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  • Enhanced antitumor efficacy of cisplatin in combination with HemoHIM in tumor-bearing mice.

Enhanced antitumor efficacy of cisplatin in combination with HemoHIM in tumor-bearing mice.

BMC cancer (2009-03-19)
Hae-Ran Park, Eun-Jin Ju, Sung-Kee Jo, Uhee Jung, Sung-Ho Kim, Sung-Tae Yee
ABSTRACT

Although cisplatin is one of the most effective chemotherapeutic agents, cisplatin alone does not achieve a satisfactory therapeutic outcome. Also cisplatin accumulation shows toxicity to normal tissues. In this study, we examined the possibility of HemoHIM both to enhance anticancer effect with cisplatin and to reduce the side effects of cisplatin in melanoma-bearing mice. HemoHIM was prepared by adding the ethanol-insoluble fraction to the total water extract of a mixture of 3 edible herbs, Angelica Radix, Cnidium Rhizoma and Paeonia Radix. Anticancer effects of HemoHIM with cisplatin were evaluated in melanoma-bearing mice. We used a Cr51-release assay to measure the activity of NK/Tc cell and ELISA to evaluate the production of cytokines. In melanoma-bearing mice, cisplatin (4 mg/kg B.W.) reduced the size and weight of the solid tumors, and HemoHIM supplementation with cisplatin enhanced the decrease of both the tumor size (p < 0.1) and weight (p < 0.1). HemoHIM itself did not inhibit melanoma cell growth in vitro, and did not disturb the effects of cisplatin in vitro. However HemoHIM administration enhanced both NK cell and Tc cell activity in mice. Interestingly, HemoHIM increased the proportion of NK cells in the spleen. In melanoma-bearing mice treated with cisplatin, HemoHIM administration also increased the activity of NK cells and Tc cells and the IL-2 and IFN-gamma secretion from splenocytes, which seemed to contribute to the enhanced efficacy of cisplatin by HemoHIM. Also, HemoHIM reduced nephrotoxicity as seen by tubular cell of kidney destruction. HemoHIM may be a beneficial supplement during cisplatin chemotherapy for enhancing the anti-tumor efficacy and reducing the toxicity of cisplatin.

MATERIALS
Product Number
Brand
Product Description

SAFC
Hanks′ Balanced Salt solution, HBSS Modified, with phenol red, without calcium, without magnesium, liquid
SAFC
Hanks′ Balanced Salt solution, HBSS Modified, with calcium, with magnesium, without phenol red, liquid, suitable for cell culture
Sigma-Aldrich
Hanks′ Balanced Salt solution, With sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Hanks′ Balanced Salt solution, 10 ×, Modified, without calcium, magnesium or sodium bicarbonate
Sigma-Aldrich
Hanks′ Balanced Salt solution, Modified, with sodium bicarbonate, without calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Hanks′ Balanced Salt solution, Modified, with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Hanks′ Balanced Salt solution, Modified, with sodium bicarbonate, without phenol red, liquid, sterile-filtered, suitable for cell culture