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Showing 1-30 of 148 results for "PHG0006" within Papers
Nikhil Baban Ghate et al.
PloS one, 10(5), e0128221-e0128221 (2015-05-27)
Free iron typically leads to the formation of excess free radicals, and additional iron deposition in the liver contributes to the oxidative pathologic processes of liver disease. Many pharmacological properties of the insectivorous plant Drosera burmannii Vahl. have been reported
Allan Joaquim Lamontanara et al.
Nature communications, 5, 5470-5470 (2014-11-18)
The activity of protein kinases is regulated by multiple molecular mechanisms, and their disruption is a common driver of oncogenesis. A central and almost universal control element of protein kinase activity is the activation loop that utilizes both conformation and
James A Woo et al.
Journal of chromatography. A, 1407, 58-68 (2015-07-15)
A homologous ligand library based on the commercially-available Nuvia cPrime ligand was generated to systematically explore various features of a multimodal cation-exchange ligand and to identify structural variants that had significantly altered chromatographic selectivity. Substitution of the polar amide bond
Masahiro Omori et al.
Stem cell research & therapy, 6, 124-124 (2015-06-20)
Surface modification of titanium (Ti) implants promotes bone formation and shortens the osseointegration period. The aim of this study was to promote bone regeneration and stability around implants using atmospheric pressure plasma (APP) pretreatment. This was followed by immobilization of
Timothy J Brazil et al.
Veterinary journal (London, England : 1997), 202(3), 536-542 (2014-09-23)
The aim of this study was to characterise constitutive apoptosis in equine peripheral blood neutrophils, including assessment of factors that potentially modulate neutrophil survival through alteration of the rate of constitutive apoptosis. Cells underwent spontaneous time-dependent constitutive apoptosis when aged
Sujit Sadashiv Jagtap et al.
Applied and environmental microbiology, 80(14), 4207-4214 (2014-05-06)
Marine microbes use alginate lyases to degrade and catabolize alginate, a major cell wall matrix polysaccharide of brown seaweeds. Microbes frequently contain multiple, apparently redundant alginate lyases, raising the question of whether these enzymes have complementary functions. We report here
Sietse T Braakman et al.
Experimental eye research, 130, 87-96 (2014-12-03)
All aqueous humor draining through the conventional outflow pathway must cross the endothelium of Schlemm's canal (SC), likely by passing through micron-sized transendothelial pores. SC pores are non-uniformly distributed along the inner wall endothelium, but it is unclear how the
Zhongxin Zhou et al.
Biotechnology and applied biochemistry, 62(2), 268-274 (2014-06-04)
More information regarding the bactericidal properties of polyhexamethylene guanidine hydrochloride (PHMG) against clinically important antibiotic-resistant ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species) pathogens needs to be provided for its uses in infection control.
Juan P Bustamante et al.
Biochimica et biophysica acta, 1840(7), 2281-2288 (2014-04-08)
Understanding the molecular mechanism through which proteins are functional at extreme high and low temperatures is one of the key issues in structural biology. To investigate this phenomenon, we have focused on two instructive truncated hemoglobins from Thermobifida fusca (Tf-trHbO)
D Shortle
Journal of cellular biochemistry, 30(4), 281-289 (1986-01-01)
Several mutant forms of staphylococcal nuclease with one or two defined amino acid substitutions have been purified, and the effects of the altered amino acid sequence on the stability of the folded conformation have been analyzed by guanidine hydrochloride denaturation.
Luitgard M Graul-Neumann et al.
European journal of human genetics : EJHG, 22(6), 726-733 (2013-10-17)
Acromesomelic chondrodysplasias (ACDs) are characterized by disproportionate shortening of the appendicular skeleton, predominantly affecting the middle (forearms and forelegs) and distal segments (hands and feet). Here, we present two consanguineous families with missense (c.157T>C, p.(C53R)) or nonsense (c.657G>A, p.(W219*)) mutations
Nazish Ahmed et al.
Tissue engineering. Part A, 20(15-16), 2224-2233 (2014-03-13)
One of the factors preventing clinical application of regenerative medicine to degenerative cartilage diseases is a suitable source of cells. Chondrocytes, the only cell type of cartilage, grown in vitro under culture conditions to expand cell numbers lose their phenotype
C Derek Ma et al.
Nature, 517(7534), 347-350 (2015-01-17)
The structure of water near non-polar molecular fragments or surfaces mediates the hydrophobic interactions that underlie a broad range of interfacial, colloidal and biophysical phenomena. Substantial progress over the past decade has improved our understanding of hydrophobic interactions in simple
Saeed Emadi et al.
Biochemical and biophysical research communications, 450(4), 1339-1344 (2014-07-09)
Protein aggregation and its subsequent deposition in different tissues culminate in a diverse range of diseases collectively known as amyloidoses. Aggregation of hen or human lysozyme depends on certain conditions, namely acidic pH or the presence of additives. In the
Lavanya K Iyer et al.
Molecular pharmaceutics, 12(9), 3237-3249 (2015-07-24)
Protein structure and local environment in lyophilized formulations were probed using high-resolution solid-state photolytic cross-linking with mass spectrometric analysis (ssPC-MS). In order to characterize structure and microenvironment, protein-protein, protein-excipient, and protein-water interactions in lyophilized powders were identified. Myoglobin (Mb) was
Paul Human et al.
