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Showing 1-30 of 30 results for "S8398" within Papers
Maria-Del-Carmen Diaz-de-la-Loza et al.
Development (Cambridge, England), 147(5) (2020-03-04)
Mutations in the Ultrabithorax (Ubx) gene cause homeotic transformation of the normally two-winged Drosophila into a four-winged mutant fly. Ubx encodes a HOX family transcription factor that specifies segment identity, including transformation of the second set of wings into rudimentary
Karin Tanabe et al.
Bio-protocol, 7(8), e2225-e2225 (2017-04-20)
In this protocol, we provide an experimental procedure that perform time-lapse observation of intra-cellular structures such as chromosomes, cytoskeletons and cell organelles during meiotic cell divisions in Drosophila males. As primary spermatocyte is the largest dividing diploid cell in Drosophila
Banseok Lee et al.
Biochemical and biophysical research communications, 550, 184-190 (2021-03-12)
Linear ubiquitination is an atypic ubiquitination process that directly connects the N- and C-termini of ubiquitin and is catalyzed by HOIL-1-interacting protein (HOIP). It is involved in the immune response or apoptosis by activating the nuclear factor-κB pathway and is
Yuankai Dong et al.
Genetics, 215(1), 89-101 (2020-03-18)
BEAF (Boundary Element-Associated Factor) was originally identified as a Drosophila melanogaster chromatin domain insulator-binding protein, suggesting a role in gene regulation through chromatin organization and dynamics. Genome-wide mapping found that BEAF usually binds near transcription start sites, often of housekeeping
Mukesh Maharjan et al.
Genetics, 215(4), 1003-1012 (2020-06-20)
Chromatin domain insulators are thought to help partition the genome into genetic units called topologically associating domains (TADs). In Drosophila, TADs are often separated by inter-TAD regions containing active housekeeping genes and associated insulator binding proteins. This raises the question
Silke Rothenbusch-Fender et al.
Biology open, 6(12), 1876-1888 (2017-11-11)
Alind Gupta et al.
Journal of cell science, 131(16) (2018-07-29)
Cilia are cellular antennae that are essential for human development and physiology. A large number of genetic disorders linked to cilium dysfunction are associated with proteins that localize to the ciliary transition zone (TZ), a structure at the base of
Yoon Seok Suh et al.
Molecules and cells, 43(3), 304-311 (2020-01-17)
Methionyl-tRNA synthetase (MRS) is essential for translation. MRS mutants reduce global translation, which usually increases lifespan in various genetic models. However, we found that MRS inhibited Drosophila reduced lifespan despite of the reduced protein synthesis. Microarray analysis with MRS inhibited
Fabien Cannac et al.
Science advances, 6(16), eaay7928-eaay7928 (2020-06-05)
The Hedgehog (Hh) signaling pathway controls embryonic development and adult tissue homeostasis in multicellular organisms. In Drosophila melanogaster, the pathway is primed by secretion of a dually lipid-modified morphogen, Hh, a process dependent on a membrane-integral protein Dispatched. Although Dispatched
Sarah Mische et al.
Molecular biology of the cell, 19(11), 4918-4929 (2008-09-19)
The dynein light intermediate chain (LIC) is a subunit unique to the cytoplasmic form of dynein, but how it contributes to dynein function is not fully understood. Previous work has established that the LIC homodimer binds directly to the dynein
Amy Platenkamp et al.
Molecular biology of the cell, 31(21), 2379-2397 (2020-08-21)
To identify novel regulators of nonmuscle myosin II (NMII) we performed an image-based RNA interference screen using stable Drosophila melanogaster S2 cells expressing the enhanced green fluorescent protein (EGFP)-tagged regulatory light chain (RLC) of NMII and mCherry-Actin. We identified the
Jeong-Hoon Han et al.
Cell death & disease, 11(11), 944-944 (2020-11-04)
Wg/Wnt as a signaling protein binds to Frizzled (Fz) and Arrow (Arr), two Wg co-receptors essential for Wg signaling for cell proliferation, differentiation, and cell survival. Arr has a long extracellular region, a single transmembrane domain and an intracellular region.
Constance Alabert et al.
Cell reports, 30(4), 1223-1234 (2020-01-30)
Chromatin states must be maintained during cell proliferation to uphold cellular identity and genome integrity. Inheritance of histone modifications is central in this process. However, the histone modification landscape is challenged by incorporation of new unmodified histones during each cell
Kathleen R Stewart-Morgan et al.
Molecular cell, 75(2), 284-297 (2019-05-28)
DNA replication is highly disruptive to chromatin, leading to eviction of nucleosomes, RNA polymerase, and regulatory factors. When and how transcription resumes on DNA following DNA replication is unknown. Here we develop a replication-coupled assay for transposase-accessible chromatin (repli-ATAC-seq) to
Banseok Lee et al.
Biochemical and biophysical research communications, 550, 184-190 (2021-03-12)
Linear ubiquitination is an atypic ubiquitination process that directly connects the N- and C-termini of ubiquitin and is catalyzed by HOIL-1-interacting protein (HOIP). It is involved in the immune response or apoptosis by activating the nuclear factor-κB pathway and is
Min Zhao et al.
