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  • Serum amyloid A protein enhances the activity of secretory non-pancreatic phospholipase A2.

Serum amyloid A protein enhances the activity of secretory non-pancreatic phospholipase A2.

The Biochemical journal (1995-07-15)
W Pruzanski, F C de Beer, M C de Beer, E Stefanski, P Vadas
ABSTRACT

The acute-phase proteins serum amyloid A protein (SAA) and secretory phospholipase A2 (sPLA2) are simultaneously expressed during inflammatory conditions. SAA associates with high-density lipoprotein (HDL) altering its physicochemical composition. We found that purified acute-phase SAA, but not the constitutive form, markedly enhances the lipolytic activity of sPLA2 in a dose-related manner with phosphatidylcholine/lysophosphatidylcholine or phosphatidylethanolamine/lysophosphatidylethanolamine liposomal substrates. Normal HDL was found to reduce activity of sPLA2 in a dose-dependent manner, but when acute-phase HDL containing 27% SAA was tested, it enhanced sPLA2 activity. Immunopurified monospecific antibodies against SAA completely abolished the enhancing activity of SAA and acute-phase HDL. Given the central role of HDL in lipoprotein metabolism, the interaction between HDL, SAA and sPLA2 may account for changes detected in lipoprotein metabolism during the acute phase.

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Monoclonal Anti-β2-Microglobulin antibody produced in mouse, clone BM-63, ascites fluid