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Showing 1-30 of 168 results for "P4850" within Papers
N García-Romero et al.
Journal of translational medicine, 17(1), 75-75 (2019-03-16)
Extracellular vesicles (EVs) are small membrane-bound vesicles which play an important role in cell-to-cell communication. Their molecular cargo analysis is presented as a new source for biomarker detection, and it might provide an alternative to traditional solid biopsies. However, the
Catherine Arden et al.
Diabetes, 60(12), 3110-3120 (2011-10-21)
The induction of hepatic glucose 6-phosphatase (G6pc) by glucose presents a paradox of glucose-induced glucose intolerance. We tested whether glucose regulation of liver gene expression is geared toward intracellular homeostasis. The effect of glucose-induced accumulation of phosphorylated intermediates on expression
Mazène Hochane et al.
PLoS biology, 17(2), e3000152-e3000152 (2019-02-23)
The current understanding of mammalian kidney development is largely based on mouse models. Recent landmark studies revealed pervasive differences in renal embryogenesis between mouse and human. The scarcity of detailed gene expression data in humans therefore hampers a thorough understanding
Dong Woo Shin et al.
Clinical lymphoma, myeloma & leukemia, 19(8), e496-e505 (2019-06-22)
Little is known about the mutational frequency of myeloid differentiation factor 88 (MYD88) and C-X-C chemokine receptor type 4 (CXCR4) and the corresponding characteristics in Asian individuals afflicted with Waldenström macroglobulinemia (WM). We investigated the characteristics of WM according to
Gerhild Zauner et al.
Journal of proteome research, 11(12), 5804-5814 (2012-10-12)
Human fibrinogen is a 340 kDa, soluble plasma glycoprotein composed of paired sets of three subunits (α, β, γ). The protein plays a crucial role in protecting the vascular network against the loss of blood after tissue injury. The beta
Ralf Küppers et al.
Methods in molecular biology (Clifton, N.J.), 971, 49-63 (2013-01-09)
Normal and malignant B cells carry rearranged immunoglobulin (Ig) variable region genes, which due to their practically limitless diversity represent ideal clonal markers for these cells. We describe here an approach to isolate single cells from frozen tissue sections by
Scott S Maxwell et al.
RNA biology, 10(11), 1741-1757 (2013-11-26)
The discovery that Rett syndrome (RTT) is caused by mutation of the methyl-CpG-binding-protein MeCP2 provided a major breakthrough in understanding the neurodevelopmental disorder and accelerated MeCP2 research. However, gene regulation by MeCP2 is complicated. The current consensus for MeCP2 remains
Three-dimensional porous network structure developed in hydroxyapatite-based nanocomposites containing enzyme pretreated silk fibroin.
Wang Li, et al.
Journal of Nanoparticle Research, 6, 91-98 (2004)
Karen I Lange et al.
Human molecular genetics, 31(10), 1574-1587 (2021-12-30)
Better methods are required to interpret the pathogenicity of disease-associated variants of uncertain significance (VUS), which cannot be actioned clinically. In this study, we explore the use of an animal model (Caenorhabditis elegans) for in vivo interpretation of missense VUS
Gerhild Zauner et al.
Methods in molecular biology (Clifton, N.J.), 951, 113-127 (2013-01-09)
Liquid chromatography-tandem stage mass spectrometry of glycopeptides is a powerful tool for the site-specific glycosylation analysis of glycoproteins. Using fetuin as a model substance, we describe a protocol for glycopeptide dissection using nonspecific proteolysis by proteinase K. Proteolysis is achieved
Chromatin isolation by RNA purification (ChIRP).
Chu, C., et al.
Journal of Visualized Experiments (2012)
Todd W Osmundson et al.
Molecular ecology resources, 13(1), 66-74 (2012-11-06)
The ubiquity, high diversity and often-cryptic manifestations of fungi and oomycetes frequently necessitate molecular tools for detecting and identifying them in the environment. In applications including DNA barcoding, pathogen detection from plant samples, and genotyping for population genetics and epidemiology
Xinping Lin et al.
Yeast (Chichester, England), 29(11), 467-474 (2012-10-16)
Red yeasts hold great promise in the production of microbial lipids and carotenoids. Genetic study of red yeasts has attracted much attention; however, rapid amplification of genes from red yeast samples remains technically challenging. Here a highly efficient method for
Loïc Lionnard et al.
Cell death and differentiation, 26(5), 902-917 (2018-07-26)
BCL2A1 is an anti-apoptotic member of the BCL-2 family that contributes to chemoresistance in a subset of tumors. BCL2A1 has a short half-life due to its constitutive processing by the ubiquitin-proteasome system. This constitutes a major tumor-suppressor mechanism regulating BCL2A1
Amino acid sequence of proteinase K from the mold Tritirachium album Limber: Proteinase K ? a subtilisin-related enzyme with disulfide bonds.
Jany KD, et al.
Febs Letters, 199(2), 139-144 (1986)
Sudhir Kumar Shukla et al.
The Journal of antibiotics, 66(2), 55-60 (2012-11-15)
The dominant role of biofilm-associated protein (Bap) in Staphylococcus aureus biofilm development prompted us to investigate Bap as a potential target for proteinase-mediated biofilm dispersion. Biofilm assay in microtitre plates showed that proteinase K hampered the early adhesion of cells
Loïc Lionnard et al.
