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Showing 1-30 of 7519 results for "SHRNA" within Papers
Daisuke Fujisawa et al.
The Journal of allergy and clinical immunology, 134(3), 622-633 (2014-06-24)
Wheal reactions to intradermally injected neuropeptides, such as substance P (SP) and vasoactive intestinal peptide, are significantly larger and longer lasting in patients with chronic urticaria (CU) than in nonatopic control (NC) subjects. Mas-related gene X2 (MrgX2) has been identified
Panagiotis Maragozidis et al.
Molecular cancer, 14, 187-187 (2015-11-07)
Lung cancer is the leading cause of cancer mortality worldwide, mainly due to late diagnosis, poor prognosis and tumor heterogeneity. Thus, the need for biomarkers that will aid classification, treatment and monitoring remains intense and challenging and depends on the
Xiaofan Han et al.
Nature communications, 8, 14639-14639 (2017-03-04)
Endothelial exocytosis of Weibel-Palade body (WPB) is one of the first lines of defence against vascular injury. However, the mechanisms that control WPB exocytosis in the final stages (including the docking, priming and fusion of granules) are poorly understood. Here
Y Wang et al.
Oncogene, 35(11), 1363-1372 (2015-08-04)
RNF126 is an E3 ubiquitin ligase. The deletion of RNF126 gene was observed in a wide range of human cancers and is correlated with improved disease-free and overall survival. These data highlight the clinical relevance of RNF126 in tumorigenesis and
Antonis C Antoniou et al.
The New England journal of medicine, 371(6), 497-506 (2014-08-08)
Germline loss-of-function mutations in PALB2 are known to confer a predisposition to breast cancer. However, the lifetime risk of breast cancer that is conferred by such mutations remains unknown. We analyzed the risk of breast cancer among 362 members of
Carole Siret et al.
British journal of cancer, 113(10), 1445-1453 (2015-10-30)
Malignant transformation of melanocytes frequently coincides with an alteration in the expression of cell-cell adhesion molecules (cadherins) and cell-extracellular matrix proteins (integrins). How these two adhesion systems interplay to impact on cell invasion remains to be described in melanoma. Cell
HĆ©lĆØne Lazareth et al.
Nature communications, 10(1), 3303-3303 (2019-07-26)
The mechanisms driving the development of extracapillary lesions in focal segmental glomerulosclerosis (FSGS) and crescentic glomerulonephritis (CGN) remain poorly understood. A key question is how parietal epithelial cells (PECs) invade glomerular capillaries, thereby promoting injury and kidney failure. Here we
Plasmids: shopping in the age of plenty.
Vivien Marx
Nature methods, 11(8), 795-798 (2014-07-31)
Maria Czarnek et al.
Molecular therapy. Nucleic acids, 26, 711-731 (2021-10-28)
In parallel with the expansion of RNA interference (RNAi) techniques, accumulating evidence indicates that RNAi analyses might be seriously biased due to the off-target effects of gene-specific short hairpin RNAs (shRNAs). Our findings indicated that off-target effects of non-targeting shRNA
Maria C Guimaro et al.
Human gene therapy, 31(19-20), 1068-1073 (2020-03-17)
Adeno-associated virus (AAV) vector technology is rapidly advancing and becoming not only the leading vector platform in the field of gene therapy but also a useful tool for functional genomic studies of novel proteins. As most vectors utilize constitutive promoters
Sharmin Seraj et al.
Biochemical pharmacology, 166, 192-202 (2019-05-28)
Duration of gene silencing due to the short-term silencing effects induced by exogenous siRNA have limited the therapeutic applications of RNAi and the development of RNAi-based therapeutics. We here generated Eg5 shRNA-expressing plasmids using the inverted terminal repeats (ITRs) sequences
Li-jun Mao et al.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 36(11), 9073-9081 (2015-06-19)
Recent studies suggest that SATB1 is a promising therapeutic target for prostate cancer. To develop novel SATB1-based therapeutic agents for prostate cancer, in this study, we aimed to construct ZD55-SATB1, an oncolytic adenovirus ZD55 carrying shRNA targeting SATB1, and investigate
Hui Qiao et al.
Technology in cancer research & treatment, 17, 1533033818784411-1533033818784411 (2018-07-11)
Ī±-Enolase is a significant subunit of enolase and acts as a glycolytic enzyme responsible for catalyzing the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the anaerobic glycolysis pathway. The research about their role is known little in tumor invasion and metastasis.
Li Zhang et al.
Pharmaceutics, 13(10) (2021-10-24)
Obesity is a chronic systemic inflammatory disease, which occurs when energy intake exceeds the energy consumption. Therefore, controlling energy intake or increasing physical consumption can effectively control obesity. However, in reality, it is very difficult for the majority of obese
Lentivirus-mediated shRNA Targeting CNN2 Inhibits Hepatocarcinoma
Xueqing Kang et al.
International journal of medical sciences, 15(1), 69-76 (2018-01-16)
Christopher A de Solis et al.
Neurobiology of learning and memory, 124, 34-47 (2015-07-18)
The use of viral vector technology to deliver short hairpin RNAs (shRNAs) to cells of the nervous system of many model organisms has been widely utilized by neuroscientists to study the influence of genes on behavior. However, there have been
Wen Xiao et al.
