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  • Facile generation of a biotechnologically-relevant catalase showcases the efficacy of a blue-green algal biomass as a suitable bioresource for protein overproduction.

Facile generation of a biotechnologically-relevant catalase showcases the efficacy of a blue-green algal biomass as a suitable bioresource for protein overproduction.

Bioresource technology (2019-09-09)
Dhiman Chakravarty, Manisha Banerjee, Anand Ballal
ABSTRACT

Here, we show the utility of a cyanobacterial biomass for overproduction and easy downstream processing of the thermostable protein KatB (a Mn-catalase). The nitrogen-fixing blue-green alga, Anabaena, was bioengineered to overexpress the KatB protein (An-KatB). Interestingly, pure An-KatB could be isolated from Anabaena by a simple physical process, obviating the need of expensive resins or chromatographic steps. An-KatB was an efficient H2O2-detoxifying protein that retained all the properties of Mn-catalases. Surprisingly, the purified An-KatB showed improved characteristics than the corresponding KatB (Ec-KatB) protein purified after over-expression in E. coli. An-KatB was unaffected by exposure to high temperature (85 °C), whereas a commercially procured heme-catalase showed an appreciable drop in activity beyond 50 °C. These data convincingly demonstrate the utility of Anabaena as a competent microbial bioresource for overproduction of proteins and further highlight the advantage of An-KatB over heme-catalases in bioprocesses where H2O2 is to be decomposed at elevated temperatures.

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Peroxide Assay Kit, sufficient for 250 colorimetric tests