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  • Axon-like protrusions promote small cell lung cancer migration and metastasis.

Axon-like protrusions promote small cell lung cancer migration and metastasis.

eLife (2019-12-14)
Dian Yang, Fangfei Qu, Hongchen Cai, Chen-Hua Chuang, Jing Shan Lim, Nadine Jahchan, Barbara M Grüner, Christin S Kuo, Christina Kong, Madeleine J Oudin, Monte M Winslow, Julien Sage
ABSTRACT

Metastasis is the main cause of death in cancer patients but remains a poorly understood process. Small cell lung cancer (SCLC) is one of the most lethal and most metastatic cancer types. SCLC cells normally express neuroendocrine and neuronal gene programs but accumulating evidence indicates that these cancer cells become relatively more neuronal and less neuroendocrine as they gain the ability to metastasize. Here we show that mouse and human SCLC cells in culture and in vivo can grow cellular protrusions that resemble axons. The formation of these protrusions is controlled by multiple neuronal factors implicated in axonogenesis, axon guidance, and neuroblast migration. Disruption of these axon-like protrusions impairs cell migration in culture and inhibits metastatic ability in vivo. The co-option of developmental neuronal programs is a novel molecular and cellular mechanism that contributes to the high metastatic ability of SCLC.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-UCHL1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-Microtubule-Associated Protein 2 (MAP2) Antibody, Chemicon®, from rabbit