MilliporeSigma
  • Efficient high-throughput sequencing of a laser microdissected chromosome arm.

Efficient high-throughput sequencing of a laser microdissected chromosome arm.

BMC genomics (2013-05-30)
Eva Seifertova, Lyle B Zimmerman, Michael J Gilchrist, Jaroslav Macha, Svatava Kubickova, Halina Cernohorska, Vojtech Zarsky, Nick D L Owens, Abdul K Sesay, Tereza Tlapakova, Vladimir Krylov
ABSTRACT

Genomic sequence assemblies are key tools for a broad range of gene function and evolutionary studies. The diploid amphibian Xenopus tropicalis plays a pivotal role in these fields due to its combination of experimental flexibility, diploid genome, and early-branching tetrapod taxonomic position, having diverged from the amniote lineage ~360 million years ago. A genome assembly and a genetic linkage map have recently been made available. Unfortunately, large gaps in the linkage map attenuate long-range integrity of the genome assembly. We laser dissected the short arm of X. tropicalis chromosome 7 for next generation sequencing and computational mapping to the reference genome. This arm is of particular interest as it encodes the sex determination locus, but its genetic map contains large gaps which undermine available genome assemblies. Whole genome amplification of 15 laser-microdissected 7p arms followed by next generation sequencing yielded ~35 million reads, over four million of which uniquely mapped to the X. tropicalis genome. Our analysis placed more than 200 previously unmapped scaffolds on the analyzed chromosome arm, providing valuable low-resolution physical map information for de novo genome assembly. We present a new approach for improving and validating genetic maps and sequence assemblies. Whole genome amplification of 15 microdissected chromosome arms provided sufficient high-quality material for localizing previously unmapped scaffolds and genes as well as recognizing mislocalized scaffolds.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
GenomePlex® WGA Reamplification Kit, Reamplification of WGA product with minimal bias
Sigma-Aldrich
GenomePlex® Single Cell Whole Genome Amplification Kit, Amplify genome of a single cell