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  • Generation of iPSC line from a Parkinson patient with PARK7 mutation and CRISPR-edited Gibco human episomal iPSC line to mimic PARK7 mutation.

Generation of iPSC line from a Parkinson patient with PARK7 mutation and CRISPR-edited Gibco human episomal iPSC line to mimic PARK7 mutation.

Stem cell research (2021-08-23)
Melissa Conti Mazza, Alexandra Beilina, Dorien A Roosen, David Hauser, Mark R Cookson
ABSTRACT

Mutations in the oncogene PARK7, which codes for DJ-1, have been associated with early-onset autosomal recessive Parkinson's disease (PD); however, the exact role of DJ-1 in PD remains elusive. Fibroblasts from a PD patient with a uniparental disomy, 1 bp deletion in PARK7 were reprogrammed into the induced pluripotent stem cell (iPSC) line: NIHTVBi015-A. For control purposes, CRISPR-Cas9 editing was used to mimic the mutation in the Gibco Human Episomal iPSC line: TMOi001-A is the control line (A18945) and TMOi001-A-3 is the control-edited line (2B10). All 3 lines exhibit normal karyotyping and expression of pluripotent markers: OCT4, SOX2, and NANOG. These lines provide a translational environment to study DJ-1-related function in PD.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2, Alexa Fluor 488 conjugate, clone 7F9.2, from mouse, ALEXA FLUOR 488
Sigma-Aldrich
Anti-NANOG Antibody, clone 7F7.1, Alexa Fluor 488 conjugate, clone 7F7.1, from mouse, ALEXA FLUOR 488
Sigma-Aldrich
Fluorescent Human ES/iPS Cell Characterization Kit, This Fluorescent Human ES/iPS Cell Characterization Kit contains a range of sensitive tools for the phenotypic assessment of the pluripotent status of human Embryonic stem & induced pluripotent Stem cells.