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  • Serum amyloid P component is an essential element of resistance against Aspergillus fumigatus.

Serum amyloid P component is an essential element of resistance against Aspergillus fumigatus.

Nature communications (2021-06-20)
Andrea Doni, Raffaella Parente, Ilaria Laface, Elena Magrini, Cristina Cunha, Federico Simone Colombo, João F Lacerda, António Campos, Sarah N Mapelli, Francesca Petroni, Rémi Porte, Tilo Schorn, Antonio Inforzato, Toine Mercier, Katrien Lagrou, Johan Maertens, John D Lambris, Barbara Bottazzi, Cecilia Garlanda, Marina Botto, Agostinho Carvalho, Alberto Mantovani
ABSTRACT

Serum amyloid P component (SAP, also known as Pentraxin 2; APCS gene) is a component of the humoral arm of innate immunity involved in resistance to bacterial infection and regulation of tissue remodeling. Here we investigate the role of SAP in antifungal resistance. Apcs-/- mice show enhanced susceptibility to A. fumigatus infection. Murine and human SAP bound conidia, activate the complement cascade and enhance phagocytosis by neutrophils. Apcs-/- mice are defective in vivo in terms of recruitment of neutrophils and phagocytosis in the lungs. Opsonic activity of SAP is dependent on the classical pathway of complement activation. In immunosuppressed mice, SAP administration protects hosts against A. fumigatus infection and death. In the context of a study of hematopoietic stem-cell transplantation, genetic variation in the human APCS gene is associated with susceptibility to invasive pulmonary aspergillosis. Thus, SAP is a fluid phase pattern recognition molecule essential for resistance against A. fumigatus.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Serum Amyloid P Component Rabbit pAb, liquid, Calbiochem®
Sigma-Aldrich
Anti-FcγRII (human) Antibody, clone AT10, clone AT10, from mouse
Sigma-Aldrich
Anti-Vinculin antibody, Mouse monoclonal, clone hVIN-1, purified from hybridoma cell culture