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  • N-acetyl-D-glucosamine medium improves recovery of Haemophilus influenzae from sputa of patients with cystic fibrosis.

N-acetyl-D-glucosamine medium improves recovery of Haemophilus influenzae from sputa of patients with cystic fibrosis.

Journal of clinical microbiology (1993-07-01)
L V Möller, L van Alphen, H Grasselier, J Dankert
ABSTRACT

A modified selective medium supplemented with N-acetyl-D-glucosamine (NAG), hemin, and NAD plus two cefsulodin disks, for primary isolation of nonencapsulated Haemophilus influenzae from sputum of patients with cystic fibrosis, is described. Isolation of H. influenzae from this medium, designated NAG medium, was compared with recovery by standard media and immunochemical detection of H. influenzae with monoclonal antibody 8BD9. The H. influenzae recovery rate increased from 31% with standard media to 42% with NAG medium. H. influenzae was detected by immunoperoxidase staining in 54% of the sputum specimens. The results of this study demonstrate that NAG medium improves H. influenzae recovery, although immunoperoxidase staining is superior for detection of H. influenzae from sputum of cystic fibrosis patients.

MATERIALS
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Product Description

Millipore
N-Acetyl-D-glucosamine, An acceptor substrate for galactosyltransferases. Inhibits the lectin WGA and β-N-acetylhexosaminidase (Ki = 430 µM). When added to a medium, it improves the recovery of Haemophilus influenza from sputum.