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  • Identification of Lathyrus sativus plant volatiles causing behavioral preference of Aphis craccivora.

Identification of Lathyrus sativus plant volatiles causing behavioral preference of Aphis craccivora.

Pest management science (2020-07-23)
Paroma Mitra, Swati Das, Rahul Debnath, Syed Husne Mobarak, Anandamay Barik
ABSTRACT

The viviparous aphid Aphis craccivora Koch (Hemiptera: Aphididae) is a serious threat to the crop yield of Lathyrus sativus L. (Fabaceae), commonly known as grass pea. The synthetic insecticides applied to control this insect pest are not safe for the environment. Hence, it is necessary to find volatile organic compounds (VOCs) from two cultivars [BIO L 212 Ratan (BIO) and Nirmal B-1 (NIR)] of L. sativus plants causing behavioral preference of A. craccivora. The VOCs from undamaged (UD), insect-damaged (ID) [plants on which 50 or 100 adults of A. craccivora were fed for 4 h (ID 50 or ID 100)], and mechanically damaged (MD) plants were identified and quantified by gas chromatography-mass spectrometry and gas chromatography-flame ionization detection analyses, respectively. Total VOCs were higher in ID plants compared to UD plants of each cultivar. However, total VOCs were higher in NIR cultivar compared to BIO cultivar for both UD and ID plants. Benzyl alcohol was predominant in volatile extracts of all treatments. In Y-tube olfactometer bioassays, females showed preference towards volatile extracts of UD, ID, and MD plants of each cultivar compared to the control solvent (CH2 Cl2 ). Insects preferred certain synthetic blends comparable to volatile extracts of UD, ID, and MD plants of each L. sativus cultivar against the control solvent. Females preferred a synthetic blend of benzyl alcohol, 1,3-diethylbenzene, thymol, and 1-hexadecene at ratios of 142.49: 62.03:1.18:1 dissolved in 25 μL of CH2 Cl2 in olfactometer bioassays, which could be used in developing lures to control this insect pest.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Acetophenone, ReagentPlus®, 99%
Sigma-Aldrich
Myrcene, sum of isomers, ≥90%, natural, FG
Supelco
1,3-Diethylbenzene, ≥98.0% (GC)
Sigma-Aldrich
Linalool oxide, mixture of isomers, ≥97.0% (GC)
Sigma-Aldrich
p-Cymene, 99%
Sigma-Aldrich
1-Hexadecene, ≥98.5%
Sigma-Aldrich
1-Hexanol, anhydrous, ≥99%
Sigma-Aldrich
4-Hydroxy-4-methyl-2-pentanone, 99%
Sigma-Aldrich
Thymol, ≥98.5%
Sigma-Aldrich
2-Heptanone, 99%
Sigma-Aldrich
Benzyl alcohol, anhydrous, 99.8%
Sigma-Aldrich
Decanal, natural, ≥97%, FG
Sigma-Aldrich
trans-2-Penten-1-ol, 95%