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Common Markers and Small Molecule Inhibitors in Golgi Studies.

Methods in molecular biology (Clifton, N.J.) (2022-12-14)
Sarah Bui, Drew Stark, Jie Li, Jianchao Zhang, Yanzhuang Wang
ABSTRACT

In this chapter, we provide a detailed guide for the application of commonly used small molecules to study Golgi structure and function in vitro. Furthermore, we have curated a concise, validated list of endomembrane markers typically used in downstream assays to examine the consequent effect on the Golgi via microscopy and western blot after drug treatment. This chapter will be useful for researchers beginning their foray into the field of intracellular trafficking and Golgi biology.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Chloroquine diphosphate salt, powder or crystals, 98.5-101.0% (EP)
Roche
Antipain dihydrochloride, crystalline, suitable for cell culture, pkg of 10 mg
Sigma-Aldrich
LY-411575, ≥97% (HPLC)
Sigma-Aldrich
Latrunculin B from Latruncula magnifica, ≥80% (HPLC), solid
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KN-93, ≥98% (HPLC)
Sigma-Aldrich
Bisindolylmaleimide I, A highly selective, cell-permeable, and reversible protein kinase C (PKC) inhibitor (IC₅₀ = 10 nM) that is structurally similar to staurosporine.
Sigma-Aldrich
Tunicamycin, Streptomyces lysosuperficus
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Staurosporine, Streptomyces sp., Staurosporine, CAS 62996-74-, is a cell-permeable, potent, reversible, ATP-competitive inhibitor of protein kinases (IC₅₀ = 7, 20, 1.3, 0.7, & 8.5 nM for PKA, CAMK, MLCK, PKC, & PKG, respectively).
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γ-Secretase Inhibitor X, InSolution, ≥90%, 1 mM
Sigma-Aldrich
Lactacystin, ≥90% (HPLC)
Sigma-Aldrich
Anti-α1,2-Mannosidase IA antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Tunicamycin from Streptomyces sp.
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MG-132, ≥95% by HPLC, Potent, reversible, and cell-permeable proteasome inhibitor (Ki = 4 nM).
Millipore
BAPTA/AM, Membrane-permeable form of BAPTA.
Sigma-Aldrich
Fucosyltransferase Inhibitor, 2F-Peracetyl-Fucose, 2F-Peracetyl-Fucose is a cell-permeable fluorinated fucose derivative that acts as an inhibitor of fucosyltransferases following its uptake and metabolic transformation into a GDP-fucose mimetic.
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Pyocyanin, from Pseudomonas aeruginosa, ≥98% (HPLC)
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ML-7, powder
Sigma-Aldrich
FIBRONECTIN FROM HUMAN PLASMA, liquid, 0.1% (Solution), BioReagent, suitable for cell culture
Sigma-Aldrich
N-Acetyl-L-cysteine, Sigma Grade, ≥99% (TLC), powder
Sigma-Aldrich
Rapamycin, ≥95% (HPLC), powder
Sigma-Aldrich
TAPI-1, InSolution, ≥97%, inhibits MMPs and TACE
Sigma-Aldrich
Epoxomicin, ≥95% (HPLC), solid
Sigma-Aldrich
Etoposide, synthetic, 95.0-105.0%, powder
Sigma-Aldrich
β-Secretase Inhibitor II, The β-Secretase Inhibitor II, also referenced under CAS 263563-09-3, controls the biological activity of β-Secretase. This small molecule/inhibitor is primarily used for Neuroscience applications.
Sigma-Aldrich
4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, ≥97.0% (HPLC)
Roche
Chymostatin