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Showing 1-15 of 15 results for "Z722553SIGMA" within Site Content
Reverse Transcription Protocol Using SYBR Green Dye Detection
Perform reverse transcription (RT) using a reverse transcriptase enzyme and dNTPs. Use total RNA or a gene-specific approach so that only the RNA of interest is converted to cDNA.
Multiplex qPCR Protocol
Standard qPCR protocol with up to four detection probes at specified concentrations, simplifying reaction setup with LuminoCt ReadyMix.
qPCR with Single Detection Probe
qPCR protocol template with specific detection probes aids in single-target detection using LuminoCt® ReadyMix™.
Standard Reverse Transcription Protocol
Reverse transcription analyzes mRNA gene expression, facing challenges like transcript half-life differences and temporal patterns.
Primer Concentration Optimization
Primer Concentration Optimization Protocol creates reaction matrix for testing primer concentrations against various partners.
KiCqStart™ Universal SYBR® Green qPCR Protocol
Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.
Primer Optimization Using Temperature Gradient
Gradient PCR optimizes assay conditions by testing fixed primer concentrations across various annealing temperatures.
qPCR Reference Gene Selection Protocol
Analysis of gene expression data requires a stable reference or loading control. This reference is usually one or more reference genes.
qPCR Gene Expression Protocol Using SYBR Green
SYBR Green I dye in qPCR measures target quantity, adaptable to specific needs with a standard protocol.
The 3'/5' Assay for Analysis of RNA Integrity Protocol
The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.
qPCR Efficiency Determination Protocol
Optimization of qPCR conditions is important for the development of a robust assay. The two main approaches are optimization of primer concentration and/or annealing temperatures.
SYBR® Green I Dye Quantitative PCR Protocol
A protocol that can be used as a basic template for qPCR incorporating SYBR Green I DNA binding dye that is amenable to modification and applicable for use as validation for a set of primers.
SPUD Assay for Detection of Assay Inhibitors Protocol
SPUD assay identifies inhibitors in RNA or DNA samples, useful for analyzing numerous or low-copy targets.
Universal SYBR Green qPCR Protocol
Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions
MystiCq® MicroRNA® Quantitation System
Method for purification, reverse transcription and quantitative PCR for MicroRNAs using Mysticq reagents