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Showing 1-30 of 43 results for "P4390" within Papers
Katrin Heindl et al.
The EMBO journal, 29(24), 4161-4171 (2010-11-11)
In a cell, an enormous amount of energy is channelled into the biogenesis of ribosomal RNAs (rRNAs). In a multistep process involving a large variety of ribosomal and non-ribosomal proteins, mature rRNAs are generated from a long polycistronic precursor. Here
Cheng Jiang et al.
Analytica chimica acta, 766, 88-93 (2013-02-23)
T4 polynucleotide kinase (PNK) plays a critical role in various cellular events. Here, we describe a novel colorimetric strategy for estimating the activity of PNK and screening its inhibitors taking advantage of the efficient cleavage of λ exonuclease and the
Sandeep K Sharma et al.
Nature chemical biology, 6(12), 914-920 (2010-10-19)
Hsp70-Hsp40-NEF and possibly Hsp100 are the only known molecular chaperones that can use the energy of ATP to convert stably pre-aggregated polypeptides into natively refolded proteins. However, the kinetic parameters and ATP costs have remained elusive because refolding reactions have
T Iwaki et al.
Biochemistry, 32(29), 7589-7592 (1993-07-27)
We have prepared monoclonal antibodies for the fluorescent molecular rotors 9-(2-carboxy-2-cyanovinyl)julolidine (CCVJ) and 9-(dicyanovinyl)julolidine (DCVJ). Mouse monoclonal antibody (IgG2b) prepared against CCVJ-conjugated bovine serum albumin strongly bound CCVJ and DCVJ. The CCVJ (or DCVJ) binding to IgG and Fab was
Hiroyuki Furusawa et al.
The Analyst, 137(6), 1334-1337 (2012-02-03)
Formation and decomposition of the enzyme-substrate (ES) complex during phosphorylation by T4 polynucleotide kinase (T4 PNK) of dsDNAs were monitored using a highly sensitive quartz crystal microbalance (QCM) to determine kinetic parameters, which were characterised in comparison with those of
Lei Lin et al.
Analytical chemistry, 83(22), 8396-8402 (2011-10-27)
Phosphorylation of DNA with 5'-hydroxyl termini plays a critical role in a majority of normal cellular events, including DNA recombination, DNA replication, and repair of DNA during strand interruption. Determination of nucleotide kinase activity and inhibition is under intense development
Ga Tremblay et al.
Bioanalysis, 3(5), 499-508 (2011-03-11)
Oligonucleotide-based therapeutics are quantified with hybridization assays in biological matrices such as plasma and tissues. Current hybridization methods do not entirely discriminate the parent compound from 5´- or 3´-N-X truncated metabolites. A dual ligation-based hybridization assay was developed to circumvent
Polynucleotide kinase exchange reaction: quantitave assay for restriction endonuclease-generated 5'-phosphoroyl termini in DNA.
K L Berkner et al.
The Journal of biological chemistry, 252(10), 3176-3184 (1977-05-25)
Ushati Das et al.
Nucleic acids research, 42(2), 1152-1161 (2013-10-24)
Clostridium thermocellum polynucleotide kinase (CthPnk), the 5' end-healing module of a bacterial RNA repair system, catalyzes reversible phosphoryl transfer from an NTP donor to a 5'-OH polynucleotide acceptor. Here we report the crystal structures of CthPnk-D38N in a Michaelis complex
Lisa Patel et al.
Biochemical and biophysical research communications, 300(2), 472-476 (2002-12-31)
Resistin is a cysteine-rich protein postulated to be a molecular link between obesity and type 2 diabetes. The aim of this study was to investigate the role of PPAR gamma in the regulation of resistin expression in human primary macrophages.
Kyle M Burns et al.
Molecular & cellular proteomics : MCP, 12(2), 539-548 (2012-12-01)
Hydrogen-deuterium exchange mass spectrometry is an important method for protein structure-function analysis. The bottom-up approach uses protein digestion to localize deuteration to higher resolution, and the essential measurement involves centroid mass determinations on a very large set of peptides. In
Živilė Strazdaitė-Žielienė et al.
Archives of virology, 159(2), 327-331 (2013-08-21)
The bacteriophage T4 insertion-substitution (I/S) vector system has become one of the most important tools for the introduction of site-directed mutations into the T4 genome. In this study, we show that the I/S phage T4 K10 carries two point mutations
Feng Chen et al.
Biosensors & bioelectronics, 47, 218-224 (2013-04-16)
DNA phosphorylation, catalyzed by polynucleotide kinase (PNK), plays significant regulatory roles in many biological events. Herein, using T4 PNK as a model target, we describe a one-step, highly sensitive, simple and rapid fluorescence approach for monitoring its activity and inhibition.
Ushati Das et al.
Nucleic acids research, 41(1), 355-365 (2012-11-03)
T4 polynucleotide kinase-phosphatase (Pnkp) exemplifies a family of enzymes with 5'-kinase and 3'-phosphatase activities that function in nucleic acid repair. The polynucleotide 3'-phosphatase reaction is executed by the Pnkp C-terminal domain, which belongs to the DxDxT acylphosphatase superfamily. The 3'-phosphatase
Ushati Das et al.
