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Showing 1-30 of 47 results for "WGA1" within Papers
Stéphane Bertani et al.
Molecular cell, 43(6), 1040-1046 (2011-09-20)
The epigenetic activator Mixed lineage leukemia 1 (MLL1) is paramount for embryonic development and hematopoiesis. Here, we demonstrate that the long, noncoding RNA (lncRNA) Mistral (Mira) activates transcription of the homeotic genes Hoxa6 and Hoxa7 in mouse embryonic stem cells
Henriette O'Geen et al.
PLoS genetics, 3(6), e89-e89 (2007-06-05)
We performed a genome-scale chromatin immunoprecipitation (ChIP)-chip comparison of two modifications (trimethylation of lysine 9 [H3me3K9] and trimethylation of lysine 27 [H3me3K27]) of histone H3 in Ntera2 testicular carcinoma cells and in three different anatomical sources of primary human fibroblasts.
Ilona N Holcomb et al.
Cancer research, 69(19), 7793-7802 (2009-09-24)
Androgen deprivation is the mainstay of therapy for progressive prostate cancer. Despite initial and dramatic tumor inhibition, most men eventually fail therapy and die of metastatic castration-resistant (CR) disease. Here, we characterize the profound degree of genomic alteration found in
Suzanne E Little et al.
Genomics, 87(2), 298-306 (2005-11-08)
The ability to utilize formalin-fixed, paraffin-embedded (FFPE) archival specimens reliably for high-resolution molecular genetic analysis would be of immense practical application in the study of human disease. We have evaluated the ability of the GenomePlex whole genome amplification (WGA) kit
Andrei V Krivtsov et al.
Cancer cell, 14(5), 355-368 (2008-11-04)
We created a mouse model wherein conditional expression of an Mll-AF4 fusion oncogene induces B precursor acute lymphoblastic (ALL) or acute myeloid leukemias (AML). Gene expression profile analysis of the ALL cells demonstrated significant overlap with human MLL-rearranged ALL. ChIP-chip
Li Xu et al.
Nucleic acids research, 43(18), 8898-8912 (2015-09-20)
Cytokine or growth factor activated STAT3 undergoes multiple post-translational modifications, dimerization and translocation into nuclei, where it binds to serum-inducible element (SIE, 'TTC(N3)GAA')-bearing promoters to activate transcription. The STAT3 DNA binding domain (DBD, 320-494) mutation in hyper immunoglobulin E syndrome
Karin Wahlberg et al.
Blood, 114(6), 1254-1262 (2009-06-17)
HBS1L-MYB intergenic polymorphism (HMIP) on chromosome 6q23 is associated with elevated fetal hemoglobin levels and has pleiotropic effects on several hematologic parameters. To investigate potential regulatory activity in the region, we have measured sensitivity of the sequences to DNase I
Pete E Pascuzzi et al.
The Plant cell, 26(1), 102-120 (2014-02-04)
Scaffold or matrix attachment regions (S/MARs) are found in all eukaryotes. The pattern of distribution and genomic context of S/MARs is thought to be important for processes such as chromatin organization and modulation of gene expression. Despite the importance of
Alessandra Brambati et al.
Nucleic acids research, 46(3), 1227-1239 (2017-10-24)
The yeast RNA/DNA helicase Sen1, Senataxin in human, preserves the integrity of replication forks encountering transcription by removing RNA-DNA hybrids. Here we show that, in sen1 mutants, when a replication fork clashes head-on with transcription is arrested and, as a
Ravinder Dhallan et al.
Lancet (London, England), 369(9560), 474-481 (2007-02-13)
Use of free fetal DNA to diagnose fetal chromosomal abnormalities has been hindered by the inability to distinguish fetal DNA from maternal DNA. Our aim was to establish whether single nucleotide polymorphisms (SNPs) can be used to distinguish fetal DNA
Vladimir A Trifonov et al.
PloS one, 10(7), e0132380-e0132380 (2015-07-07)
Parthenogenesis, unisexuality and triploidy are interesting but poorly studied phenomena occurring in some reptile species. The mourning gecko (Lepidodactylus lugubris) represents a complex of diploid and triploid parthenogenetic mostly all-female populations (males occur quite rarely) widely distributed in coastal areas
Y R Thorstenson et al.
Genome research, 8(8), 848-855 (1998-09-02)
An automated, inexpensive, easy-to-use, and reproducible technique for controlled, random DNA fragmentation has been developed. The technique is based on point-sink hydrodynamics that result when a DNA sample is forced through a small hole by a syringe pump. Commercially available
Pei-Ching Chang et al.
Cancer research, 69(14), 5681-5689 (2009-07-09)
Kaposi's sarcoma-associated herpesvirus (KSHV) has been linked to the development of Kaposi's sarcoma, a major AIDS-associated malignancy, and to hematologic malignancies, including primary effusion lymphoma and multicentric Castleman's disease. Like other herpesviruses, KSHV is capable of both latent and lytic
António Pagarete et al.
Viruses, 7(7), 3937-3953 (2015-07-21)
Numbering in excess of 10 million per milliliter of water, it is now undisputed that aquatic viruses are one of the major factors shaping the ecology and evolution of Earth's microbial world. Nonetheless, environmental viral diversity and roles remain poorly
Agnieszka Maciejewska et al.
