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Showing 1-30 of 75 results for "WGA3" within Papers
Y R Thorstenson et al.
Genome research, 8(8), 848-855 (1998-09-02)
An automated, inexpensive, easy-to-use, and reproducible technique for controlled, random DNA fragmentation has been developed. The technique is based on point-sink hydrodynamics that result when a DNA sample is forced through a small hole by a syringe pump. Commercially available
Mythily Srinivasan et al.
The American journal of pathology, 161(6), 1961-1971 (2002-12-06)
Clinical and molecular medicines are undergoing a revolution based on the accelerated advances in biotechnology such as DNA microarrays and proteomics. Answers to fundamental questions such as how does the DNA sequence differ between individuals and what makes one individual
Colin R Lickwar et al.
Nature, 484(7393), 251-255 (2012-04-14)
Dynamic access to genetic information is central to organismal development and environmental response. Consequently, genomic processes must be regulated by mechanisms that alter genome function relatively rapidly. Conventional chromatin immunoprecipitation (ChIP) experiments measure transcription factor occupancy, but give no indication
Elizabeth P Murchison et al.
Cell, 148(4), 780-791 (2012-02-22)
The Tasmanian devil (Sarcophilus harrisii), the largest marsupial carnivore, is endangered due to a transmissible facial cancer spread by direct transfer of living cancer cells through biting. Here we describe the sequencing, assembly, and annotation of the Tasmanian devil genome
Kristen L Holler et al.
Developmental biology, 341(1), 291-304 (2010-02-11)
The basic helix-loop-helix DNA binding protein Hand2 has critical functions in cardiac development both in neural crest-derived and mesoderm-derived structures. Targeted deletion of Hand2 in the neural crest has allowed us to genetically dissect Hand2-dependent defects specifically in outflow tract
Delimiting the origin of a B chromosome by FISH mapping, chromosome painting and DNA sequence analysis in Astyanax paranae (Teleostei, Characiformes)
Duilio M Z, et al.
PLoS ONE, 9(4), e94896-e94896 (2014)
Susan M Gribble et al.
PloS one, 8(4), e60482-e60482 (2013-04-19)
Down syndrome (DS) is caused by trisomy of chromosome 21 (Hsa21) and presents a complex phenotype that arises from abnormal dosage of genes on this chromosome. However, the individual dosage-sensitive genes underlying each phenotype remain largely unknown. To help dissect
Diogo C Cabral-de-Mello et al.
Frontiers in genetics, 12, 661417-661417 (2021-04-17)
Tandem repeats are important parts of eukaryotic genomes being crucial e.g., for centromere and telomere function and chromatin modulation. In Lepidoptera, knowledge of tandem repeats is very limited despite the growing number of sequenced genomes. Here we introduce seven new
Érica Alves Serrano et al.
Comparative cytogenetics, 11(1), 81-95 (2017-09-19)
B chromosomes are apparently dispensable components found in the genomes of many species that are mainly composed of repetitive DNA sequences. Among the numerous questions concerning B chromosomes, the origin of these elements has been widely studied. To date, supernumerary
Alison P Sanders et al.
Epigenetics, 9(2), 212-221 (2013-10-31)
Cadmium (Cd) is prevalent in the environment yet understudied as a developmental toxicant. Cd partially crosses the placental barrier from mother to fetus and is linked to detrimental effects in newborns. Here we examine the relationship between levels of Cd
Xu Chen et al.
Cancer research, 72(9), 2294-2303 (2012-03-08)
NOTCH3 gene amplification plays an important role in the progression of many ovarian and breast cancers, but the targets of NOTCH3 signaling are unclear. Here, we report the use of an integrated systems biology approach to identify direct target genes
Mirella L Meyer-Ficca et al.
Chromosoma, 122(4), 319-335 (2013-06-05)
The mammalian sperm nucleus is characterized by unique properties that are important for fertilization. Sperm DNA retains only small numbers of histones in distinct positions, and the majority of the genome is protamine associated, which allows for extreme condensation and
Chao Bian et al.
Scientific reports, 6, 24501-24501 (2016-04-20)
The Asian arowana (Scleropages formosus), one of the world's most expensive cultivated ornamental fishes, is an endangered species. It represents an ancient lineage of teleosts: the Osteoglossomorpha. Here, we provide a high-quality chromosome-level reference genome of a female golden-variety arowana
K S Zadesenets et al.
Vavilovskii zhurnal genetiki i selektsii, 24(5), 519-524 (2021-03-05)
Application of microdissected DNA libraries and DNA probes in numerous and various modern molecular cytogenetic studies showed them as an efficient and reliable tool in the analysis of chromosome reorganization during karyotypic evolution and in the diagnosis of human chromosome
Eva Seifertova et al.
BMC genomics, 14, 357-357 (2013-05-30)
Genomic sequence assemblies are key tools for a broad range of gene function and evolutionary studies. The diploid amphibian Xenopus tropicalis plays a pivotal role in these fields due to its combination of experimental flexibility, diploid genome, and early-branching tetrapod
Tyrone Ryba et al.