Journal of bacteriology, 196(24), 4285-4292 (2014-10-01)
Clostridium thermocellum polynucleotide kinase (CthPnk), the 5'-end-healing module of a bacterial RNA repair system, catalyzes reversible phosphoryl transfer from a nucleoside triphosphate (NTP) donor to a 5'-OH polynucleotide acceptor, either DNA or RNA. Here we report the 1.5-Å crystal structure
Christopher D Castle et al.
Molecular biology of the cell, 23(4), 716-728 (2011-12-23)
The coordination of RNA polymerase I transcription with pre-rRNA processing, preribosomal particle assembly, and nuclear export is a finely tuned process requiring the concerted actions of a number of accessory factors. However, the exact functions of some of these proteins
Problem-solving test: restriction endonuclease mapping.
József Szeberényi
Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology, 39(5), 393-395 (2011-09-29)
Yonghong Wang et al.
Biosensors & bioelectronics, 32(1), 213-218 (2012-01-03)
A novel electrochemical strategy for monitoring the activity and inhibition of T4 polynucleotide kinase (PNK) is developed by use of titanium ion (Ti(4+)) mediated signal transition coupled with signal amplification of single wall carbon nanotubes (SWCNTs). In this method, a
Tathagata Choudhuri et al.
FEBS letters, 512(1-3), 334-340 (2002-02-20)
The aim of this study was to determine the mechanisms of curcumin-induced human breast cancer cell apoptosis. From quantitative image analysis data showing an increase in the percentage of cells with a sub-G0/G1 DNA content, we demonstrated curcumin-induced apoptosis in
Nigel J Jones
Methods in molecular biology (Clifton, N.J.), 817, 183-206 (2011-12-08)
32P-postlabelling is a technique originally described by Kurt Randerath and colleagues for the sensitive detection of damage produced in DNA by reactive chemicals or genotoxins. The procedure essentially entails the enzymatic digestion of DNA to nucleoside 3'-monophosphates which are then
Audun Hanssen-Bauer et al.
Environmental and molecular mutagenesis, 52(8), 623-635 (2011-07-26)
XRCC1 is a scaffold protein capable of interacting with several DNA repair proteins. Here we provide evidence for the presence of XRCC1 in different complexes of sizes from 200 to 1500 kDa, and we show that immunoprecipitates using XRCC1 as
Barbara S Remus et al.
RNA (New York, N.Y.), 20(4), 462-473 (2014-02-21)
Plant and fungal tRNA ligases are trifunctional enzymes that repair RNA breaks with 2',3'-cyclic-PO4 and 5'-OH ends. They are composed of cyclic phosphodiesterase (CPDase) and polynucleotide kinase domains that heal the broken ends to generate the 3'-OH, 2'-PO4, and 5'-PO4
A role in true-late gene expression for the T4 bacteriophage 5' polynucleotide kinase 3' phosphatase.
K Sirotkin et al.
Journal of molecular biology, 123(2), 221-233 (1978-08-05)
Eduardo Paredes et al.
Methods (San Diego, Calif.), 54(2), 251-259 (2011-03-01)
Advances in RNA nanotechnology will depend on the ability to manipulate, probe the structure and engineer the function of RNA with high precision. This article reviews current abilities to incorporate site-specific labels or to conjugate other useful molecules to RNA
Huping Jiao et al.
Chemical communications (Cambridge, England), 48(63), 7862-7864 (2012-07-13)
A single stranded oligonucleotide could induce aggregation of a perylene probe, the probe's monomer fluorescence was efficiently quenched. However, when the oligonucleotide was 5'-phosphorylated by polynucleotide kinase, it could be very efficiently degraded by lambda exonuclease, probe monomers were released
The enzymatic phosphorylation of ribonucleic acid and deoxyribonucleic acid. II. Further properties of the 5'-hydroxyl polynucleotide kinase.
A Novogrodsky et al.
The Journal of biological chemistry, 241(12), 2933-2943 (1966-06-25)
Ekaterina Kashkina et al.
DNA repair, 11(8), 676-683 (2012-07-04)
We previously reported that Schizosaccharomyces pombe pnk1 cells are more sensitive than wild-type cells to γ-radiation and camptothecin, indicating that Pnk1 is required for DNA repair. Here, we report that pnk1pku70 and pnk1rhp51 double mutants are more sensitive to γ-radiation
Liangliang Zhang et al.
Biosensors & bioelectronics, 44, 6-9 (2013-02-06)
Phosphorylation of DNA by polynucleotide kinase (PNK) takes an important role in DNA damage repair, replication and recombination. The evaluation of PNK activity has received an increasing attention due to the significance of PNK. Here, we present a label-free fluorescent
V Cameron et al.
Biochemistry, 16(23), 5120-5126 (1977-11-15)
The purification of T4 polynucleotide kinase results in the copurification of an activity which will specifically remove the 3'-terminal phosphate from a variety of deoxyribonucleotides and ribonucleotides in the absence of ATP. This phosphatase activity requires magnesium, has a pH
Li Kai Wang et al.
Nucleic acids research, 41(11), 5864-5873 (2013-04-19)
Pnkp is the end-healing and end-sealing component of an RNA repair system present in diverse bacteria from many phyla. Pnkp is composed of three catalytic modules: an N-terminal polynucleotide 5' kinase, a central 2',3' phosphatase and a C-terminal ligase. The
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