) generated from human induced pluripotent stem cells (hIPSCs) to explore the interaction of Salmonella enterica serovar
Pachara Sattayawat et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(3), 1404-1413 (2020-01-10)
Bio-based production technologies may complement or replace petroleum-based production of chemicals, but they face a number of technical challenges, including product toxicity and/or water insolubility. Plants and microorganisms naturally biosynthesize chemicals that often are converted into derivatives with reduced toxicity
Ling-Chao Meng et al.
Chinese medical journal, 128(21), 2902-2905 (2015-11-03)
Mutations of transthyretin (TTR) cause the most common type of autosomal-dominant hereditary systemic amyloidosis, which occurs worldwide. To date, more and more mutations in the TTR gene have been reported. Some variations in the clinical presentation are often observed in
X Zhao et al.
International journal of molecular medicine, 35(3), 684-692 (2015-01-13)
Tubular atrophy and dysfunction is a critical process underlying diabetic nephropathy (DN). Understanding the mechanisms underlying renal tubular epithelial cell survival is important for the prevention of kidney failure associated with glucotoxicity. Autophagy is a cellular pathway involved in protein
Shannon N Campbell et al.
Journal of Alzheimer's disease : JAD, 43(3), 967-976 (2014-08-16)
Clinical and basic science research suggests that stress and/or changes in central stress signaling intermediates may be involved in Alzheimer's disease (AD) pathogenesis. Although the links between stress and AD remain unsettled, data from our group and others have established
Jeanine Alterio et al.
Traffic (Copenhagen, Denmark), 16(9), 978-993 (2015-06-17)
Yif1B is an intracellular membrane-bound protein belonging to the Yip family, shown previously to control serotonin 5-HT1A receptor targeting to dendrites. Because some Yip proteins are involved in the intracellular traffic between the ER and the Golgi, here we investigated
Li-na Sun et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 68(8), 1079-1088 (2014-12-03)
Epithelial-mesenchymal transition (EMT) is recognized to play a key role in diabetic nephropathy (DN). Curcumin, the main active component of turmeric extracted from the roots of the Curcuma longa plant, has been reported for its anti-fibrotic effects in kidney fibrosis. The
Lei Jiang et al.
Cancer biology & therapy, 16(3), 466-476 (2015-03-18)
Glucocorticoid (GC) resistance remains a major obstacle to successful treatment of lymphoid malignancies. Till now, the precise mechanism of GC resistance remains unclear. In the present study, dexamethasone (Dex) inhibited cell proliferation, arrested cell cycle in G0/G1-phase, and induced apoptosis
Christopher C Wong et al.
Acta oto-laryngologica, 133(1), 22-27 (2012-09-21)
The use of both gadolinium chloride (GdCl(3)) and osmium tetroxide (OsO(4)) allowed for the visualization of the membranous labyrinth and other intralabyrinthine structures, at different intensities, as compared with the control sample. This initial comparison shows the advantages of GdCl(3)
Eirini G Velliou et al.
Bioprocess and biosystems engineering, 38(8), 1589-1600 (2015-04-26)
A great challenge when conducting ex vivo studies of leukaemia is the construction of an appropriate experimental platform that would recapitulate the bone marrow (BM) environment. Such a 3D scaffold system has been previously developed in our group [1]. Additionally
Han Zhou et al.
Molecular medicine reports, 11(6), 4381-4388 (2015-02-06)
Polyethylenimine (PEI) has been demonstrated to be an effective non‑viral synthetic polymer for efficient gene delivery amongst various cell types in vitro and in vivo. In the present study, 25 kDa linear PEI (L‑PEI) was used to transfer plasmid DNA (pDNA), encoding the
Page 1 of 21