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Showing 1-30 of 58 results for "T7408" within Papers
Remi Hatinguais et al.
Frontiers in immunology, 12, 641495-641495 (2021-04-13)
Reactive Oxygen Species (ROS) are highly reactive molecules that can induce oxidative stress. For instance, the oxidative burst of immune cells is well known for its ability to inhibit the growth of invading pathogens. However, ROS also mediate redox signalling
Carlos G Gonzalez et al.
mSystems, 5(3) (2020-07-02)
Stool-based proteomics is capable of significantly augmenting our understanding of host-gut microbe interactions. However, compared to competing technologies, such as metagenomics and 16S rRNA sequencing, it is underutilized due to its low throughput and the negative impact sample contaminants can
Alban Ordureau et al.
Molecular cell, 70(2), 211-227 (2018-04-17)
Flux through kinase and ubiquitin-driven signaling systems depends on the modification kinetics, stoichiometry, primary site specificity, and target abundance within the pathway, yet we rarely understand these parameters and their spatial organization within cells. Here we develop temporal digital snapshots
Bo Shen et al.
Cell, 182(1), 59-72 (2020-06-04)
Early detection and effective treatment of severe COVID-19 patients remain major challenges. Here, we performed proteomic and metabolomic profiling of sera from 46 COVID-19 and 53 control individuals. We then trained a machine learning model using proteomic and metabolomic measurements
Stefan Hinz et al.
iScience, 24(9), 103026-103026 (2021-09-16)
Age is the major risk factor in most carcinomas, yet little is known about how proteomes change with age in any human epithelium. We present comprehensive proteomes comprised of >9,000 total proteins and >15,000 phosphopeptides from normal primary human mammary
Jeremy S Toma et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 35(3), 1291-1306 (2015-01-23)
Induced pluripotent cell-derived motoneurons (iPSCMNs) are sought for use in cell replacement therapies and treatment strategies for motoneuron diseases such as amyotrophic lateral sclerosis (ALS). However, much remains unknown about the physiological properties of iPSCMNs and how they compare with
Vayu Maini Rekdal et al.
Science (New York, N.Y.), 364(6445) (2019-06-15)
The human gut microbiota metabolizes the Parkinson's disease medication Levodopa (l-dopa), potentially reducing drug availability and causing side effects. However, the organisms, genes, and enzymes responsible for this activity in patients and their susceptibility to inhibition by host-targeted drugs are
Haopeng Xiao et al.
Cell, 180(5), 968-983 (2020-02-29)
Mammalian tissues engage in specialized physiology that is regulated through reversible modification of protein cysteine residues by reactive oxygen species (ROS). ROS regulate a myriad of biological processes, but the protein targets of ROS modification that drive tissue-specific physiology in vivo
Monalisa T Manhanzva et al.
Scientific reports, 10(1), 6196-6196 (2020-04-12)
Female genital tract (FGT) inflammation increases HIV infection susceptibility. Non-optimal cervicovaginal microbiota, characterized by depletion of Lactobacillus species and increased bacterial diversity, is associated with increased FGT cytokine production. Lactobacillus species may protect against HIV partly by reducing FGT inflammation.
Identification of Rosmarinic Acid-Adducted Sites in Meat Proteins in a Gel Model under Oxidative Stress by Triple TOF MS/MS.
Tang CB, et al.
Journal of Agricultural and Food Chemistry, 64(33), 6466-6476 (2016)
Marco L Hennrich et al.
Analytical chemistry, 81(18), 7814-7822 (2009-08-20)
In proteomics, proteolytic peptides are often chemically modified to improve MS analysis, peptide identification, and/or to enable protein/peptide quantification. It is known that such chemical modifications can alter peptide fragmentation in collision induced dissociation MS/MS. Here, we investigated the fragmentation
Yuta Ohtani et al.
Journal of bioscience and bioengineering, 128(3), 379-383 (2019-04-09)
Proteome is extremely complex as many proteins with a large dynamic range are involved. Nano-liquid chromatography/mass spectrometry-based proteomics has made it possible to separate and identify thousands of proteins in one shot. Although the number of identified proteins in proteomics
Caterina Peggion et al.
Molecular neurobiology, 56(5), 3420-3436 (2018-08-22)
The cellular prion protein (PrPC), whose misfolded conformers are implicated in prion diseases, localizes to both the presynaptic membrane and postsynaptic density. To explore possible molecular contributions of PrPC to synaptic transmission, we utilized a mass spectrometry approach to quantify
Yi-Wen Chang et al.
Molecular & cellular proteomics : MCP, 19(11), 1805-1825 (2020-08-14)
The EGFR tyrosine kinase inhibitor gefitinib is commonly used for lung cancer patients. However, some patients eventually become resistant to gefitinib and develop progressive disease. Here, we indicate that ecto-ATP synthase, which ectopically translocated from mitochondrial inner membrane to plasma
Xiaofeng Guo et al.
Analytical biochemistry, 426(1), 86-90 (2012-04-12)
Bottom-up proteomics requires the digestion of proteins into peptides by processes that use salts for denaturing and buffering purposes. These salts need to be removed prior to mass spectrometry analysis to reduce ion suppression; solid-phase extraction (SPE) is a commonly
Anthony J Covarrubias et al.
