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  • β-Hydroxybutyrate Elicits Favorable Mitochondrial Changes in Skeletal Muscle.

β-Hydroxybutyrate Elicits Favorable Mitochondrial Changes in Skeletal Muscle.

International journal of molecular sciences (2018-08-04)
Brian A Parker, Chase M Walton, Sheryl T Carr, Jacob L Andrus, Eric C K Cheung, Michael J Duplisea, Esther K Wilson, Carrie Draney, Daniel R Lathen, Kyle B Kenner, David M Thomson, Jeffery S Tessem, Benjamin T Bikman
ABSTRACT

The clinical benefit of ketosis has historically and almost exclusively centered on neurological conditions, lending insight into how ketones alter mitochondrial function in neurons. However, there is a gap in our understanding of how ketones influence mitochondria within skeletal muscle cells. The purpose of this study was to elucidate the specific effects of β-hydroxybutyrate (β-HB) on muscle cell mitochondrial physiology. In addition to increased cell viability, murine myotubes displayed beneficial mitochondrial changes evident in reduced H₂O₂ emission and less mitochondrial fission, which may be a result of a β-HB-induced reduction in ceramides. Furthermore, muscle from rats in sustained ketosis similarly produced less H₂O₂ despite an increase in mitochondrial respiration and no apparent change in mitochondrial quantity. In sum, these results indicate a general improvement in muscle cell mitochondrial function when β-HB is provided as a fuel.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Sigma-Aldrich
(±)-Sodium 3-hydroxybutyrate, ≥99.0% (NT)