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  • Bellymount enables longitudinal, intravital imaging of abdominal organs and the gut microbiota in adult Drosophila.

Bellymount enables longitudinal, intravital imaging of abdominal organs and the gut microbiota in adult Drosophila.

PLoS biology (2020-01-28)
Leslie Ann Jaramillo Koyama, Andrés Aranda-Díaz, Yu-Han Su, Shruthi Balachandra, Judy Lisette Martin, William B Ludington, Kerwyn Casey Huang, Lucy Erin O'Brien
ABSTRACT

Cell- and tissue-level processes often occur across days or weeks, but few imaging methods can capture such long timescales. Here, we describe Bellymount, a simple, noninvasive method for longitudinal imaging of the Drosophila abdomen at subcellular resolution. Bellymounted animals remain live and intact, so the same individual can be imaged serially to yield vivid time series of multiday processes. This feature opens the door to longitudinal studies of Drosophila internal organs in their native context. Exploiting Bellymount's capabilities, we track intestinal stem cell lineages and gut microbial colonization in single animals, revealing spatiotemporal dynamics undetectable by previously available methods.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sucrose, BioUltra, for molecular biology, ≥99.5% (HPLC)
Whatman® qualitative filter paper, Grade 2, circles, diam. 110 mm, pack of 100
Grace Bio-Labs SecureSeal adhesive sheets, thickness 0.48 mm , double sided, size 7.25 in. × 10 in.
Sigma-Aldrich
Chloramphenicol, Chloramphenicol, CAS 56-75-7, is a synthetic bacteriostatic antibiotic that inhibits the translation of RNA by blocking the peptidyltransferase reaction on ribosomes.
Sigma-Aldrich
Schneider′s Insect Medium, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for insect cell culture