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Showing 1-30 of 203 results for "WGA4" within Papers
DaYang Chen et al.
Scientific reports, 8(1), 4963-4963 (2018-03-23)
Research based on a strategy of single-cell low-coverage whole genome sequencing (SLWGS) has enabled better reproducibility and accuracy for detection of copy number variations (CNVs). The whole genome amplification (WGA) method and sequencing platform are critical factors for successful SLWGS
Susan Gribble et al.
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 12(2), 143-151 (2004-04-01)
We have used OmniPlex library technology to construct chromosome painting probes from single copies of flow sorted chromosomes. We show that this whole genome amplification technology is particularly efficient at amplifying single copies of chromosomes for the production of paints
Eunhee Choi et al.
Cell, 166(3), 567-581 (2016-07-05)
Insulin signaling regulates many facets of animal physiology. Its dysregulation causes diabetes and other metabolic disorders. The spindle checkpoint proteins MAD2 and BUBR1 prevent precocious chromosome segregation and suppress aneuploidy. The MAD2 inhibitory protein p31(comet) promotes checkpoint inactivation and timely
Jochen B Geigl et al.
Nature protocols, 2(12), 3173-3184 (2007-12-15)
A comprehensive genomic analysis of single cells is instrumental for numerous applications in tumor genetics, clinical diagnostics and forensic analyses. Here, we provide a protocol for single-cell isolation and whole genome amplification, which includes the following stages: preparation of single-cell
Young Chung et al.
Cloning and stem cells, 11(2), 213-223 (2009-02-04)
There is renewed interest in using animal oocytes to reprogram human somatic cells. Here we compare the reprogramming of human somatic nuclei using oocytes obtained from animal and human sources. Comparative analysis of gene expression in morula-stage embryos was carried
Kira S Zadesenets et al.
Scientific reports, 7(1), 6066-6066 (2017-07-22)
The free-living flatworm Macrostomum lignano is a model organism for evolutionary and developmental biology studies. Recently, an unusual karyotypic diversity was revealed in this species. Specifically, worms are either 'normal' 2n = 8, or they are aneuploid with one or two additional
Christopher E Barbieri et al.
Nature genetics, 44(6), 685-689 (2012-05-23)
Prostate cancer is the second most common cancer in men worldwide and causes over 250,000 deaths each year. Overtreatment of indolent disease also results in significant morbidity. Common genetic alterations in prostate cancer include losses of NKX3.1 (8p21) and PTEN
Abdul Hakim Elnfati et al.
Genomics data, 7, 175-177 (2016-03-17)
During spermiogenesis in mammals and many other vertebrate classes, histone-containing nucleosomes are replaced by protamine toroids, which can repackage chromatin at a 10 to 20-fold higher density than in a typical somatic nucleus. However, recent evidence suggests that sperm of
Sherif El-Sharnouby et al.
PLoS genetics, 9(10), e1003842-e1003842 (2013-10-23)
The Drosophila spermatogenesis cell differentiation pathway involves the activation of a large set of genes in primary spermatocytes. Most of these genes are activated by testis-specific TATA-binding protein associated factors (tTAFs). In the current model for the activation mechanism, Polycomb
Eric J Forman et al.
Fertility and sterility, 98(3), 644-649 (2012-05-23)
To assess the impact of oocyte vitrification on aneuploidy and reproductive potential by comparing vitrified and control oocytes from a single patient within a single cycle and a single fresh transfer. Paired randomized controlled trial in which each patient's cohort
José Carlos Pansonato-Alves et al.
PloS one, 9(9), e107169-e107169 (2014-09-17)
Chromosome painting with DNA probes obtained from supernumerary (B) and sex chromosomes in three species of fish genus Characidium (C. gomesi, C. pterostictum and C. oiticicai) showed a close resemblance in repetitive DNA content between B and sex chromosomes in
Suk See De Ravin et al.
Nature communications, 13(1), 3710-3710 (2022-06-29)
X-linked Severe Combined Immunodeficiency (SCID-X1) due to IL2RG mutations is potentially fatal in infancy where 'emergency' life-saving stem cell transplant may only achieve incomplete immune reconstitution following transplant. Salvage therapy SCID-X1 patients over 2 years old (NCT01306019) is a non-randomized, open-label
Yan Guo et al.
International journal of cancer, 136(12), 2973-2979 (2014-11-19)
Intestinal metaplasia (IM) has been recognized as the first irreversible precancerous stage of intestinal-type gastric cancer at which genetic instabilities, such as microsatellite (MS) instability and loss of heterozygosity, can already be detected. However, the extent and clonal relationship of
Dehua Cheng et al.
Journal of assisted reproduction and genetics, 38(1), 243-250 (2020-10-24)
To elucidate the genetic cause of intellectual deficiency and/or congenital malformations in two parental reciprocal translocation carriers and provide appropriate strategies of assisted reproductive therapy (ART). Two similar couples having a child with global developmental delay/intellectual disability symptoms attended the
Kunio Kitada et al.
Genes, chromosomes & cancer, 50(4), 217-227 (2011-02-15)
Analysis of structural rearrangements at the individual chromosomal level is still technologically challenging. Here we optimized a chromosome isolation method using fluorescent marker-assisted laser-capture and laser-beam microdissection and applied it to structural analysis of two aberrant chromosomes found in a
Marta N Shahbazi et al.
