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  • Identifying Protein Interactions with Histone Peptides Using Bio-layer Interferometry.

Identifying Protein Interactions with Histone Peptides Using Bio-layer Interferometry.

Bio-protocol (2018-09-20)
Bingbing Ren, Ahmed Mahmoud Mohammed Sayed, Hwei Ling Tan, Yu Keung Mok, Ee Sin Chen
ABSTRACT

Histone post-translational modifications (PTMs) regulate numerous cellular processes, including gene transcription, cell division, and DNA damage repair. Most histone PTMs affect the recruitment or exclusion of reader proteins from chromatin. Here, we present a protocol to measure affinity and interaction kinetics between histone peptides and the recombinant protein using Bio-layer interferometry.

MATERIALS
Product Number
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Product Description

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