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  • Polarized Two-Photon Absorption and Heterogeneous Fluorescence Dynamics in NAD(P)H.

Polarized Two-Photon Absorption and Heterogeneous Fluorescence Dynamics in NAD(P)H.

The journal of physical chemistry. B (2019-04-26)
Thomas S Blacker, Nick Nicolaou, Michael R Duchen, Angus J Bain
ABSTRACT

Two-photon absorption (2PA) finds widespread application in biological systems, which frequently exhibit heterogeneous fluorescence decay dynamics corresponding to multiple species or environments. By combining polarized 2PA with time-resolved fluorescence intensity and anisotropy decay measurements, we show how the two-photon transition tensors for the components of a heterogeneous population can be separately determined, allowing structural differences between the two fluorescent states of the redox cofactor NAD(P)H to be identified. The results support the view that the two states correspond to alternate configurations of the nicotinamide ring, rather than folded and extended conformations of the entire molecule.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
β-Nicotinamide adenine dinucleotide, reduced disodium salt hydrate, ≥97% (HPLC)
Sigma-Aldrich
1,4-Bis(2-methylstyryl)benzene, BioReagent, suitable for scintillation, ≥98% (UV)