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  • Simultaneous determination of phosphatidylglycerol and the lecithin/sphingomyelin ratio in amniotic fluid.

Simultaneous determination of phosphatidylglycerol and the lecithin/sphingomyelin ratio in amniotic fluid.

Clinical chemistry (1978-07-01)
G R Gotelli, R E Stanfill, P M Kabra, F A Farina, L J Marton
PMID77745
ABSTRACT

We describe a single-dimension thin-layer chromatographic method by which one can simultaneously determine the lecithin/sphingomyelin ratio and the proportion of phosphatidylglycerol in amniotic fluid. The phospholipids are conveniently detected by an improved molybdenum-blue spray reagent, which immediately produces blue spots on a white background. The stained phospholipids are stable for at least 90 min after spraying, and densitometry results in symmetrical peaks that are easily quantitated.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Molybdenum Blue spray reagent