Nature metabolism, 2(11), 1265-1283 (2020-11-18)
Declining tissue nicotinamide adenine dinucleotide (NAD) levels are linked to ageing and its associated diseases. However, the mechanism for this decline is unclear. Here, we show that pro-inflammatory M1-like macrophages, but not naive or M2 macrophages, accumulate in metabolic tissues
Amos Fumagalli et al.
Nature communications, 11(1), 4855-4855 (2020-09-27)
The atypical chemokine receptor 3 (ACKR3) plays a pivotal role in directing the migration of various cellular populations and its over-expression in tumors promotes cell proliferation and invasiveness. The intracellular signaling pathways transducing ACKR3-dependent effects remain poorly characterized, an issue
Jeremy A Herrera et al.
Clinical proteomics, 17, 24-24 (2020-06-23)
Haematoxylin and eosin (H&E)-which respectively stain nuclei blue and other cellular and stromal material pink-are routinely used for clinical diagnosis based on the identification of morphological features. A richer characterization can be achieved by laser capture microdissection coupled to mass
Chunmei Jin et al.
Journal of neurochemistry, 150(6), 776-786 (2019-06-20)
The SH3 and multiple ankyrin repeat domains 3 (Shank3) proteins are core organizers of the postsynaptic density in neuronal excitatory synapses, and their defects cause various neurodevelopmental and neuropsychiatric disorders. Mechanistically, Shank3 directly and indirectly interacts with hundreds of synaptic
Young Eun Kim et al.
STAR protocols, 2(4), 100849-100849 (2021-10-07)
Because glioblastoma (GBM) exhibits high heterogeneity, it is desirable to use patient-derived cells from the first stage of screening for GBM drug discovery. Here, we describe a protocol to culture patient-derived GBM cells on the extracellular matrix-coated plates to allow
Evaluation of the Effect of Trypsin Digestion Buffers on Artificial Deamidation
Hao P, et al.
Journal of Proteome Research, 14(2), 1308-1314 (2015)
C W Mahoney et al.
Analytical biochemistry, 138(1), 246-251 (1984-04-01)
The separation of 5'-adenosine di- and triphosphates from inorganic pyrophosphate or imidodiphosphate can be accomplished with reverse-phase HPLC by using a solvent system buffered by triethylammonium bicarbonate (pH 6.7). This buffer was used because it was neutral, readily volatile at
Sunil Kumar Yadav et al.
BMC microbiology, 20(1), 216-216 (2020-07-22)
A rice associated bacterium Burkholderia gladioli strain NGJ1 demonstrates mycophagy, a phenomenon wherein bacteria feed on fungi. Previously, we have reported that NGJ1 utilizes type III secretion system (T3SS) to deliver a prophage tail-like protein (Bg_9562) into fungal cells to
Suman Rao et al.
Cell chemical biology, 26(6), 818-829 (2019-04-16)
Covalent kinase inhibitors, which typically target cysteine residues, represent an important class of clinically relevant compounds. Approximately 215 kinases are known to have potentially targetable cysteines distributed across 18 spatially distinct locations proximal to the ATP-binding pocket. However, only 40
Yang Yu et al.
BMC genomics, 19(1), 742-742 (2018-10-12)
Gray leaf spot (GLS), which is caused by the necrotrophic fungi Cercospora zeae-maydis and Cercospora zeina, is one of the most impactful diseases in maize worldwide. The aim of the present study is to identify the resistance genes and understand
Yang Li et al.
ACS applied materials & interfaces, 8(42), 28437-28447 (2016-09-28)
The possibility that nanomaterials could perturb the normal course of an inflammatory response is a key issue when assessing nanoimmunosafety. The alteration of the normal progress of an inflammatory response may have pathological consequences, since inflammation is a major defensive
R Glen Uhrig et al.
The Plant journal : for cell and molecular biology, 99(1), 176-194 (2019-03-29)
Protein phosphorylation and acetylation are the two most abundant post-translational modifications (PTMs) that regulate protein functions in eukaryotes. In plants, these PTMs have been investigated individually; however, their co-occurrence and dynamics on proteins is currently unknown. Using Arabidopsis thaliana, we
Jingli Tao et al.
Autophagy, 14(11), 1850-1869 (2018-07-10)
To explore the anti-inflammatory activity of endogenous produced melatonin, a melatonin-enriched animal model (goat) with AANAT transfer was successfully generated with somatic cell nuclear transfer (SCNT) technology. Basically, a pIRES2-EGFP-AANAT expression vector was constructed and was transferred into the female
Noura Alzahofi et al.
Nature communications, 11(1), 3495-3495 (2020-07-15)
Cell biologists generally consider that microtubules and actin play complementary roles in long- and short-distance transport in animal cells. On the contrary, using melanosomes of melanocytes as a model, we recently discovered that the motor protein myosin-Va works with dynamic
Yi Li et al.
STAR protocols, 1(3), 100137-100137 (2020-12-31)
Alk-Ph is a clickable APEX2 substrate developed for spatially restricted protein/RNA labeling in intact yeast cells. Alk-Ph is more water soluble and cell wall permeable than biotin-phenol substrate, allowing more efficient profiling of the subcellular proteome in microorganisms. We describe
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