International journal of legal medicine, 127(2), 309-319 (2012-09-04)
Degraded DNA is often analyzed in forensic genetics laboratories. Reliable analysis of degraded DNA is of great importance, since its results impact the quality and reliability of expert testimonies. Recently, a number of whole genome amplification (WGA) methods have been
Huy Q Nguyen et al.
PLoS genetics, 11(2), e1005014-e1005014 (2015-02-28)
The spatial organization of chromosomes within interphase nuclei is important for gene expression and epigenetic inheritance. Although the extent of physical interaction between chromosomes and their degree of compaction varies during development and between different cell-types, it is unclear how
Carolyn M George et al.
Cells, 3(3), 865-882 (2014-08-26)
The 3-dimensional spatial organization of eukaryotic genomes is important for regulation of gene expression as well as DNA damage repair. It has been proposed that one basic biophysical property of all nuclei is that interphase chromatin must be kept in
Federico Ávila-Moreno et al.
PloS one, 9(12), e114104-e114104 (2014-12-03)
Lung cancer is the leading cause of death from malignant diseases worldwide, with the non-small cell (NSCLC) subtype accounting for the majority of cases. NSCLC is characterized by frequent genomic imbalances and copy number variations (CNVs), but the epigenetic aberrations
Whole genome amplification and genetic analysis after extraction of proteins from dried blood spots.
Mads V Hollegaard et al.
Clinical chemistry, 53(6), 1161-1162 (2007-05-23)
Li Jia et al.
PLoS genetics, 5(8), e1000597-e1000597 (2009-08-15)
Multiple discrete regions at 8q24 were recently shown to contain alleles that predispose to many cancers including prostate, breast, and colon. These regions are far from any annotated gene and their biological activities have been unknown. Here we profiled a
Susan Gribble et al.
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 12(2), 143-151 (2004-04-01)
We have used OmniPlex library technology to construct chromosome painting probes from single copies of flow sorted chromosomes. We show that this whole genome amplification technology is particularly efficient at amplifying single copies of chromosomes for the production of paints
Nazif Alic et al.
Molecular systems biology, 7, 502-502 (2011-06-23)
FoxO transcription factors, inhibited by insulin/insulin-like growth factor signalling (IIS), are crucial players in numerous organismal processes including lifespan. Using genomic tools, we uncover over 700 direct dFOXO targets in adult female Drosophila. dFOXO is directly required for transcription of
Pierangela Sabbattini et al.
The EMBO journal, 26(22), 4657-4669 (2007-10-20)
Combinatorial modifications of the core histones have the potential to fine-tune the epigenetic regulation of chromatin states. The Aurora B kinase is responsible for generating the double histone H3 modification tri-methylated K9/phosphorylated S10 (H3K9me3/S10ph), which has been implicated in chromosome
Joost F Swennenhuis et al.
Nucleic acids research, 40(3), e20-e20 (2011-11-30)
FISH probes are generally made out of BAC clones with genomic DNA containing a variable amount of repetitive DNA that will need to be removed or blocked for FISH analysis. To generate repeat free (RF) Probes without loss in genomic
Nicola A Johnson et al.
Laboratory investigation; a journal of technical methods and pathology, 86(9), 968-978 (2006-06-06)
Array-based comparative genomic hybridisation (aCGH) has diverse applications in cancer gene discovery and translational research. Currently, aCGH is performed primarily using high molecular weight DNA samples and its application to formalin-fixed and paraffin-embedded (FFPE) tissues remains to be established. To
Comparing Whole-Genome Amplification Methods and Sources for Biological Samples for Single-Nucleotide Polymorphism Genotyping
Park, J. W., et al.
Clinical Chemistry, 51(8), 1520-1523 (2004)
Artem P Lisachov et al.
Cytogenetic and genome research, 157(1-2), 115-122 (2019-03-02)
Pleurodont lizards are characterized by an ancient system of sex chromosomes. Along with stability of the central component of the system (homologous to the X chromosome of Anolis carolinensis [Dactyloidae], ACAX), in some genera the ancestral sex chromosomes are fused
Binding patterns of BCL11A in the globin and GATA1 loci and characterization of the BCL11A fetal hemoglobin locus
Jawaid K, et al.
Blood Cells, Molecules and Diseases, 45(2), 140-146 (2010)
Henriette O'Geen et al.
BioTechniques, 41(5), 577-580 (2006-12-05)
A single chromatin immunoprecipitation (ChIP) sample does not provide enough DNA for hybridization to a genomic tiling array. A commonly used technique for amplifying the DNA obtained from ChIP assays is ligation-mediated PCR (LM-PCR). However; using this amplification method, we
Daniel H Fine et al.
Journal of clinical microbiology, 45(12), 3859-3869 (2007-10-19)
Aggregatibacter actinomycetemcomitans is frequently associated with localized aggressive periodontitis (LAP); however, longitudinal cohort studies relating A. actinomycetemcomitans to initiation of LAP have not been reported. A periodontal assessment was performed on 1,075 primarily African-American and Hispanic schoolchildren, ages 11 to
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