Journal of pharmaceutical sciences, 104(2), 722-730 (2014-12-03)
Pump delivery of human interferon alpha-2B (IFNα2b) has the potential for inducing immunogenic drug aggregates. We therefore evaluated the immunogenicity of mechanically induced IFNα2b aggregates to assess this risk. Transgenic human-IFNα2b (TG) and wild-type (WT) FVB/N mice (n = 8
Keitaro Hirawatari et al.
Mammalian genome : official journal of the International Mammalian Genome Society, 26(5-6), 248-256 (2015-05-13)
Infertility in humans and subfertility in domestic animals are two major reproductive problems. Among human couples, ~15% are diagnosed as infertile, and males are considered responsible in about 50% of the cases. To examine male fertility, various sperm tests including
David R Cooper et al.
Biophysical journal, 109(1), 66-75 (2015-07-15)
The N-methyl-D-aspartate receptor (NMDAR) is a member of the glutamate receptor family of proteins and is responsible for excitatory transmission. Activation of the receptor is thought to be controlled by conformational changes in the ligand binding domain (LBD); however, glutamate
Qi Chen et al.
Analytical and bioanalytical chemistry, 407(3), 1027-1032 (2014-10-20)
To analyze the proteome of an extremely low number of cells or even a single cell, we established a new method of digesting whole cells into mass-spectrometry-identifiable peptides in a single step within 2 h. Our sampling method greatly simplified the
Weronika E Borek et al.
Nature communications, 6, 7929-7929 (2015-08-06)
Microtubule nucleation is highly regulated during the eukaryotic cell cycle, but the underlying molecular mechanisms are largely unknown. During mitosis in fission yeast Schizosaccharomyces pombe, cytoplasmic microtubule nucleation ceases simultaneously with intranuclear mitotic spindle assembly. Cytoplasmic nucleation depends on the
Shin Koike et al.
Biochemical and biophysical research communications, 467(2), 361-366 (2015-10-04)
Although it's well known that protein carbonyl (PCO) and advanced glycation end-products (AGEs) levels are elevated in plasma from patients with renal dysfunction, we recently identified patients who had no renal dysfunction but possessed high levels of plasma pentosidine (PEN)
Amanda Phaelante et al.
Journal of cardiovascular translational research, 8(5), 328-337 (2015-06-19)
The antioxidant N-acetycysteine can turn into a prooxidant molecule in presence of iron ions. Thus, our goal was to test if the association of N-acetylcysteine (NAC) and an iron chelator (deferoxamine--DFX) in a rodent model of acute myocardial infarction (AMI)
Florian Platten et al.
The journal of physical chemistry. B, 119(48), 14986-14993 (2015-11-07)
Protein phase behavior and protein-protein interactions can be tuned by additives. We experimentally determined the phase behavior of lysozyme solutions, namely, the cloud-point temperature (CPT), in the presence of two additives, sodium chloride (NaCl) and guanidine hydrochloride (GuHCl). Their concentrations
K V Greco et al.
Journal of biomaterials applications, 30(2), 239-253 (2015-04-10)
Off-the-shelf availability of tissue-engineered skin constructs, tailored by different combinations of reagents to produce a highly preserved biological matrix is often the only means to help patients suffering skin damage. This study assessed the effect of five different decellularisation methods
Elamaran Meibalan et al.
Eukaryotic cell, 14(4), 371-384 (2015-02-11)
Malaria parasites replicating inside red blood cells (RBCs) export a large subset of proteins into the erythrocyte cytoplasm to facilitate parasite growth and survival. PTEX, the parasite-encoded translocon, mediates protein transport across the parasitophorous vacuolar membrane (PVM) in Plasmodium falciparum-infected
Angireddy Rajesh et al.
Journal of reproductive immunology, 110, 117-129 (2015-04-23)
Evaluating the immunocontraceptive potential of sperm-bound proteins is an active area of investigation. In this study, we analyzed the role of prostate- and testes-expressed (PATE) and PATE-F proteins in sperm function. Capacitation was measured as a function of tyrosine phosphorylation
Yuetian Yan et al.
Biochemistry, 54(34), 5322-5328 (2015-08-13)
The type 2 L-serine dehydratase from Legionella pneumophila (lpLSD) contains a [4Fe-4S](2+) cluster that acts as a Lewis acid to extract the hydroxyl group of L-serine during the dehydration reaction. Surprisingly, the crystal structure shows that all four of the
Ming Lei et al.
Journal of pharmaceutical sciences, 104(3), 995-1004 (2014-12-30)
The biopharmaceutical industry increasingly demands thorough characterization of protein conformation and conformational dynamics to ensure product quality and consistency. Here, we present a chromatography-based method that is able to characterize protein conformation and conformational dynamics at peptide level resolution in
Avisek Mahapa et al.
PloS one, 10(3), e0122168-e0122168 (2015-03-31)
SarA, a Staphylococcus aureus-specific dimeric protein, modulates the expression of numerous proteins including various virulence factors. Interestingly, S. aureus synthesizes multiple SarA paralogs seemingly for optimizing the expression of its virulence factors. To understand the domain structure/flexibility and the folding/unfolding
Ewa M Koźma et al.
Experimental biology and medicine (Maywood, N.J.), 239(12), 1575-1588 (2014-06-11)
Tumor transformation and progression both lead to extracellular matrix remodeling, which is also reflected in an alteration in the proportion of dermatan sulphate (DS) and chondroitin sulphate (CS) and an accumulation of the latter. In addition, a significant increase in
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