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Detergents Properties and Applications
25
6)
ZWITTERIONIC NON-DETERGENT SULFOBETAINES
Non-detergent Sulfobetaines Although not detergents, these reagents possess hydrophilic groups similar to
those of zwitterionic detergents but with
Product Information Sheet - PROTDT
-4
When selecting a detergent, the first consideration is
usually the ionic form of the hydrophilic group, which is
either ionic, zwitterionic, or non-ionic. Ionic detergents
typically modify protein
C7BzO: A Powerful New Detergent for 2-D Gel Electrophoresis Sample Preparation
analysis. This new detergent should be a useful
and powerful protein extraction tool in a variety of pro-
teomic applications.
References
1. Chevallet, M., et al., New zwitterionic detergents improve the analysis
Product Information Sheet - D4641
crystallized, water soluble, nonionic
detergent similar to n-octyl-β-D-glucoside. In studies
on rhodopsin and opsin in different nonionic and
zwitterionic detergents, this detergent was superior in
solubility
Detergent Optimization for Total Protein Extraction from Chinese Hamster Ovary (CHO) Cells
culture. Zwitterionic detergents were chosen
for the total protein extraction based on published research
describing successful solubilization of membrane proteins [2].
While not every detergent supplied
Product Information Sheet - C9426
337-343 (1991).
ProductInformation
2
6. Perdew, G.H., et al., The use of a zwitterionic
detergent in two-dimensional gel electrophoresis of
trout liver microsomes. Anal. Biochem., 135, 453
CALBIOSORBﾙ Technical Bulletin
-used detergents.
Detergent Cat. No. M.W. Type Adsorption Capacity
(mg detergent/ml resin)
Cetyltrimethylammonium Bromide (CTAB) 219375 364.5 Cationic 120
CHAPS 220201 614.9 Zwitterionic 110
Product Information - C5849
337-343 (1991).
ProductInformation
2
6. Perdew, G.H., et al., The use of a zwitterionic
detergent in two-dimensional gel electrophoresis of
trout liver microsomes. Anal. Biochem., 135, 453
Detergents Booklet
.
�0
Zwitterionic Detergents
Zwitterionic detergents are unique in that they offer the combined properties of
ionic and non-ionic detergents. Like non-ionic detergents the zwittergents
Monitoring detergent removal from biological samples using the Direct Detect®spectrometer
monitoring of
detergent removal efficiency can ensure that a sample
is sufficiently free of detergents to enable successful
downstream analysis.
In the set of detergents investigated, a zwitterionic CHAPS
The Deviron® Detergent Portfolio
Emprove® Evolve
Detergent
Deviron® 13-S9 Emprove®
Expert Detergent
Triton™ X-100 Emprove® Expert
Detergent
Product code 108693 108694 108643
Surfactant type Zwitterionic (pI 8.9) Non-ionic
Product Information Sheet - MAK459
optical density (OD) at 560 nm for
Cationic/Nonionic Detergents or at 650 nm
for Anionic Detergents.
Results
1. Subtract the No Detergent Standard OD
value (Water in the Standard curve) from
Product Information Sheet - L2274
autoclave lauryl
sulfate solutions.
References
1. Perdew, G. H., et al., The use of a zwitterionic
detergent in two-dimensional gel electrophoresis
of trout liver microsomes. Anal. Biochem., 135
Data Sheet - E7642
reduced by addition of 0.1 M
2-mercaptoethanol. In addition, detergents such as
Triton X-100, n-octylglucoside, or zwitterionic
detergents have no influence on the enzyme activity.
References
1. Parekh
Molecular Biology Grade Reagents
Type of
Detergent
Ionic Detergents Non-Ionic Detergents Zwitterionic Detergents
Examples
Anionic:
Sodium dodecyl sulfate (SDS)
Cationic:
Cetyl methyl ammonium bromide (CTAB)
TRITON®-X-
Product Information Sheet - C8740
Product Description
CelLytic B 10× is a proprietary, non-denaturing
formulation of zwitterionic detergents used for the lysis
of bacterial cells and extraction of recombinant proteins.
CelLytic
Product Information Sheet - C0606
is one of the traditional
combinations available for 2D electrophoresis and
contains zwitterionic detergents. To improve the
separation of proteins and reduce artifacts on an
isoelectric focusing
Product Information Sheet - TT0010
proteins. The ProteoPrep Detergent Sample
Kit (Product Code PROTDT) contains 10 non-ionic and
zwitterionic detergents suitable for protein solubilization.
One or more of these detergents may be incorporated
TB301 Reportasol Extraction Buffer
preservation of extremely high reporter enzyme activity. A proprietary mixture of non-ionic and
zwitterionic detergents, Reportasol disrupts the plasma membrane, leading to release of soluble
reporter enzymes
Product Information Sheet - ENDOHRO
highly increased
by addition of 0.1 M 2-mercaptoethanol.
• Detergents such as Triton X-100*, n-Octylglucoside*, or zwitterionic detergents show no influence on the enzyme
activity.
• In general, 50
Product Information Sheet - C0731
powerful solubilizing combinations available
for 2D electrophoresis and contains a novel zwitterionic
detergent, ASB-14. To improve the separation of
proteins and reduce artifacts on an isoelectric focusing
Product Information Sheet - C0481
is one of the traditional
combinations available for 2D electrophoresis and
contains a zwitterionic detergent. To improve the
separation of proteins and reduce artifacts on an
isoelectric focusing
Product Information Sheet - C0356
powerful solubilizing combination available for
2D electrophoresis and contains a novel zwitterionic
detergent, C7BzO. To improve the separation of
proteins and reduce artifacts on an isoelectric focusing
Product Information Sheet - B7310
CelLytic B 2X contains 40 mM Tris-HCl, pH 8.0 and a
proprietary, non-denaturing formulation of zwitterionic
detergents. CelLytic B 2X is different from CelLytic B in
that it provides a more concentrated protein
Product Information Sheet - CB0050
CelLytic B contains 40 mM Tris-HCl, pH 8.0 and a
proprietary, non-denaturing formulation of zwitterionic
detergents. There is no need for special equipment to
disrupt cells such as a sonicator or French
Product Information Sheet - CB0500
CelLytic B contains 40 mM Tris-HCl, pH 8.0 and a
proprietary, non-denaturing formulation of zwitterionic
detergents. There is no need for special equipment to
disrupt cells such as a sonicator or French
Product Information Sheet - B7435
B contains 40 mM Tris-HCl, pH 8.0, and a
proprietary, non-denaturing formulation of
zwitterionic detergents. There is no need for special
equipment to disrupt cells such as a sonicator or
French
LC-MS Contaminants: Avoid, identify, mimimize.
C2H4O]n Triton® 101 Detergents
381.2981 [AB3+Na]+ [C15H30O][C2H4O]n Triton®, reduced 101R Detergents
383.2797 [AB4+H]+ [C14H22O][C2H4O]n Triton® X-100, X-114, X-405, or X-45 Detergents
388.1284 [M+NH4]+
Brochure: LC-MS Contaminants
C2H4O]n Triton® 101 Detergents
381.2981 [AB3+Na]+ [C15H30O][C2H4O]n Triton®, reduced 101R Detergents
383.2797 [AB4+H]+ [C14H22O][C2H4O]n Triton® X-100, X-114, X-405, or X-45 Detergents
388.1284 [M+NH4]+
Solutions for Mass Spectrometry
plasticizer (from detergent bottle)
14
9.
1
20
5.
1
30
1.
3
39
9.
4
48
7.
3
57
9.
1
00 400 800 8001200 1600 m/z
dishwasher 2*
*
Triton detergents
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