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  • Inhibition of the synthesis of eicosanoid-like substances in a human oral cancer cell line by interferon-gamma and eicosapentaenoic acid.

Inhibition of the synthesis of eicosanoid-like substances in a human oral cancer cell line by interferon-gamma and eicosapentaenoic acid.

Archives of oral biology (1999-01-07)
C L Meng, C Y Yang, K L Shen, P Y Wong, H K Lee
ABSTRACT

The objectives were to examine the production of eicosanoids in a Chinese human oral cancer cell line (OEC-M1) and to test the effects of interferon-gamma (IFN-gamma), eicosapentaenoic acid (EPA) and enzyme inhibitors on this biosynthesis. The eicosanoids were identified by reverse phase-high performance liquid chromatography. Two predominant peaks appeared in the chromatograms. One compound (P-1) was identified by ultraviolet absorption at a lambda(max) of 278nm with shoulders at 272 and 284nm. The other compound (P-2) was identified by ultraviolet absorption at a lambda(max) of 284 nm with shoulders at 278 and 290 nm. The production of P- was significantly inhibited by the addition of IFN-gamma (200 and 400 U/ml), and EPA (10 to 40 microM). It was only partially inhibited (p < 0.05) by indomethacin (INDO) (0.5 and 1 microM), nordihydroguaiaretic acid (NDGA) (30 and 60 microM/ml), and eicosa-5,8,11,14-tetraynoic acid (ETYA) (20-60 microM). It was almost completely inhibited by indomethacin (2 and 3 microM), and dexamethasone (0.6 and 6 microM). The production of P-2 was almost completely inhibited by IFN-gamma (200 and 400 U/ml), and partially inhibited (p < 0.05) by EPA (10 and 20 microM), NDGA (30 and 60 microM), ETYA (20 and 40 microM), dexamethasone (0.6 and 6 microM). The production of both peaks was significantly reduced by excluding arachidonic acid (AA), and almost completely inhibited by heating at 100 degrees C for 10 min during incubation. These results demonstrate that two eicosanoid-like compounds are synthesized by the OEC-M cell line and that their production can be modulated by IFN-gamma, EPA, indomethacin, NDGA, ETYA, and dexamethasone.

MATERIALS
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Product Description

Sigma-Aldrich
OECM-1 Human Oral Squamous Carcinoma Cell Line, OECM-1 human oral squamous carcinoma cell line is suitable for studies of cancer cell signaling, epithelial-mesenchymal transition (EMT), metastasis, invasion, and cancer cell stemness.