Nature protocols, 6(6), 870-895 (2011-06-04)
Replication timing profiles are cell type-specific and reflect genome organization changes during differentiation. In this protocol, we describe how to analyze genome-wide replication timing (RT) in mammalian cells. Asynchronously cycling cells are pulse labeled with the nucleotide analog 5-bromo-2-deoxyuridine (BrdU)
Zhihao Ding et al.
PLoS genetics, 10(11), e1004798-e1004798 (2014-11-21)
Associating genetic variation with quantitative measures of gene regulation offers a way to bridge the gap between genotype and complex phenotypes. In order to identify quantitative trait loci (QTLs) that influence the binding of a transcription factor in humans, we
Martina Dalíková et al.
The Journal of heredity, 108(7), 709-719 (2017-10-11)
Moths and butterflies (Lepidoptera) represent the most diverse group of animals with heterogametic females. Although the vast majority of species has a WZ/ZZ (female/male) sex chromosome system, it is generally accepted that the ancestral system was Z/ZZ and the W
Theresia Lassak et al.
Molecular microbiology, 82(3), 602-618 (2011-09-20)
Efg1 is a central transcriptional regulator of morphogenesis and metabolism in Candida albicans. In vivo genome-wide ChIP chip and in vitro footprint analyses revealed the Efg1 recognition sequence (EGR-box) TATGCATA in the yeast growth form of this human fungal pathogen.
Eugen Dhimolea et al.
PloS one, 9(7), e99800-e99800 (2014-07-06)
Exposure to environmental estrogens (xenoestrogens) may play a causal role in the increased breast cancer incidence which has been observed in Europe and the US over the last 50 years. The xenoestrogen bisphenol A (BPA) leaches from plastic food/beverage containers
Vladimir Krylov et al.
Cytogenetic and genome research, 145(3-4), 192-200 (2015-05-30)
The genus Xenopus represents important model organisms in the field of developmental biology and chromosomal evolution. Developmental processes are tightly coupled with the analysis of gene function via genetic linkage and mapping. Cytogenetic techniques such as chromosome banding or FISH
Lucas Caetano de Barros et al.
Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation, 12(4), 204-209 (2018-06-08)
The W chromosome of Megaleporinus trifasciatus was isolated in order to analyze its behavior in the karyotype of this and other species of the family, including forms with differentiated and undifferentiated sex chromosomes. The chromosome was microdissected, and the WMt
Luis G Acevedo et al.
BioTechniques, 43(6), 791-797 (2008-02-07)
The technique of chromatin immunoprecipitation (ChIP) is a powerful method for identifying in vivo DNA binding sites of transcription factors and for studying chromatin modifications. Unfortunately, the large number of cells needed for the standard ChIP protocol has hindered the
Priscilla Cardim Scacchetti et al.
PloS one, 10(9), e0137231-e0137231 (2015-09-16)
Characidium constitutes an interesting model for cytogenetic studies, since a large degree of karyotype variation has been detected in this group, like the presence/absence of sex and supernumerary chromosomes and variable distribution of repetitive sequences in different species/populations. In this
Worapong Singchat et al.
PloS one, 11(8), e0160901-e0160901 (2016-08-09)
Genomic alteration in head and neck squamous cell carcinoma (HNSCC) was studied in two cell line pairs (HN30-HN31 and HN4-HN12) using conventional C-banding, multiplex fluorescence in situ hybridization (M-FISH), and array comparative genomic hybridization (array CGH). HN30 and HN4 were
Andrezza C S Bernardino et al.
Cytogenetic and genome research, 152(4), 213-221 (2017-10-11)
B chromosomes, extra elements present in the karyotypes of some eukaryote species, have been described in the grasshopper Xyleus discoideus angulatus. Although some studies have proposed an autosomal origin of the B chromosome in X. d. angulatus, little is known
Xoana Taboada et al.
Chromosoma, 123(3), 281-291 (2014-01-30)
Bacterial artificial chromosomes (BAC) have been widely used for fluorescence in situ hybridization (FISH) mapping of chromosome landmarks in different organisms, including a few in teleosts. In this study, we used BAC-FISH to consolidate the previous genetic and cytogenetic maps
Marcela B Pucci et al.
Cytogenetic and genome research, 149(3), 182-190 (2016-11-03)
Sex chromosome evolution involves the accumulation of repeat sequences such as multigenic families, noncoding repetitive DNA (satellite, minisatellite, and microsatellite), and mobile elements such as transposons and retrotransposons. Most species of Characidium exhibit heteromorphic ZZ/ZW sex chromosomes; the W is
Sally Yoo et al.
BMC genomics, 15, 724-724 (2014-08-29)
Aberrational epigenetic marks are believed to play a major role in establishing the abnormal features of cancer cells. Rational use and development of drugs aimed at epigenetic processes requires an understanding of the range, extent, and roles of epigenetic reprogramming
K S Zadesenets et al.
Vavilovskii zhurnal genetiki i selektsii, 24(6), 636-642 (2021-03-05)
The free-living flatworm Macrostomum mirumnovem is a neopolyploid species whose genome underwent a recent Whole Genome Duplication (WGD). In the result of chromosome fusions of the ancient haploid chromosome set, large metacentric chromosomes were formed. In addition to three pairs
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