Genetics, 178(4), 1947-1971 (2008-04-24)
Fasciclin2 (Fas2) and Discslarge (Dlg) localize to the basolateral junction (BLJ) of Drosophila follicle epithelial cells and inhibit their proliferation and invasion. To identify a BLJ signaling pathway we completed a genomewide screen for mutants that enhance dlg tumorigenesis. We
Feiyue Lu et al.
Molecular cell, 73(6), 1232-1242 (2019-02-16)
The C-terminal domain (CTD) of RNA polymerase II (Pol II) is composed of repeats of the consensus YSPTSPS and is an essential binding scaffold for transcription-associated factors. Metazoan CTDs have well-conserved lengths and sequence compositions arising from the evolution of
Jonathan J Ipsaro et al.
Cell reports, 34(13), 108914-108914 (2021-04-01)
The Piwi-interacting RNA (piRNA) pathway safeguards genomic integrity by silencing transposable elements (transposons) in the germline. While Piwi is the central piRNA factor, others including Asterix/Gtsf1 have also been demonstrated to be critical for effective silencing. Here, using enhanced crosslinking
Dong-Gyu Cho et al.
Molecules and cells, 44(1), 13-25 (2021-01-30)
Apoptosis and compensatory proliferation, two intertwined cellular processes essential for both development and adult homeostasis, are often initiated by the mis-regulation of centrosomal proteins, damaged DNA, and defects in mitosis. Fly Anastral spindle 3 (Ana3) is a member of the
Crissie Vandehoef et al.
Cell reports, 31(10), 107736-107736 (2020-06-11)
Dietary nutrients shape complex interactions between hosts and their commensal gut bacteria, further promoting flexibility in host-microbiota associations that can drive nutritional symbiosis. However, it remains less clear if diet-dependent host signaling mechanisms also influence these associations. Using Drosophila, we
Zsolt Lakatos et al.
International journal of molecular sciences, 22(19) (2021-10-14)
Lysosomal degradation, the common destination of autophagy and endocytosis, is one of the most important elements of eukaryotic metabolism. The small GTPases Rab39A and B are potential new effectors of this pathway, as their malfunction is implicated in severe human
Kentaro Kato et al.
PLoS biology, 9(8), e1001133-e1001133 (2011-09-14)
Organisms are structurally robust, as cells accommodate changes preserving structural integrity and function. The molecular mechanisms underlying structural robustness and plasticity are poorly understood, but can be investigated by probing how cells respond to injury. Injury to the CNS induces
Ahmed Sabri et al.
PloS one, 6(4), e18847-e18847 (2011-04-29)
Aphidius ervi (Hymenoptera: Braconidae) is an entomophagous parasitoid known to be an effective parasitoid of several aphid species of economic importance. A reduction of its production cost during mass rearing for inundative release is needed to improve its use in
Louis Philip Benoit Bouvrette et al.
RNA (New York, N.Y.), 24(1), 98-113 (2017-10-29)
Cells are highly asymmetrical, a feature that relies on the sorting of molecular constituents, including proteins, lipids, and nucleic acids, to distinct subcellular locales. The localization of RNA molecules is an important layer of gene regulation required to modulate localized
Tim Hundertmark et al.
PloS one, 13(9), e0203622-e0203622 (2018-09-08)
Spermatogenesis in many species including Drosophila melanogaster is accompanied by major reorganisation of chromatin in post-meiotic stages, involving a nearly genome-wide displacement of histones by protamines, Mst77F and Protamine-like 99C. A proposed prerequisite for the histone-to-protamine transition is massive histone
Benjamin P Towler et al.
PLoS genetics, 16(12), e1009297-e1009297 (2020-12-29)
Dis3L2 is a highly conserved 3'-5' exoribonuclease which is mutated in the human overgrowth disorders Perlman syndrome and Wilms' tumour of the kidney. Using Drosophila melanogaster as a model system, we have generated a new dis3L2 null mutant together with
Stefanie M K Gärtner et al.
European journal of cell biology, 94(1), 46-59 (2014-12-04)
Spermatogenesis is accompanied by a remarkable reorganization of the chromatin in post-meiotic stages, characterized by a near genome-wide displacement of histones by protamines and a transient expression of transition proteins. In Drosophila, the transition-protein-like protein Tpl94D contains an HMG-box domain
Y Asano et al.
HFSP journal, 3(3), 194-203 (2009-07-30)
Lamellipodia are broad actin-based structures that define the protruding edge of many motile animal cells. Here we identify a Drosophila homolog of the p21-activated kinases (Paks) as a novel inhibitor of Rac-mediated lamellipodial formation: Pak3 overexpression mimics a loss of
Gerald Stampfel et al.
Nature, 528(7580), 147-151 (2015-11-10)
One of the most important questions in biology is how transcription factors (TFs) and cofactors control enhancer function and thus gene expression. Enhancer activation usually requires combinations of several TFs, indicating that TFs function synergistically and combinatorially. However, while TF
Gábor Csordás et al.
eLife, 9 (2020-10-08)
Blood development in multicellular organisms relies on specific tissue microenvironments that nurture hematopoietic precursors and promote their self-renewal, proliferation, and differentiation. The mechanisms driving blood cell homing and their interactions with hematopoietic microenvironments remain poorly understood. Here, we use the
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