MilliporeSigma
  • The interplay between SARS-CoV-2 infected airway epithelium and immune cells modulates regulatory/inflammatory signals.

The interplay between SARS-CoV-2 infected airway epithelium and immune cells modulates regulatory/inflammatory signals.

iScience (2022-02-08)
Veronica Bordoni, Giulia Matusali, Davide Mariotti, Manuela Antonioli, Eleonora Cimini, Alessandra Sacchi, Eleonora Tartaglia, Rita Casetti, Germana Grassi, Stefania Notari, Concetta Castilletti, Gian Maria Fimia, Maria Rosaria Capobianchi, Giuseppe Ippolito, Chiara Agrati
ABSTRACT

To assess the cross-talk between immune cells and respiratory tract during SARS-CoV-2 infection, we analyzed the relationships between the inflammatory response induced by SARS-CoV-2 replication and immune cells phenotype in a reconstituted organotypic human airway epithelium (HAE). The results indicated that immune cells failed to inhibit SARS-CoV-2 replication in the HAE model. In contrast, immune cells strongly affected the inflammatory profile induced by SARS-CoV-2 infection, dampening the production of several immunoregulatory/inflammatory signals (e.g., IL-35, IL-27, and IL-34). Moreover, these mediators were found inversely correlated with innate immune cell frequency (NK and γδ T cells) and directly with CD8 T cells. The enriched signals associated with NK and CD8 T cells highlighted the modulation of pathways induced by SARS-CoV-2 infected HAE. These findings are useful to depict the cell-cell communication mechanisms necessary to develop novel therapeutic strategies aimed to promote an effective immune response.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Rabbit IgG (H+L), F(ab′)2 fragment, CF647 antibody produced in goat, ~2 mg/mL, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Anti-Mouse IgG1 (γ1), CF488A antibody produced in goat, ~2 mg/mL, affinity isolated antibody, buffered aqueous solution