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Showing 1-16 of 16 results for "S6782" within Papers
Ya-Jie Zou et al.
Journal of microbiology (Seoul, Korea), 50(1), 72-78 (2012-03-01)
A novel laccase from the edible mushroom Hericium coralloides was purified by ion exchange chromatography on diethylaminoethyl (DEAE) cellulose, carboxymethyl (CM) cellulose, and Q-Sepharose columns followed by fast protein liquid chromatography gel filtration on a Superdex 75 column. Analysis by
J Oh et al.
Human molecular genetics, 9(3), 375-385 (2000-02-03)
Hermansky-Pudlak syndrome (HPS) is a rare autosomal recessive disorder in which oculocutaneous albinism, bleeding tendency and a ceroid-lipofuscin lysosomal storage disease result from defects of multiple cytoplasmic organelles: melanosomes, platelet dense granules and lysosomes. The HPS polypeptide, a 700 amino
W Noppe et al.
Journal of dairy science, 87(10), 3247-3255 (2004-09-21)
Linear pentadecamer and cyclic hexamer peptide phage libraries were used to isolate phage clones with binding affinity toward lactoferrins purified from human and bovine milk. Phage clones with high specificity toward lactoferrin were selected with different binding strengths depending on
G Iberer et al.
Journal of chromatography. A, 921(1), 15-24 (2001-07-20)
In size-exclusion chromatography (SEC), proteins and peptides are separated according to their molecular size in solution. SEC is especially useful as an effective fractionation step to separate a vast amount of impurities from the components of interest and/or as final
Weidong Zhu et al.
Biochemistry, 47(23), 6226-6232 (2008-05-15)
Human cystathionine-gamma-lyase (CGL) is a pyridoxal-5'-phosphate (PLP)-dependent enzyme, which functions in the transsulfuration pathway that converts homocysteine to cysteine. In addition, CGL is one of two major enzymes that can catalyze the formation of hydrogen sulfide, an important gaseous signaling
Thomas Ouellette et al.
Protein expression and purification, 30(2), 156-166 (2003-07-26)
A recombinant form of human rhIL-7 was overexpressed in Escherichia coli HMS174 (DE3) pLysS under the control of a T7 promoter. The resulting insoluble inclusion bodies were separated from cellular debris by cross-flow filtration and solubilized by homogenization with 6
J L Van Hove et al.
Biochemistry and molecular biology international, 43(3), 613-623 (1997-11-14)
Large quantities of recombinant acid alpha-glucosidase are needed for in vivo experimentation of enzyme replacement therapy in Pompe disease. We describe a new purification method for the purification of this recombinant enzyme from tissue culture medium consisting of concanavalin A
Mario Jiz et al.
Infection and immunity, 76(7), 3164-3169 (2008-04-23)
Despite effective chemotherapy, schistosomiasis remains a major public health problem in the developing world, with at least 200 million active infections resulting in significant morbidity. Rapid reinfection after treatment, accompanied by extensive residual morbidity, mandates alternative control strategies, including vaccine
Evandro Fei Fang et al.
Archives of toxicology, 86(2), 293-304 (2011-10-05)
A 20-kDa Kunitz-type trypsin-chymotrypsin inhibitor, Bauhinia purpurea trypsin inhibitor (BPLTI), has been isolated from the seeds of B. purpurea L. by using liquid chromatography procedures that involved ion exchange chromatography on Sp-Sepharose and Mono S and gel filtration on Superdex
J Wijkander et al.
Endocrinology, 139(1), 219-227 (1998-01-08)
Insulin stimulation of adipocytes results in serine phosphorylation/activation of phosphodiesterase 3B (PDE 3B) and activation of a kinase that phosphorylates PDE 3B in vitro, key events in the antilipolytic action of this hormone. We have investigated the role for p70
Anna Lundquist et al.
Biomedical chromatography : BMC, 20(1), 83-87 (2005-06-15)
We studied the effect of charged lipids or detergent on the retention of drugs and an oligonucleotide by immobilized liposome chromatography to characterize solute-membrane interactions. This is a novel approach in analysis of oligonucleotide-liposome interactions. The charged lipids (phosphatidylserine or
Purification of sucrose synthase from rice and its protein-chemical characterization.
Elling, L., and Kula, M.-R.
Journal of Biotechnology, 29(3), 277-286 (1993)
Marcel Zamocky et al.
The Biochemical journal, 418(2), 443-451 (2008-11-13)
Phytopathogenic fungi such as the rice blast fungus Magnaporthe grisea are unique in having two catalase/peroxidase (KatG) paralogues located either intracellularly (KatG1) or extracellularly (KatG2). The coding genes have recently been shown to derive from a lateral gene transfer from
T Sato et al.
Journal of the American Society of Nephrology : JASN, 10(6), 1242-1252 (1999-06-11)
Mesangial cell (MC) injury is a characteristic feature in the early phase of Thy.1 nephritis. The present study investigates the contribution of complement to MC apoptosis in this experimental model of kidney disease in rats. Thy.1 nephritis was induced by
A Uretschläger et al.
Journal of chromatography. A, 908(1-2), 243-250 (2001-02-24)
The concept of annular chromatography was tested by separation of a real protein solution used in biotechnology. Green fluorescent protein was expressed in S. cerevisiae and the extract was continuously separated by a pressurized annular chromatograph packed with a Superdex
Partial purification of two proteins (100 kDa and 67 kDa) cooperating in the ATP-dependent inactivation of spinach leaf nitrate reductase.
Spill, D., and Kaiser, W.M.
Planta, 192(2), 183-188 (1994)
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