Nature communications, 11(1), 3987-3987 (2020-08-12)
Aneuploidy, the presence of an abnormal number of chromosomes, is a major cause of early pregnancy loss in humans. Yet, the developmental consequences of specific aneuploidies remain unexplored. Here, we determine the extent of post-implantation development of human embryos bearing
Juliette Auvinet et al.
BMC evolutionary biology, 20(1), 39-39 (2020-03-21)
Chromosomal rearrangements are thought to be an important driving force underlying lineage diversification, but their link to speciation continues to be debated. Antarctic teleost fish of the family Nototheniidae (Notothenioidei) diversified in a changing environmental context, which led to ecological
Siri Malmgren et al.
The Journal of biological chemistry, 288(17), 11973-11987 (2013-03-12)
Lipotoxicity is a presumed pathogenetic process whereby elevated circulating and stored lipids in type 2 diabetes cause pancreatic β-cell failure. To resolve the underlying molecular mechanisms, we exposed clonal INS-1 832/13 β-cells to palmitate for 48 h. We observed elevated
Daniel Stieber et al.
Acta neuropathologica, 127(2), 203-219 (2013-10-25)
Glioblastoma (GBM) is known to be a heterogeneous disease; however, the genetic composition of the cells within a given tumour is only poorly explored. In the advent of personalised medicine the understanding of intra-tumoural heterogeneity at the cellular and the
Jia-Hui Ng et al.
Developmental cell, 24(3), 324-333 (2013-01-29)
The limited number of in vivo germ cells poses an impediment to genome-wide studies. Here, we applied a small-scale chromatin immunoprecipitation sequencing (ChIP-seq) method on purified mouse fetal germ cells to generate genome-wide maps of four histone modifications (H3K4me3, H3K27me3
Davide F Robbiani et al.
Molecular cell, 36(4), 631-641 (2009-11-28)
Cancer-initiating translocations such as those associated with lymphomas require the formation of paired DNA double-strand breaks (DSBs). Activation-induced cytidine deaminase (AID) produces widespread somatic mutation in mature B cells; however, the extent of "off-target" DSB formation and its role in
Wen Fong Ooi et al.
Nature communications, 7, 12983-12983 (2016-09-30)
Regulatory enhancer elements in solid tumours remain poorly characterized. Here we apply micro-scale chromatin profiling to survey the distal enhancer landscape of primary gastric adenocarcinoma (GC), a leading cause of global cancer mortality. Integrating 110 epigenomic profiles from primary GCs
Sara Franceschi et al.
Oncotarget, 9(35), 24014-24027 (2018-05-31)
Glioblastoma is a devastating disease that despite all the information gathered so far, its optimal management remains elusive due to the absence of validated targets from clinical studies. A better clarification of the molecular mechanisms is needed. In this study
Nathan R Treff et al.
Molecular human reproduction, 17(6), 335-343 (2010-12-24)
Methods of comprehensive microarray-based aneuploidy screening in single cells are rapidly emerging. Whole-genome amplification (WGA) remains a critical component for these methods to be successful. A number of commercially available WGA kits have been independently utilized in previous single-cell microarray
Ping Yuan et al.
Journal of assisted reproduction and genetics, 38(3), 709-718 (2021-01-08)
The aim of this study was to determine factors affecting the chromosome imbalance in blastocysts and reproductive outcomes by a comparison between the reciprocal translocation (REC), inversion (INV), and Robertsonian translocation (ROB) carriers. Couples with one partner carrying translocation or
Tatyana Karamysheva et al.
Cells, 10(7) (2021-08-08)
The gene composition, function and evolution of B-chromosomes (Bs) have been actively discussed in recent years. However, the additional genomic elements are still enigmatic. One of Bs mysteries is their spatial organization in the interphase nucleus. It is known that
Elisabeth Ullrich et al.
Journal of biotechnology, 310, 80-88 (2020-02-06)
We have identified 24 molecular markers, based on circulating nucleic acids (CNA) originating from the human genome, which in combination can be used in a quantitative real-time PCR (qPCR) assay to identify the presence of human sepsis, starting two to
Norma J Nowak et al.
Genetics in medicine : official journal of the American College of Medical Genetics, 9(9), 585-595 (2007-09-18)
To address some of the challenges facing the incorporation of array comparative genomic hybridization technology as a clinical tool, including archived tumor tissue, tumor heterogeneity, DNA quality and quantity, and array comparative genomic hybridization platform selection and performance. Experiments were
Santosh Gupta et al.
Molecular cancer research : MCR, 19(6), 1040-1050 (2021-03-28)
Men with circulating tumor cell (CTC) AR-V7-positive metastatic castration-resistant prostate cancer (mCRPC) have worse outcomes when treated with enzalutamide/abiraterone. However, most men lack CTC AR-V7 detection, and additional predictive biomarkers are needed. We conducted a retrospective secondary analysis of the
Tamir Biezuner et al.
Scientific reports, 11(1), 17171-17171 (2021-08-27)
Advances in whole genome amplification (WGA) techniques enable understanding of the genomic sequence at a single cell level. Demand for single cell dedicated WGA kits (scWGA) has led to the development of several commercial kit. To this point, no robust
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