Molecular pharmaceutics, 17(1), 229-238 (2019-11-26)
Eukaryotic translation initiation factors 3i (eIF3i) is a proto-oncogene that is overexpressed in various tumors, reducing its expression by eIF3i shRNA is a promising strategy to inhibit tumor growth or metastasis. Tumor cell is the target of eIF3i shRNA so
Sujin Kang et al.
Cellular signalling, 27(6), 1214-1224 (2015-03-10)
We previously showed that an increase of cellular Bcl-xL mediates acquired resistance to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and knockdown of Bcl-xL expression greatly sensitized TRAIL-induced cytotoxicity. Here, we show that Daxx downregulation increases the anti-tumorigenic activity through enhancement
Cristina Ana Mocanu et al.
Journal of controlled release : official journal of the Controlled Release Society, 338, 754-772 (2021-09-17)
The receptor for advanced glycation end products (RAGE) plays a central role in the chronic inflammatory process associated with atherosclerosis development. We aimed to develop lipoplexes carrying RAGE-short hairpin (sh) RNA, targeted to the adhesion molecule P-selectin, selectively expressed on
Zhenzhen Chen et al.
Small (Weinheim an der Bergstrasse, Germany), 11(8), 952-962 (2014-10-22)
Polycationic vectors have been used to deliver short hairpin RNAs (shRNAs) to knock-down genes for cancer therapies, but their inefficiency in lysosomal escape and shRNA release causes their low gene transcription efficiency. Herein, a three-layered polyethyleneimine (PEI)-coated gold nanocomplex interlaid
Jesse Stombaugh et al.
Journal of biomolecular screening, 20(8), 965-975 (2015-03-18)
RNA interference screening using pooled, short hairpin RNA (shRNA) is a powerful, high-throughput tool for determining the biological relevance of genes for a phenotype. Assessing an shRNA pooled screen's performance is difficult in practice; one can estimate the performance only
Hye Jin Kim et al.
Biomaterials, 177, 1-13 (2018-06-09)
Overexpression and knockdown of specific proteins can control stem cell differentiation for therapeutic purposes. In this study, we fabricated RUNX2, SOX9, and C/EBPĪ± plasmid DNAs (pDNAs) and ATF4-targeting shRNA (shATF4) to induce osteogenesis, chondrogenesis, and adipogenesis of human mesenchymal stem
Virginie Mournetas et al.
Journal of biological methods, 3(4), e55-e55 (2016-10-04)
Gene silencing techniques, including RNA interference methodologies, are widely used in reverse genetics to study the role of specific genes in biological processes. RNA interference has become easier to implement thanks to the RNAi Consortium (TRC), which has developed libraries
Sara Ayatollahi et al.
The international journal of biochemistry & cell biology, 92, 210-217 (2017-10-17)
RNAi-based gene therapy has been recently considered as a promising approach against cancer. Targeted delivery of drug, gene or therapeutic RNAi-based systems to tumor cells is one of the important issues in order to reduce side effects on normal cells.
Liam D Cassidy et al.
Autophagy, 14(7), 1256-1266 (2018-07-13)
Macroautophagy/autophagy is an evolutionarily conserved catabolic pathway whose modulation has been linked to diverse disease states, including age-associated disorders. Conventional and conditional whole-body knockout mouse models of key autophagy genes display perinatal death and lethal neurotoxicity, respectively, limiting their applications
Alessandro Bertero et al.
Current protocols in stem cell biology, 44, 5C-5C (2018-03-08)
The difficulties involved in conditionally perturbing complex gene expression networks represent major challenges toward defining the mechanisms controlling human development, physiology, and disease. We developed an OPTimized inducible KnockDown (OPTiKD) platform that addresses the limitations of previous approaches by allowing
Jose A Morales-Garcia et al.
Neurobiology of aging, 36(2), 1160-1173 (2014-12-03)
Different studies have suggested that the nucleotide cyclic adenosine 3', 5'-monophosphate can actively play an important role as a neuroprotective and anti-inflammatory agent after a brain injury. The phosphodiesterase 7 (PDE7) enzyme is one of the enzymes responsible for controlling
Jianfa Li et al.
Journal of experimental & clinical cancer research : CR, 35(1), 99-99 (2016-06-23)
Long non-coding RNAs (lncRNAs) have been proved to act as key molecules in cancer development and progression. Dysregulation of lncRNAs is discovered in various tumor tissues and cancer cells where they can serve as oncogenes or tumor suppressors. Long non-coding
Hiroki Kobayashi et al.
Biochemical and biophysical research communications, 467(1), 121-127 (2015-09-26)
Combining glycolytic inhibition with other anti-cancer therapies is a potential approach to treating cancer. In this context, we attempted to identify genes that determine sensitivity to 2-deoxyglucose (2DG), a glycolytic inhibitor, in cancer cells using pooled shRNA libraries targeting ā¼15,000
Sander B Frank et al.
BMC biotechnology, 17(1), 24-24 (2017-03-02)
Short hairpin RNA (shRNA) is an established and effective tool for stable knock down of gene expression. Lentiviral vectors can be used to deliver shRNAs, thereby providing the ability to infect most mammalian cell types with high efficiency, regardless of
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