Skip to Content
MilliporeSigma

Sex-specific multi-level 3D genome dynamics in the mouse brain.

Nature communications (2022-06-16)
Devin Rocks, Mamta Shukla, Laila Ouldibbat, Silvia C Finnemann, Achyuth Kalluchi, M Jordan Rowley, Marija Kundakovic
ABSTRACT

The female mammalian brain exhibits sex hormone-driven plasticity during the reproductive period. Recent evidence implicates chromatin dynamics in gene regulation underlying this plasticity. However, whether ovarian hormones impact higher-order chromatin organization in post-mitotic neurons in vivo is unknown. Here, we mapped the 3D genome of ventral hippocampal neurons across the oestrous cycle and by sex in mice. In females, we find cycle-driven dynamism in 3D chromatin organization, including in oestrogen response elements-enriched X chromosome compartments, autosomal CTCF loops, and enhancer-promoter interactions. With rising oestrogen levels, the female 3D genome becomes more similar to the male 3D genome. Cyclical enhancer-promoter interactions are partially associated with gene expression and enriched for brain disorder-relevant genes and pathways. Our study reveals unique 3D genome dynamics in the female brain relevant to female-specific gene regulation, neuroplasticity, and disease risk.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Milli-Mark® Mouse IgG1-k, clone MOPC-21, Alexa Fluor 488 conjugate, Mouse IgG1-k Monoclonal Antibody control validated for use in Flow Cytometry.
Sigma-Aldrich
Anti-NeuN Antibody, clone A60, Alexa Fluor488 conjugated, clone A60, Chemicon®, from mouse
Sigma-Aldrich
Anti-Estrogen Receptor α Antibody, from rabbit, purified by affinity chromatography