• Early weaning induces short- and long-term effects on pancreatic islets in Wistar rats of both sexes.

Early weaning induces short- and long-term effects on pancreatic islets in Wistar rats of both sexes.

The Journal of physiology (2019-12-13)
Carla Bruna Pietrobon, Rosiane Aparecida Miranda, Iala Milene Bertasso, Paulo Cezar de Freitas Mathias, Maria Lúcia Bonfleur, Sandra Lucinei Balbo, Marise Auxiliadora de Barros Reis, Márcia Queiroz Latorraca, Vanessa Cristina Arantes, Elaine de Oliveira, Patrícia Cristina Lisboa, Egberto Gaspar de Moura

The World Health Organization recommends exclusive breastfeeding until 6 months of age as an important strategy to reduce child morbidity and mortality. Studies have associated early weaning with the development of obesity and type 2 diabetes in adulthood. In our model, we demonstrated that early weaning leads to increased insulin secretion in adolescent males and reduced insulin secretion in adult offspring. Early weaned males exhibit insulin resistance in skeletal muscle. Early weaning did not change insulin signalling in the muscle of female offspring. Taking into account that insulin resistance is one of the primary factors for the development of type 2 diabetes mellitus, this work demonstrates the importance of breastfeeding in the fight against this disease. Early weaning (EW) leads to short- and long-term obesity and diabetes. This phenotype is also observed in experimental models, in which early-weaned males exhibit abnormal insulinaemia in adulthood. However, studies regarding the effect of EW on pancreatic islets are rare. We investigated the mechanisms by which glycaemic homeostasis is altered in EW models through evaluations of insulin secretion and its signalling pathway in offspring. Lactating Wistar rats and their pups were divided into the following groups: non-pharmacological EW (NPEW): mothers were wrapped with an adhesive bandage on the last 3 days of lactation; pharmacological EW (PEW): mothers received bromocriptine to inhibit prolactin (1 mg/kg body mass/day) on the last 3 days of lactation; and control (C): pups underwent standard weaning at PN21. Offspring of both sexes were euthanized at PN45 and PN180. At PN45, EW males showed higher insulin secretion (vs. C). At PN170, PEW males exhibited hyperglycaemia in an oral glucose tolerance test (vs. C and NPEW). At PN180, EW male offspring were heavier; however, both sexes showed higher visceral fat. Insulin secretion was lower in EW offspring of both sexes. Males from both EW groups had lower glucokinase in islets, but unexpectedly, PEW males showed higher GLUT2, than did C. EW males exhibited lower insulin signalling in muscle. EW females exhibited no changes in these parameters compared with C. We demonstrated distinct alterations in the insulin secretion of EW rats at different ages. Despite the sex dimorphism in insulin secretion in adolescence, both sexes showed impaired insulin secretion in adulthood due to EW.

Product Number
Product Description

Anti-Rabbit IgG (H+L), CF 555 antibody produced in goat, ~2 mg/mL, affinity isolated antibody
DL-Glyceraldehyde 3-phosphate solution, 45-55 mg/mL in H2O
Anti-GLUT4 (C-terminal) antibody produced in rabbit, ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Anti-Estrogen Receptor α Antibody, clone F3-A, ascites fluid, clone F3-A, from mouse