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  • Manipulation of self-renewal in human embryonic stem cells through a novel pharmacological GSK-3 inhibitor.

Manipulation of self-renewal in human embryonic stem cells through a novel pharmacological GSK-3 inhibitor.

Methods in molecular biology (Clifton, N.J.) (2006-08-03)
Noboru Sato, Ali H Brivanlou
ABSTRACT

This chapter introduces a new method of maintaining human embryonic stem cells (hESCs) in the undifferentiated state through treatment with a GSK-3 inhibitor, BIO, under a feeder-free condition. Additionally, methods are introduced that determine multidifferentiation potential of hESCs by differentiating into a specific type or random heterogenous cell populations in vitro. These approaches will become a fundamental platform to identify molecular networks regulating cell fate determination in hESCs and mouse embryonic stem cells.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
GSK-3 Inhibitor X, The GSK-3 Inhibitor X, also referenced under CAS 740841-15-0, controls the biological activity of GSK-3. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.
Sigma-Aldrich
Bovine Serum Albumin, heat shock fraction, protease free, suitable for hybridization, pH 7, ≥98%
Sigma-Aldrich
G 418 disulfate salt solution, 50 mg/mL in H2O, 0.1 μm filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
Anti-Keratin Epithelial Antibody, clone AE3, clone AE3, Chemicon®, from mouse