MilliporeSigma

Enumeration of Bacillus cereus in foods.

Applied microbiology (1967-05-01)
D A Mossel, M J Koopman, E Jongerius
ABSTRACT

For the enumeration of vegetative cells and spores of Bacillus cereus in foods, a mannitol-egg yolk-phenol red-agar has been developed which exploits the failure of B. cereus to dissimilate mannitol, and the ability of most strains to produce phospholipase C. When a high degree of selectivity was required, polymyxin B sulfate in a concentration of 10 ppm appeared to be the most effective selective additive. Useful characteristics for the identification of presumptive isolates of B. cereus were found to be: morphology, dissimilation of glucose mostly to acetyl methyl carbinol under anaerobic conditions, hydrolysis of starch and gelatin, reduction of nitrate, and growth on 0.25% chloral hydrate agar.

MATERIALS
Product Number
Brand
Product Description

Millipore
Cereus Selective Agar, NutriSelect® Basic, suitable for microbiology