MilliporeSigma
  • Long-term epilepsy-associated tumors: transcriptional signatures reflect clinical course.

Long-term epilepsy-associated tumors: transcriptional signatures reflect clinical course.

Scientific reports (2020-01-11)
Daniel Delev, Karam Daka, Sabrina Heynckes, Annette Gaebelein, Pamela Franco, Dietmar Pfeifer, Marco Prinz, Oliver Schnell, Horst Urbach, Irina Mader, Jürgen Beck, Alexander Grote, Albert J Becker, Dieter Henrik Heiland
ABSTRACT

Long-term epilepsy-associated tumors (LEATs) represent mostly benign brain tumors associated with drug-resistant epilepsy. The aim of the study was to investigate the specific transcriptional signatures of those tumors and characterize their underlying oncogenic drivers. A cluster analysis of 65 transcriptome profiles from three independent datasets resulted in four distinct transcriptional subgroups. The first subgroup revealed transcriptional activation of STAT3 and TGF-signaling pathways and contained predominantly dysembryoplastic neuroepithelial tumors (DNTs). The second subgroup was characterized by alterations in the MAPK-pathway and up-stream cascades including FGFR and EGFR-mediated signaling. This tumor cluster exclusively contained neoplasms with somatic BRAFV600E mutations and abundance of gangliogliomas (GGs) with a significantly higher recurrence rate (42%). This finding was validated by examining recurrent tumors from the local database exhibiting BRAFV600E in 90% of the cases. The third cluster included younger patients with neuropathologically diagnosed GGs and abundance of the NOTCH- and mTOR-signaling pathways. The transcript signature of the fourth cluster (including both DNTs and GGs) was related to impaired neural function. Our analysis suggests distinct oncological pathomechanisms in long-term epilepsy-associated tumors. Transcriptional activation of MAPK-pathway and BRAFV600E mutation are associated with an increased risk for tumor recurrence and malignant progression, therefore the treatment of these tumors should integrate both epileptological and oncological aspects.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-BRAF (V600E) antibody, Rabbit monoclonal, recombinant, expressed in HEK 293 cells, clone RM8, purified immunoglobulin