Cell death and differentiation, 26(5), 902-917 (2018-07-26)
BCL2A1 is an anti-apoptotic member of the BCL-2 family that contributes to chemoresistance in a subset of tumors. BCL2A1 has a short half-life due to its constitutive processing by the ubiquitin-proteasome system. This constitutes a major tumor-suppressor mechanism regulating BCL2A1
Tobias C Kunz et al.
Frontiers in cellular and infection microbiology, 9, 276-276 (2019-08-27)
Expansion microscopy (ExM) is a novel tool to improve the resolution of fluorescence-based microscopy that has not yet been used to visualize intracellular pathogens. Here we show the expansion of the intracellular pathogen Chlamydia trachomatis, enabling to differentiate its two
Eugene V Gasanov et al.
PloS one, 10(3), e0119711-e0119711 (2015-03-10)
BDNF and its specialized receptor TrkB are expressed in the developing lateral line system of zebrafish, but their role in this organ is unknown. To tackle this problem in vivo, we used transgenic animals expressing fluorescent markers in different cell
Margherita Y Turco et al.
Nature, 564(7735), 263-267 (2018-11-30)
The placenta is the extraembryonic organ that supports the fetus during intrauterine life. Although placental dysfunction results in major disorders of pregnancy with immediate and lifelong consequences for the mother and child, our knowledge of the human placenta is limited
Chia-Hsin Hsieh et al.
Neoplasia (New York, N.Y.), 20(8), 775-788 (2018-07-08)
Epithelial-mesenchymal transition (EMT) is a major event during cancer progression and metastasis; however, the definitive role of EMT in remodeling tumor microenvironments (TMEs) is unclear. Tumor-associated macrophages (TAMs) are a major type of host immune cells in TMEs, and they
Tobias C Kunz et al.
Frontiers in cellular and infection microbiology, 11, 644750-644750 (2021-04-03)
Expansion Microscopy (ExM) is a novel tool improving the resolution of fluorescence microscopy by linking the sample into a hydrogel that gets physically expanded in water. Previously, we have used ExM to visualize the intracellular Gram-negative pathogens Chlamydia trachomatis, Simkania
Rebecca Z Fan et al.
Acta neuropathologica communications, 7(1), 184-184 (2019-11-21)
Targeting alpha-synuclein (α-syn) as a therapeutic strategy for Parkinson's disease (PD) has been intensively pursued largely due to its well-recognized pathogenic role. Since its discovery as the first familial link to PD over two decades ago, this protein has been
Bridget M Arman et al.
Scientific reports, 13(1), 5646-5646 (2023-04-07)
Spontaneous preterm birth is the leading cause of perinatal morbidity and mortality. Tocolytics are drugs used in cases of imminent preterm birth to inhibit uterine contractions. Nifedipine is a calcium channel blocking agent used to delay threatened spontaneous preterm birth
Adam P Sharples et al.
Biogerontology, 17(3), 603-617 (2015-09-10)
Sufficient quantity and quality of skeletal muscle is required to maintain lifespan and healthspan into older age. The concept of skeletal muscle programming/memory has been suggested to contribute to accelerated muscle decline in the elderly in association with early life
Lianfeng Wu et al.
Cell, 167(7), 1705-1718 (2016-12-17)
Metformin has utility in cancer prevention and treatment, though the mechanisms for these effects remain elusive. Through genetic screening in C. elegans, we uncover two metformin response elements: the nuclear pore complex (NPC) and acyl-CoA dehydrogenase family member-10 (ACAD10). We demonstrate that
Jessica Varela Villarreal et al.
Journal of microbiological methods, 94(3), 161-169 (2013-07-03)
Molecular techniques, such as polymerase chain reaction (PCR) and quantitative PCR (qPCR), are very sensitive, but may detect total DNA present in a sample, including extracellular DNA (eDNA) and DNA coming from live and dead cells. DNase I is an
Ellen Marie Straarup et al.
Nucleic acids research, 38(20), 7100-7111 (2010-07-10)
The potency and specificity of locked nucleic acid (LNA) antisense oligonucleotides was investigated as a function of length and affinity. The oligonucleotides were designed to target apolipoprotein B (apoB) and were investigated both in vitro and in vivo. The high
Chao Wang et al.
Journal of medicinal chemistry, 56(6), 2527-2539 (2013-03-06)
The small molecule fusion inhibitors N-(4-carboxy-3-hydroxyphenyl)-2,5-dimethylpyrrole (NB-2) and N-(3-carboxy-4-hydroxyphenyl)-2,5-dimethylpyrrole (A12) target a hydrophobic pocket of HIV-1 gp41 and have moderate anti-HIV-1 activity. In this paper, we report the design, synthesis, and structure-activity relationship of a group of hybrid molecules in
Nadezda A Fursova et al.
Molecular cell, 74(5), 1020-1036 (2019-04-29)
The Polycomb system modifies chromatin and plays an essential role in repressing gene expression to control normal mammalian development. However, the components and mechanisms that define how Polycomb protein complexes achieve this remain enigmatic. Here, we use combinatorial genetic perturbation
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